Beneficial effects of omega-3 fatty acids in the proteome of high-density lipoprotein proteome
<p>Abstract</p> <p>Background</p> <p>Omega-3 poly-unsaturated fatty acids (ω-3 PUFAs) have demonstrated to be beneficial in the prevention of cardiovascular disease, however, the mechanisms by which they perform their cardiovascular protection have not been clarified. I...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-09-01
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| Series: | Lipids in Health and Disease |
| Subjects: | |
| Online Access: | http://www.lipidworld.com/content/11/1/116 |
| _version_ | 1818757577040723968 |
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| author | Burillo Elena Mateo-Gallego Rocío Cenarro Ana Fiddyment Sarah Bea Ana M Jorge Inmaculada Vázquez Jesús Civeira Fernando |
| author_facet | Burillo Elena Mateo-Gallego Rocío Cenarro Ana Fiddyment Sarah Bea Ana M Jorge Inmaculada Vázquez Jesús Civeira Fernando |
| author_sort | Burillo Elena |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Omega-3 poly-unsaturated fatty acids (ω-3 PUFAs) have demonstrated to be beneficial in the prevention of cardiovascular disease, however, the mechanisms by which they perform their cardiovascular protection have not been clarified. Intriguingly, some of these protective effects have also been linked to HDL. The hypothesis of this study was that ω-3 PUFAs could modify the protein cargo of HDL particle in a triglyceride non-dependent mode. The objective of the study was to compare the proteome of HDL before and after ω-3 PUFAs supplemented diet.</p> <p>Methods</p> <p>A comparative proteomic analysis in 6 smoker subjects HDL before and after a 5 weeks ω-3 PUFAs enriched diet has been performed.</p> <p>Results</p> <p>Among the altered proteins, clusterin, paraoxonase, and apoAI were found to increase, while fibronectin, α-1-antitrypsin, complement C1r subcomponent and complement factor H decreased after diet supplementation with ω-3 PUFAs. Immunodetection assays confirmed these results. The up-regulated proteins are related to anti-oxidant, anti-inflammatory and anti-atherosclerotic properties of HDL, while the down-regulated proteins are related to regulation of complement activation and acute phase response.</p> <p>Conclusions</p> <p>Despite the low number of subjects included in the study, our findings demonstrate that ω-3 PUFAs supplementation modifies lipoprotein containing apoAI (LpAI) proteome and suggest that these protein changes improve the functionality of the particle.</p> |
| first_indexed | 2024-12-18T06:13:08Z |
| format | Article |
| id | doaj.art-8510e14602db495a808dd85a6f7b4d39 |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-12-18T06:13:08Z |
| publishDate | 2012-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-8510e14602db495a808dd85a6f7b4d392022-12-21T21:18:20ZengBMCLipids in Health and Disease1476-511X2012-09-0111111610.1186/1476-511X-11-116Beneficial effects of omega-3 fatty acids in the proteome of high-density lipoprotein proteomeBurillo ElenaMateo-Gallego RocíoCenarro AnaFiddyment SarahBea Ana MJorge InmaculadaVázquez JesúsCiveira Fernando<p>Abstract</p> <p>Background</p> <p>Omega-3 poly-unsaturated fatty acids (ω-3 PUFAs) have demonstrated to be beneficial in the prevention of cardiovascular disease, however, the mechanisms by which they perform their cardiovascular protection have not been clarified. Intriguingly, some of these protective effects have also been linked to HDL. The hypothesis of this study was that ω-3 PUFAs could modify the protein cargo of HDL particle in a triglyceride non-dependent mode. The objective of the study was to compare the proteome of HDL before and after ω-3 PUFAs supplemented diet.</p> <p>Methods</p> <p>A comparative proteomic analysis in 6 smoker subjects HDL before and after a 5 weeks ω-3 PUFAs enriched diet has been performed.</p> <p>Results</p> <p>Among the altered proteins, clusterin, paraoxonase, and apoAI were found to increase, while fibronectin, α-1-antitrypsin, complement C1r subcomponent and complement factor H decreased after diet supplementation with ω-3 PUFAs. Immunodetection assays confirmed these results. The up-regulated proteins are related to anti-oxidant, anti-inflammatory and anti-atherosclerotic properties of HDL, while the down-regulated proteins are related to regulation of complement activation and acute phase response.</p> <p>Conclusions</p> <p>Despite the low number of subjects included in the study, our findings demonstrate that ω-3 PUFAs supplementation modifies lipoprotein containing apoAI (LpAI) proteome and suggest that these protein changes improve the functionality of the particle.</p>http://www.lipidworld.com/content/11/1/116Omega-3 poly-unsaturated fatty acidsHDL proteomeCardiovascular risk |
| spellingShingle | Burillo Elena Mateo-Gallego Rocío Cenarro Ana Fiddyment Sarah Bea Ana M Jorge Inmaculada Vázquez Jesús Civeira Fernando Beneficial effects of omega-3 fatty acids in the proteome of high-density lipoprotein proteome Lipids in Health and Disease Omega-3 poly-unsaturated fatty acids HDL proteome Cardiovascular risk |
| title | Beneficial effects of omega-3 fatty acids in the proteome of high-density lipoprotein proteome |
| title_full | Beneficial effects of omega-3 fatty acids in the proteome of high-density lipoprotein proteome |
| title_fullStr | Beneficial effects of omega-3 fatty acids in the proteome of high-density lipoprotein proteome |
| title_full_unstemmed | Beneficial effects of omega-3 fatty acids in the proteome of high-density lipoprotein proteome |
| title_short | Beneficial effects of omega-3 fatty acids in the proteome of high-density lipoprotein proteome |
| title_sort | beneficial effects of omega 3 fatty acids in the proteome of high density lipoprotein proteome |
| topic | Omega-3 poly-unsaturated fatty acids HDL proteome Cardiovascular risk |
| url | http://www.lipidworld.com/content/11/1/116 |
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