Initiation of callus and rhizogenesis of Cucumis sativus L. hypocotyl explants

Hypocotyl fragments of Cucumis sativus L. were grown on two basic media: Linsmaier-Skoog's (1964) and White's (1943) modified by Street and McGregor (1952). These media were enriched by addition of some growth regulators (NAA, IAA, 2,4-D) in combination with kinetin. White's basic med...

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Main Authors: B. Wojciechowska, D. Czupryńska, K. Nowak, A. Ciepliński
Format: Article
Language:English
Published: Polish Botanical Society 2013-12-01
Series:Acta Agrobotanica
Online Access:https://pbsociety.org.pl/journals/index.php/aa/article/view/2195
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author B. Wojciechowska
D. Czupryńska
K. Nowak
A. Ciepliński
author_facet B. Wojciechowska
D. Czupryńska
K. Nowak
A. Ciepliński
author_sort B. Wojciechowska
collection DOAJ
description Hypocotyl fragments of Cucumis sativus L. were grown on two basic media: Linsmaier-Skoog's (1964) and White's (1943) modified by Street and McGregor (1952). These media were enriched by addition of some growth regulators (NAA, IAA, 2,4-D) in combination with kinetin. White's basic medium is not suitable for callus culture and for rhisogenesis of cucumber hypocotyls. Linsmaier and Skoog's medium was most suitable for growth of callus. After enrichment of the media with IAA, NAA and 2,4-D differences in the degree of taking root by the explants and callus formation were observed.
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spelling doaj.art-852364f76da94e0eb3a6ee9c9a5b58ea2022-12-21T19:16:47ZengPolish Botanical SocietyActa Agrobotanica2300-357X2013-12-01361-2678110.5586/aa.1983.0071783Initiation of callus and rhizogenesis of Cucumis sativus L. hypocotyl explantsB. Wojciechowska0D. Czupryńska1K. Nowak2A. Ciepliński3Silesian University in KatowiceSilesian University in KatowiceSilesian University in KatowiceSilesian University in KatowiceHypocotyl fragments of Cucumis sativus L. were grown on two basic media: Linsmaier-Skoog's (1964) and White's (1943) modified by Street and McGregor (1952). These media were enriched by addition of some growth regulators (NAA, IAA, 2,4-D) in combination with kinetin. White's basic medium is not suitable for callus culture and for rhisogenesis of cucumber hypocotyls. Linsmaier and Skoog's medium was most suitable for growth of callus. After enrichment of the media with IAA, NAA and 2,4-D differences in the degree of taking root by the explants and callus formation were observed.https://pbsociety.org.pl/journals/index.php/aa/article/view/2195
spellingShingle B. Wojciechowska
D. Czupryńska
K. Nowak
A. Ciepliński
Initiation of callus and rhizogenesis of Cucumis sativus L. hypocotyl explants
Acta Agrobotanica
title Initiation of callus and rhizogenesis of Cucumis sativus L. hypocotyl explants
title_full Initiation of callus and rhizogenesis of Cucumis sativus L. hypocotyl explants
title_fullStr Initiation of callus and rhizogenesis of Cucumis sativus L. hypocotyl explants
title_full_unstemmed Initiation of callus and rhizogenesis of Cucumis sativus L. hypocotyl explants
title_short Initiation of callus and rhizogenesis of Cucumis sativus L. hypocotyl explants
title_sort initiation of callus and rhizogenesis of cucumis sativus l hypocotyl explants
url https://pbsociety.org.pl/journals/index.php/aa/article/view/2195
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