MyD88 Mediates Colitis- and RANKL-Induced Microfold Cell Differentiation

Intestinal microfold (M) cells are critical for sampling antigens in the gut and initiating the intestinal mucosal immune response. In this study, we found that the oral administration of dextran sulfate sodium (DSS) and <i>Salmonella</i> infection induced colitis. In the process, the ex...

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Main Authors: Yang Li, Shanshan Yang, Xin Huang, Ning Yang, Caiying Wang, Jing Zhao, Zhizhong Jing, Luc Willems, Guangliang Liu
Format: Article
Language:English
Published: MDPI AG 2021-12-01
Series:Veterinary Sciences
Subjects:
Online Access:https://www.mdpi.com/2306-7381/9/1/6
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author Yang Li
Shanshan Yang
Xin Huang
Ning Yang
Caiying Wang
Jing Zhao
Zhizhong Jing
Luc Willems
Guangliang Liu
author_facet Yang Li
Shanshan Yang
Xin Huang
Ning Yang
Caiying Wang
Jing Zhao
Zhizhong Jing
Luc Willems
Guangliang Liu
author_sort Yang Li
collection DOAJ
description Intestinal microfold (M) cells are critical for sampling antigens in the gut and initiating the intestinal mucosal immune response. In this study, we found that the oral administration of dextran sulfate sodium (DSS) and <i>Salmonella</i> infection induced colitis. In the process, the expression levels of M cell differentiation-related genes were synchronized with the kinetics of pro-inflammatory cytokines. Compared to wild-type (WT) mice, <i>MyD88</i><sup>−/−</sup> mice exhibited significantly lower expression levels of M cell differentiation-related genes. However, DSS induced colitis in <i>MyD88</i><sup>−/−</sup> mice but failed to promote the transcription of M cell differentiation related genes. Furthermore, the receptor activator of the Nuclear Factor-κB ligand (RANKL) upregulated the transcription of M cell differentiation related genes in murine intestinal organoids prepared from both WT and <i>MyD88</i><sup>−/−</sup> mice. Meanwhile, fewer changes in M cell differentiation related genes were found in <i>MyD88</i><sup>−/−</sup> mice as compared to WT mice. Hence, we concluded that myeloid differentiation factor 88 (MyD88) is an essential molecule for colitis- and RANKL-related differentiation of M cells.
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spelling doaj.art-851796c5ee2149f186a07eae59ce23462023-11-23T15:40:06ZengMDPI AGVeterinary Sciences2306-73812021-12-0191610.3390/vetsci9010006MyD88 Mediates Colitis- and RANKL-Induced Microfold Cell DifferentiationYang Li0Shanshan Yang1Xin Huang2Ning Yang3Caiying Wang4Jing Zhao5Zhizhong Jing6Luc Willems7Guangliang Liu8State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Lanzhou 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Lanzhou 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Lanzhou 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Lanzhou 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Lanzhou 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Lanzhou 730046, ChinaState Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Lanzhou 730046, ChinaMolecular and Cellular Epigenetics (GIGA), University of Liege, 4000 Liege, BelgiumState Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Science, Lanzhou 730046, ChinaIntestinal microfold (M) cells are critical for sampling antigens in the gut and initiating the intestinal mucosal immune response. In this study, we found that the oral administration of dextran sulfate sodium (DSS) and <i>Salmonella</i> infection induced colitis. In the process, the expression levels of M cell differentiation-related genes were synchronized with the kinetics of pro-inflammatory cytokines. Compared to wild-type (WT) mice, <i>MyD88</i><sup>−/−</sup> mice exhibited significantly lower expression levels of M cell differentiation-related genes. However, DSS induced colitis in <i>MyD88</i><sup>−/−</sup> mice but failed to promote the transcription of M cell differentiation related genes. Furthermore, the receptor activator of the Nuclear Factor-κB ligand (RANKL) upregulated the transcription of M cell differentiation related genes in murine intestinal organoids prepared from both WT and <i>MyD88</i><sup>−/−</sup> mice. Meanwhile, fewer changes in M cell differentiation related genes were found in <i>MyD88</i><sup>−/−</sup> mice as compared to WT mice. Hence, we concluded that myeloid differentiation factor 88 (MyD88) is an essential molecule for colitis- and RANKL-related differentiation of M cells.https://www.mdpi.com/2306-7381/9/1/6colonic M cellsMyD88colitisDSS
spellingShingle Yang Li
Shanshan Yang
Xin Huang
Ning Yang
Caiying Wang
Jing Zhao
Zhizhong Jing
Luc Willems
Guangliang Liu
MyD88 Mediates Colitis- and RANKL-Induced Microfold Cell Differentiation
Veterinary Sciences
colonic M cells
MyD88
colitis
DSS
title MyD88 Mediates Colitis- and RANKL-Induced Microfold Cell Differentiation
title_full MyD88 Mediates Colitis- and RANKL-Induced Microfold Cell Differentiation
title_fullStr MyD88 Mediates Colitis- and RANKL-Induced Microfold Cell Differentiation
title_full_unstemmed MyD88 Mediates Colitis- and RANKL-Induced Microfold Cell Differentiation
title_short MyD88 Mediates Colitis- and RANKL-Induced Microfold Cell Differentiation
title_sort myd88 mediates colitis and rankl induced microfold cell differentiation
topic colonic M cells
MyD88
colitis
DSS
url https://www.mdpi.com/2306-7381/9/1/6
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