Quantitative real-time PCR for the clinical detection of Helicobacter pylori
Accurate diagnosis of Helicobacter pylori infection is very important in both clinical practice and research. We evaluated the sensitivity of real-time PCR (RT-PCR) for the detection and quantification of Helicobacter pylori using DNA from 91 human gastric biopsy samples divided into three groups: 4...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2007-03-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300022 |
| _version_ | 1818585557063696384 |
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| author | Marcelo Lima Ribeiro Christina Cunha Ecclissato Ricardo Gabriel Mattos Sergio Mendonca José Pedrazzoli Jr. |
| author_facet | Marcelo Lima Ribeiro Christina Cunha Ecclissato Ricardo Gabriel Mattos Sergio Mendonca José Pedrazzoli Jr. |
| author_sort | Marcelo Lima Ribeiro |
| collection | DOAJ |
| description | Accurate diagnosis of Helicobacter pylori infection is very important in both clinical practice and research. We evaluated the sensitivity of real-time PCR (RT-PCR) for the detection and quantification of Helicobacter pylori using DNA from 91 human gastric biopsy samples divided into three groups: 46 biopsies from untreated patients who according to the references methods were considered H. pylori-negative (group A); 35 biopsies from patients previously treated against H. pylori and considered to be cured by "gold standard" tests (group B); and 10 biopsies from patients H. pylori-positive by all available methods (group C). The sensitivity of the RT-PCR assay was higher than that of standard methods. Of the 81 patients considered to be uninfected according to the references methods, 16 were H. pylori-positive by PCR, 10 of which were patients who had received H. pylori eradication therapy and 6 were untreated patients. Based on these findings we recommend that RT-PCR should be use in addition to standard methods in clinical studies to monitor the results of H. pylori eradication therapy. |
| first_indexed | 2024-12-16T08:38:57Z |
| format | Article |
| id | doaj.art-851aabf3fdca4938a2d6e8298f06131b |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-16T08:38:57Z |
| publishDate | 2007-03-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-851aabf3fdca4938a2d6e8298f06131b2022-12-21T22:37:43ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852007-03-0130243143410.1590/S1415-47572007000300022Quantitative real-time PCR for the clinical detection of Helicobacter pyloriMarcelo Lima RibeiroChristina Cunha EcclissatoRicardo Gabriel MattosSergio MendoncaJosé Pedrazzoli Jr.Accurate diagnosis of Helicobacter pylori infection is very important in both clinical practice and research. We evaluated the sensitivity of real-time PCR (RT-PCR) for the detection and quantification of Helicobacter pylori using DNA from 91 human gastric biopsy samples divided into three groups: 46 biopsies from untreated patients who according to the references methods were considered H. pylori-negative (group A); 35 biopsies from patients previously treated against H. pylori and considered to be cured by "gold standard" tests (group B); and 10 biopsies from patients H. pylori-positive by all available methods (group C). The sensitivity of the RT-PCR assay was higher than that of standard methods. Of the 81 patients considered to be uninfected according to the references methods, 16 were H. pylori-positive by PCR, 10 of which were patients who had received H. pylori eradication therapy and 6 were untreated patients. Based on these findings we recommend that RT-PCR should be use in addition to standard methods in clinical studies to monitor the results of H. pylori eradication therapy.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300022diagnostic methodsHelicobacter pylorireal-time PCR |
| spellingShingle | Marcelo Lima Ribeiro Christina Cunha Ecclissato Ricardo Gabriel Mattos Sergio Mendonca José Pedrazzoli Jr. Quantitative real-time PCR for the clinical detection of Helicobacter pylori Genetics and Molecular Biology diagnostic methods Helicobacter pylori real-time PCR |
| title | Quantitative real-time PCR for the clinical detection of Helicobacter pylori |
| title_full | Quantitative real-time PCR for the clinical detection of Helicobacter pylori |
| title_fullStr | Quantitative real-time PCR for the clinical detection of Helicobacter pylori |
| title_full_unstemmed | Quantitative real-time PCR for the clinical detection of Helicobacter pylori |
| title_short | Quantitative real-time PCR for the clinical detection of Helicobacter pylori |
| title_sort | quantitative real time pcr for the clinical detection of helicobacter pylori |
| topic | diagnostic methods Helicobacter pylori real-time PCR |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300022 |
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