Parecoxib reduced ventilation induced lung injury in acute respiratory distress syndrome
Abstract Background Cyclooxygenase-2 (COX-2) contributes to ventilation induced lung injury (VILI) and acute respiratory distress syndrome (ARDS). The objective of present study was to observe the therapeutic effect of parecoxib on VILI in ARDS. Methods In this parallel controlled study performed at...
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| Format: | Article |
| Language: | English |
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BMC
2017-03-01
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| Series: | BMC Pharmacology and Toxicology |
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| Online Access: | http://link.springer.com/article/10.1186/s40360-017-0131-z |
| _version_ | 1819236980840464384 |
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| author | Fan-you Meng Wei Gao Ying-nan Ju |
| author_facet | Fan-you Meng Wei Gao Ying-nan Ju |
| author_sort | Fan-you Meng |
| collection | DOAJ |
| description | Abstract Background Cyclooxygenase-2 (COX-2) contributes to ventilation induced lung injury (VILI) and acute respiratory distress syndrome (ARDS). The objective of present study was to observe the therapeutic effect of parecoxib on VILI in ARDS. Methods In this parallel controlled study performed at Harbin Medical University, China between January 2016 and March 2016, 24 rats were randomly allocated into sham group (S), volume ventilation group/ARDS (VA), parecoxib/volume ventilation group/ARDS (PVA). Rats in the S group only received anesthesia; rats in the VA and PVA group received intravenous injection of endotoxin to induce ARDS, and then received ventilation. Rats in the VA and PVA groups were treated with intravenous injection of saline or parecoxib. The ratio of arterial oxygen pressure to fractional inspired oxygen (PaO2/FiO2), the wet to dry weight ratio of lung tissue, inflammatory factors in serum and bronchoalveolar lavage fluid (BALF), and histopathologic analyses of lung tissue were examined. In addition, survival was calculated at 24 h after VILI. Results Compared to the VA group, in the PVA group, PaO2/FiO2 was significantly increased; lung tissue wet to dry weight ratio; macrophage and neutrophil counts, total protein and neutrophil elastase levels in BALF; tumor necrosis factor-α, interleukin-1β, and prostaglandin E2 levels in BALF and serum; and myeloperoxidase (MPO) activity, malondialdehyde levels, and Bax and COX-2 protein levels in lung tissue were significantly decreased, while Bcl-2 protein levels were significantly increased. Lung histopathogical changes and apoptosis were reduced by parecpxib in the PVA group. Survival was increased in the PVA group. Conclusions Parecoxib improves gas exchange and epithelial permeability, decreases edema, reduces local and systemic inflammation, ameliorates lung injury and apoptosis, and increases survival in a rat model of VILI. |
| first_indexed | 2024-12-23T13:13:04Z |
| format | Article |
| id | doaj.art-85203dac5a9e4fcea418465f9d960e49 |
| institution | Directory Open Access Journal |
| issn | 2050-6511 |
| language | English |
| last_indexed | 2024-12-23T13:13:04Z |
| publishDate | 2017-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pharmacology and Toxicology |
| spelling | doaj.art-85203dac5a9e4fcea418465f9d960e492022-12-21T17:45:41ZengBMCBMC Pharmacology and Toxicology2050-65112017-03-011811910.1186/s40360-017-0131-zParecoxib reduced ventilation induced lung injury in acute respiratory distress syndromeFan-you Meng0Wei Gao1Ying-nan Ju2Department of Anesthesiology, the Second Affiliated Hospital of the Harbin Medical UniversityDepartment of Anesthesiology, the Second Affiliated Hospital of the Harbin Medical UniversityDepartment of Intensive Care Unit, the Third Affiliated Hospital of the Harbin Medical UniversityAbstract Background Cyclooxygenase-2 (COX-2) contributes to ventilation induced lung injury (VILI) and acute respiratory distress syndrome (ARDS). The objective of present study was to observe the therapeutic effect of parecoxib on VILI in ARDS. Methods In this parallel controlled study performed at Harbin Medical University, China between January 2016 and March 2016, 24 rats were randomly allocated into sham group (S), volume ventilation group/ARDS (VA), parecoxib/volume ventilation group/ARDS (PVA). Rats in the S group only received anesthesia; rats in the VA and PVA group received intravenous injection of endotoxin to induce ARDS, and then received ventilation. Rats in the VA and PVA groups were treated with intravenous injection of saline or parecoxib. The ratio of arterial oxygen pressure to fractional inspired oxygen (PaO2/FiO2), the wet to dry weight ratio of lung tissue, inflammatory factors in serum and bronchoalveolar lavage fluid (BALF), and histopathologic analyses of lung tissue were examined. In addition, survival was calculated at 24 h after VILI. Results Compared to the VA group, in the PVA group, PaO2/FiO2 was significantly increased; lung tissue wet to dry weight ratio; macrophage and neutrophil counts, total protein and neutrophil elastase levels in BALF; tumor necrosis factor-α, interleukin-1β, and prostaglandin E2 levels in BALF and serum; and myeloperoxidase (MPO) activity, malondialdehyde levels, and Bax and COX-2 protein levels in lung tissue were significantly decreased, while Bcl-2 protein levels were significantly increased. Lung histopathogical changes and apoptosis were reduced by parecpxib in the PVA group. Survival was increased in the PVA group. Conclusions Parecoxib improves gas exchange and epithelial permeability, decreases edema, reduces local and systemic inflammation, ameliorates lung injury and apoptosis, and increases survival in a rat model of VILI.http://link.springer.com/article/10.1186/s40360-017-0131-zParecoxibVentilation induced lung injuryAcute respiratory distress syndrome |
| spellingShingle | Fan-you Meng Wei Gao Ying-nan Ju Parecoxib reduced ventilation induced lung injury in acute respiratory distress syndrome BMC Pharmacology and Toxicology Parecoxib Ventilation induced lung injury Acute respiratory distress syndrome |
| title | Parecoxib reduced ventilation induced lung injury in acute respiratory distress syndrome |
| title_full | Parecoxib reduced ventilation induced lung injury in acute respiratory distress syndrome |
| title_fullStr | Parecoxib reduced ventilation induced lung injury in acute respiratory distress syndrome |
| title_full_unstemmed | Parecoxib reduced ventilation induced lung injury in acute respiratory distress syndrome |
| title_short | Parecoxib reduced ventilation induced lung injury in acute respiratory distress syndrome |
| title_sort | parecoxib reduced ventilation induced lung injury in acute respiratory distress syndrome |
| topic | Parecoxib Ventilation induced lung injury Acute respiratory distress syndrome |
| url | http://link.springer.com/article/10.1186/s40360-017-0131-z |
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