A graphitic nano-onion/molybdenum disulfide nanosheet composite as a platform for HPV-associated cancer-detecting DNA biosensors
Abstract An electrochemical DNA sensor that can detect human papillomavirus (HPV)-16 and HPV-18 for the early diagnosis of cervical cancer was developed by using a graphitic nano-onion/molybdenum disulfide (MoS2) nanosheet composite. The electrode surface for probing DNA chemisorption was prepared v...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-06-01
|
| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-023-01948-6 |
| _version_ | 1797806518585786368 |
|---|---|
| author | Youngjun Kim Eunah Kang |
| author_facet | Youngjun Kim Eunah Kang |
| author_sort | Youngjun Kim |
| collection | DOAJ |
| description | Abstract An electrochemical DNA sensor that can detect human papillomavirus (HPV)-16 and HPV-18 for the early diagnosis of cervical cancer was developed by using a graphitic nano-onion/molybdenum disulfide (MoS2) nanosheet composite. The electrode surface for probing DNA chemisorption was prepared via chemical conjugation between acyl bonds on the surfaces of functionalized nanoonions and the amine groups on functionalized MoS2 nanosheets. The cyclic voltammetry profile of an 1:1 nanoonion/MoS2 nanosheet composite electrode had an improved rectangular shape compared to that of an MoS2 nanosheet elecrode, thereby indicating the amorphous nature of the nano-onions with sp2 distancing curved carbon layers that provide enhanced electronic conductivity, compared to MoS2 nanosheet only. The nanoonion/MoS2 sensor for the DNA detection of HPV-16 and HPV-18, respectively, was measured at high sensitivity through differential pulse voltammetry (DPV) in the presence of methylene blue (MB) as a redox indicator. The DPV current peak was lowered after probe DNA chemisorption and target DNA hybridization because the hybridized DNA induced less effective MB electrostatic intercalation due to it being double-stranded, resulting in a lower oxidation peak. The nanoonion/MoS2 nanosheet composite electrodes attained higher current peaks than the MoS2 nanosheet electrode, thereby indicating a greater change in the differential peak probably because the nanoonions enhanced conductive electron transfer. Notably, both of the target DNAs produced from HPV-18 and HPV-16 Siha and Hela cancer cell lines were effectively detected with high specificity. The conductivity of MoS2 improved by complexation with nano-onions provides a suitable platform for electrochemical biosensors for the early diagnosis of many ailments in humans. Graphical Abstract |
| first_indexed | 2024-03-13T06:08:33Z |
| format | Article |
| id | doaj.art-8517446a4f074e4f8946d4918efb71bd |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-03-13T06:08:33Z |
| publishDate | 2023-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-8517446a4f074e4f8946d4918efb71bd2023-06-11T11:24:25ZengBMCJournal of Nanobiotechnology1477-31552023-06-0121111610.1186/s12951-023-01948-6A graphitic nano-onion/molybdenum disulfide nanosheet composite as a platform for HPV-associated cancer-detecting DNA biosensorsYoungjun Kim0Eunah Kang1School of Chemical Engineering and Material Science, Chung-Ang UniversitySchool of Chemical Engineering and Material Science, Chung-Ang UniversityAbstract An electrochemical DNA sensor that can detect human papillomavirus (HPV)-16 and HPV-18 for the early diagnosis of cervical cancer was developed by using a graphitic nano-onion/molybdenum disulfide (MoS2) nanosheet composite. The electrode surface for probing DNA chemisorption was prepared via chemical conjugation between acyl bonds on the surfaces of functionalized nanoonions and the amine groups on functionalized MoS2 nanosheets. The cyclic voltammetry profile of an 1:1 nanoonion/MoS2 nanosheet composite electrode had an improved rectangular shape compared to that of an MoS2 nanosheet elecrode, thereby indicating the amorphous nature of the nano-onions with sp2 distancing curved carbon layers that provide enhanced electronic conductivity, compared to MoS2 nanosheet only. The nanoonion/MoS2 sensor for the DNA detection of HPV-16 and HPV-18, respectively, was measured at high sensitivity through differential pulse voltammetry (DPV) in the presence of methylene blue (MB) as a redox indicator. The DPV current peak was lowered after probe DNA chemisorption and target DNA hybridization because the hybridized DNA induced less effective MB electrostatic intercalation due to it being double-stranded, resulting in a lower oxidation peak. The nanoonion/MoS2 nanosheet composite electrodes attained higher current peaks than the MoS2 nanosheet electrode, thereby indicating a greater change in the differential peak probably because the nanoonions enhanced conductive electron transfer. Notably, both of the target DNAs produced from HPV-18 and HPV-16 Siha and Hela cancer cell lines were effectively detected with high specificity. The conductivity of MoS2 improved by complexation with nano-onions provides a suitable platform for electrochemical biosensors for the early diagnosis of many ailments in humans. Graphical Abstracthttps://doi.org/10.1186/s12951-023-01948-6Nano-onionHPVDNAElectrochemical biosensorMolybdenum disulfideAmorphous nanocarbon supports |
| spellingShingle | Youngjun Kim Eunah Kang A graphitic nano-onion/molybdenum disulfide nanosheet composite as a platform for HPV-associated cancer-detecting DNA biosensors Journal of Nanobiotechnology Nano-onion HPV DNA Electrochemical biosensor Molybdenum disulfide Amorphous nanocarbon supports |
| title | A graphitic nano-onion/molybdenum disulfide nanosheet composite as a platform for HPV-associated cancer-detecting DNA biosensors |
| title_full | A graphitic nano-onion/molybdenum disulfide nanosheet composite as a platform for HPV-associated cancer-detecting DNA biosensors |
| title_fullStr | A graphitic nano-onion/molybdenum disulfide nanosheet composite as a platform for HPV-associated cancer-detecting DNA biosensors |
| title_full_unstemmed | A graphitic nano-onion/molybdenum disulfide nanosheet composite as a platform for HPV-associated cancer-detecting DNA biosensors |
| title_short | A graphitic nano-onion/molybdenum disulfide nanosheet composite as a platform for HPV-associated cancer-detecting DNA biosensors |
| title_sort | graphitic nano onion molybdenum disulfide nanosheet composite as a platform for hpv associated cancer detecting dna biosensors |
| topic | Nano-onion HPV DNA Electrochemical biosensor Molybdenum disulfide Amorphous nanocarbon supports |
| url | https://doi.org/10.1186/s12951-023-01948-6 |
| work_keys_str_mv | AT youngjunkim agraphiticnanoonionmolybdenumdisulfidenanosheetcompositeasaplatformforhpvassociatedcancerdetectingdnabiosensors AT eunahkang agraphiticnanoonionmolybdenumdisulfidenanosheetcompositeasaplatformforhpvassociatedcancerdetectingdnabiosensors AT youngjunkim graphiticnanoonionmolybdenumdisulfidenanosheetcompositeasaplatformforhpvassociatedcancerdetectingdnabiosensors AT eunahkang graphiticnanoonionmolybdenumdisulfidenanosheetcompositeasaplatformforhpvassociatedcancerdetectingdnabiosensors |