VAMS-Based Blood Capillary Sampling for Mass Spectrometry-Based Human Metabolomics Studies
Volumetric absorptive microsampling (VAMS) is a recently developed sample collection method that enables single-drop blood collection in a minimally invasive manner. Blood biomolecules can then be extracted and processed for analysis using several analytical platforms. The integration of VAMS with c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/13/2/146 |
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| author | Chiara Volani Christa Malfertheiner Giulia Caprioli Søren Fjelstrup Peter P. Pramstaller Johannes Rainer Giuseppe Paglia |
| author_facet | Chiara Volani Christa Malfertheiner Giulia Caprioli Søren Fjelstrup Peter P. Pramstaller Johannes Rainer Giuseppe Paglia |
| author_sort | Chiara Volani |
| collection | DOAJ |
| description | Volumetric absorptive microsampling (VAMS) is a recently developed sample collection method that enables single-drop blood collection in a minimally invasive manner. Blood biomolecules can then be extracted and processed for analysis using several analytical platforms. The integration of VAMS with conventional mass spectrometry (MS)-based metabolomics approaches is an attractive solution for human studies representing a less-invasive procedure compared to phlebotomy with the additional potential for remote sample collection. However, as we recently demonstrated, VAMS samples require long-term storage at −80 °C. This study investigated the stability of VAMS samples during short-term storage and compared the metabolome obtained from capillary blood collected from the fingertip to those of plasma and venous blood from 22 healthy volunteers. Our results suggest that the blood metabolome collected by VAMS samples is stable at room temperature only for up to 6 h requiring subsequent storage at −80 °C to avoid significant changes in the metabolome. We also demonstrated that capillary blood provides better coverage of the metabolome compared to plasma enabling the analysis of several intracellular metabolites presented in red blood cells. Finally, this work demonstrates that with the appropriate pre-analytical protocol capillary blood can be successfully used for untargeted metabolomics studies. |
| first_indexed | 2024-03-11T08:26:22Z |
| format | Article |
| id | doaj.art-8522fafe357145fa81cf8e8dc371bb4d |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-11T08:26:22Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-8522fafe357145fa81cf8e8dc371bb4d2023-11-16T22:03:32ZengMDPI AGMetabolites2218-19892023-01-0113214610.3390/metabo13020146VAMS-Based Blood Capillary Sampling for Mass Spectrometry-Based Human Metabolomics StudiesChiara Volani0Christa Malfertheiner1Giulia Caprioli2Søren Fjelstrup3Peter P. Pramstaller4Johannes Rainer5Giuseppe Paglia6Institute for Biomedicine, Affiliated to the University of Lübeck, Eurac Research, 39100 Bolzano, ItalyInstitute for Biomedicine, Affiliated to the University of Lübeck, Eurac Research, 39100 Bolzano, ItalyInstitute for Biomedicine, Affiliated to the University of Lübeck, Eurac Research, 39100 Bolzano, ItalyInterdisciplinary Nanoscience Center, Aarhus University, 8000 Aarhus, DenmarkInstitute for Biomedicine, Affiliated to the University of Lübeck, Eurac Research, 39100 Bolzano, ItalyInstitute for Biomedicine, Affiliated to the University of Lübeck, Eurac Research, 39100 Bolzano, ItalySchool of Medicine and Surgery, University of Milano-Bicocca, 20854 Vedano al Lambro, ItalyVolumetric absorptive microsampling (VAMS) is a recently developed sample collection method that enables single-drop blood collection in a minimally invasive manner. Blood biomolecules can then be extracted and processed for analysis using several analytical platforms. The integration of VAMS with conventional mass spectrometry (MS)-based metabolomics approaches is an attractive solution for human studies representing a less-invasive procedure compared to phlebotomy with the additional potential for remote sample collection. However, as we recently demonstrated, VAMS samples require long-term storage at −80 °C. This study investigated the stability of VAMS samples during short-term storage and compared the metabolome obtained from capillary blood collected from the fingertip to those of plasma and venous blood from 22 healthy volunteers. Our results suggest that the blood metabolome collected by VAMS samples is stable at room temperature only for up to 6 h requiring subsequent storage at −80 °C to avoid significant changes in the metabolome. We also demonstrated that capillary blood provides better coverage of the metabolome compared to plasma enabling the analysis of several intracellular metabolites presented in red blood cells. Finally, this work demonstrates that with the appropriate pre-analytical protocol capillary blood can be successfully used for untargeted metabolomics studies.https://www.mdpi.com/2218-1989/13/2/146VAMSuntargeted metabolomicscapillary bloodsample collectionmass spectrometry |
| spellingShingle | Chiara Volani Christa Malfertheiner Giulia Caprioli Søren Fjelstrup Peter P. Pramstaller Johannes Rainer Giuseppe Paglia VAMS-Based Blood Capillary Sampling for Mass Spectrometry-Based Human Metabolomics Studies Metabolites VAMS untargeted metabolomics capillary blood sample collection mass spectrometry |
| title | VAMS-Based Blood Capillary Sampling for Mass Spectrometry-Based Human Metabolomics Studies |
| title_full | VAMS-Based Blood Capillary Sampling for Mass Spectrometry-Based Human Metabolomics Studies |
| title_fullStr | VAMS-Based Blood Capillary Sampling for Mass Spectrometry-Based Human Metabolomics Studies |
| title_full_unstemmed | VAMS-Based Blood Capillary Sampling for Mass Spectrometry-Based Human Metabolomics Studies |
| title_short | VAMS-Based Blood Capillary Sampling for Mass Spectrometry-Based Human Metabolomics Studies |
| title_sort | vams based blood capillary sampling for mass spectrometry based human metabolomics studies |
| topic | VAMS untargeted metabolomics capillary blood sample collection mass spectrometry |
| url | https://www.mdpi.com/2218-1989/13/2/146 |
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