Nogo receptor is involved in the adhesion of dendritic cells to myelin
<p>Abstract</p> <p>Background</p> <p>Nogo-66 receptor NgR1 and its structural homologue NgR2 are binding proteins for a number of myelin-associated inhibitory factors. After neuronal injury, these inhibitory factors are responsible for preventing axonal outgrowth via th...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2011-09-01
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| Series: | Journal of Neuroinflammation |
| Subjects: | |
| Online Access: | http://www.jneuroinflammation.com/content/8/1/113 |
| _version_ | 1818136475806466048 |
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| author | Martin Roland Schweigreiter Rüdiger Kern Florian Steinbach Karin McDonald Claire L Bandtlow Christine E Reindl Markus |
| author_facet | Martin Roland Schweigreiter Rüdiger Kern Florian Steinbach Karin McDonald Claire L Bandtlow Christine E Reindl Markus |
| author_sort | Martin Roland |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Nogo-66 receptor NgR1 and its structural homologue NgR2 are binding proteins for a number of myelin-associated inhibitory factors. After neuronal injury, these inhibitory factors are responsible for preventing axonal outgrowth via their interactions with NgR1 and NgR2 expressed on neurons. <it>In vitro</it>, cells expressing NgR1/2 are inhibited from adhering to and spreading on a myelin substrate. Neuronal injury also results in the presence of dendritic cells (DCs) in the central nervous system, where they can come into contact with myelin debris. The exact mechanisms of interaction of immune cells with CNS myelin are, however, poorly understood.</p> <p>Methods</p> <p>Human DCs were differentiated from peripheral blood monocytes and mouse DCs were differentiated from wild type and NgR1/NgR2 double knockout bone marrow precursors. NgR1 and NgR2 expression were determined with quantitative real time PCR and immunoblot, and adhesion of cells to myelin was quantified.</p> <p>Results</p> <p>We demonstrate that human immature myeloid DCs express NgR1 and NgR2, which are then down-regulated upon maturation. Human mature DCs also adhere to a much higher extent to a myelin substrate than immature DCs. We observe the same effect when the cells are plated on Nogo-66-His (binding peptide for NgR1), but not on control proteins. Mature DCs taken from <it>Ngr1/2 </it>knockout mice adhere to a much higher extent to myelin compared to wild type mouse DCs. In addition, <it>Ngr1/2 </it>knockout had no effect on <it>in vitro </it>DC differentiation or phenotype.</p> <p>Conclusions</p> <p>These results indicate that a lack of NgR1/2 expression promotes the adhesion of DCs to myelin. This interaction could be important in neuroinflammatory disorders such as multiple sclerosis in which peripheral immune cells come into contact with myelin debris.</p> |
| first_indexed | 2024-12-11T09:41:00Z |
| format | Article |
| id | doaj.art-851ad5e421bf4636a5ba3e0a178fe5a4 |
| institution | Directory Open Access Journal |
| issn | 1742-2094 |
| language | English |
| last_indexed | 2024-12-11T09:41:00Z |
| publishDate | 2011-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Neuroinflammation |
| spelling | doaj.art-851ad5e421bf4636a5ba3e0a178fe5a42022-12-22T01:12:40ZengBMCJournal of Neuroinflammation1742-20942011-09-018111310.1186/1742-2094-8-113Nogo receptor is involved in the adhesion of dendritic cells to myelinMartin RolandSchweigreiter RüdigerKern FlorianSteinbach KarinMcDonald Claire LBandtlow Christine EReindl Markus<p>Abstract</p> <p>Background</p> <p>Nogo-66 receptor NgR1 and its structural homologue NgR2 are binding proteins for a number of myelin-associated inhibitory factors. After neuronal injury, these inhibitory factors are responsible for preventing axonal outgrowth via their interactions with NgR1 and NgR2 expressed on neurons. <it>In vitro</it>, cells expressing NgR1/2 are inhibited from adhering to and spreading on a myelin substrate. Neuronal injury also results in the presence of dendritic cells (DCs) in the central nervous system, where they can come into contact with myelin debris. The exact mechanisms of interaction of immune cells with CNS myelin are, however, poorly understood.</p> <p>Methods</p> <p>Human DCs were differentiated from peripheral blood monocytes and mouse DCs were differentiated from wild type and NgR1/NgR2 double knockout bone marrow precursors. NgR1 and NgR2 expression were determined with quantitative real time PCR and immunoblot, and adhesion of cells to myelin was quantified.</p> <p>Results</p> <p>We demonstrate that human immature myeloid DCs express NgR1 and NgR2, which are then down-regulated upon maturation. Human mature DCs also adhere to a much higher extent to a myelin substrate than immature DCs. We observe the same effect when the cells are plated on Nogo-66-His (binding peptide for NgR1), but not on control proteins. Mature DCs taken from <it>Ngr1/2 </it>knockout mice adhere to a much higher extent to myelin compared to wild type mouse DCs. In addition, <it>Ngr1/2 </it>knockout had no effect on <it>in vitro </it>DC differentiation or phenotype.</p> <p>Conclusions</p> <p>These results indicate that a lack of NgR1/2 expression promotes the adhesion of DCs to myelin. This interaction could be important in neuroinflammatory disorders such as multiple sclerosis in which peripheral immune cells come into contact with myelin debris.</p>http://www.jneuroinflammation.com/content/8/1/113Nogo receptorNgR1NgR2Nogo-66myelin associated glycoproteinMAGmyelindendritic cells |
| spellingShingle | Martin Roland Schweigreiter Rüdiger Kern Florian Steinbach Karin McDonald Claire L Bandtlow Christine E Reindl Markus Nogo receptor is involved in the adhesion of dendritic cells to myelin Journal of Neuroinflammation Nogo receptor NgR1 NgR2 Nogo-66 myelin associated glycoprotein MAG myelin dendritic cells |
| title | Nogo receptor is involved in the adhesion of dendritic cells to myelin |
| title_full | Nogo receptor is involved in the adhesion of dendritic cells to myelin |
| title_fullStr | Nogo receptor is involved in the adhesion of dendritic cells to myelin |
| title_full_unstemmed | Nogo receptor is involved in the adhesion of dendritic cells to myelin |
| title_short | Nogo receptor is involved in the adhesion of dendritic cells to myelin |
| title_sort | nogo receptor is involved in the adhesion of dendritic cells to myelin |
| topic | Nogo receptor NgR1 NgR2 Nogo-66 myelin associated glycoprotein MAG myelin dendritic cells |
| url | http://www.jneuroinflammation.com/content/8/1/113 |
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