Effect of plant-derived antimicrobials against multidrug-resistant Salmonella Heidelberg in ground Turkey
ABSTRACT: Salmonella Heidelberg (SH) is a highly invasive human pathogen for which turkeys can serve as reservoir hosts. Colonization of turkeys with SH may result in potential contamination and is a greater challenge to prevent in comminuted products. Antimicrobial efficacy of 3 GRAS-status plant-d...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-03-01
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| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579121006027 |
| _version_ | 1818986686862852096 |
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| author | Grace Dewi Shijinaraj Manjankattil Claire Peichel Shiliang Jia Divek Nair Zata Vickers Timothy J. Johnson Carol Cardona Sally Noll Anup Kollanoor Johny |
| author_facet | Grace Dewi Shijinaraj Manjankattil Claire Peichel Shiliang Jia Divek Nair Zata Vickers Timothy J. Johnson Carol Cardona Sally Noll Anup Kollanoor Johny |
| author_sort | Grace Dewi |
| collection | DOAJ |
| description | ABSTRACT: Salmonella Heidelberg (SH) is a highly invasive human pathogen for which turkeys can serve as reservoir hosts. Colonization of turkeys with SH may result in potential contamination and is a greater challenge to prevent in comminuted products. Antimicrobial efficacy of 3 GRAS-status plant-derived antimicrobials (PDAs), lemongrass essential oil (LG), citral (CIT), and trans-cinnamaldehyde (TC), against SH in ground turkey, a comminuted product implicated in several outbreaks, was evaluated in this study. Ground turkey samples inoculated with ∼3.50 log10 CFU/g of a three-strain SH cocktail were treated with either LG, CIT, or TC at either 0.5, 1, or 2% (vol/wt). Samples were stored at 4°C, and bacterial enumeration was performed on d 0, 1, 3, and 5. Appropriate controls were included alongside all treatments. Fluorescence microscopy was performed to evaluate the direct impact of the PDAs against SH in vitro. Appearance and aroma difference testing of raw patties was also performed for select treatments with trained sensory panelists. Treatment with 2% TC yielded a 2.5 log10 CFU/g reduction by d 1 and complete reduction by d 5 (P < 0.05). By d 3, 2% CIT and 2% LG resulted in SH reduction of at least 1.7 log10 CFU/g (P < 0.05). Addition of 1% TC resulted in reduction of at least 1.8 log10 CFU/g by d 3 (P < 0.05). Participants could distinguish PDA-treated raw patties by aroma. Most participants (7/11) could not distinguish patties treated with 0.5% TC based on appearance. Microscopic images indicate that all PDAs resulted in disruption of the SH membrane. Results of the present study indicate that the three tested PDAs, LG, CIT, and TC are effective against SH in ground turkey, indicating their potential use as interventions to mitigate Salmonella contamination in comminuted turkey products. |
| first_indexed | 2024-12-20T18:54:45Z |
| format | Article |
| id | doaj.art-8523695fda104e628b82f8c52aa8bd14 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-12-20T18:54:45Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-8523695fda104e628b82f8c52aa8bd142022-12-21T19:29:33ZengElsevierPoultry Science0032-57912022-03-011013101581Effect of plant-derived antimicrobials against multidrug-resistant Salmonella Heidelberg in ground TurkeyGrace Dewi0Shijinaraj Manjankattil1Claire Peichel2Shiliang Jia3Divek Nair4Zata Vickers5Timothy J. Johnson6Carol Cardona7Sally Noll8Anup Kollanoor Johny9Department of Animal Science, University of Minnesota, Saint Paul, MN 55108, USADepartment of Animal Science, University of Minnesota, Saint Paul, MN 55108, USADepartment of Animal Science, University of Minnesota, Saint Paul, MN 55108, USACollege of Food Science and Engineering, Zhejiang University of Technology, Hangzhou, 310014, ChinaDepartment of Animal Science, University of Minnesota, Saint Paul, MN 55108, USADepartment of Food Science and Nutrition, University of Minnesota, Saint Paul, MN 55108, USADepartment of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, MN 55108, USADepartment of Veterinary and Biomedical Sciences, University of Minnesota, Saint Paul, MN 55108, USADepartment of Animal Science, University of Minnesota, Saint Paul, MN 55108, USADepartment of Animal Science, University of Minnesota, Saint Paul, MN 55108, USA; Corresponding author:ABSTRACT: Salmonella Heidelberg (SH) is a highly invasive human pathogen for which turkeys can serve as reservoir hosts. Colonization of turkeys with SH may result in potential contamination and is a greater challenge to prevent in comminuted products. Antimicrobial efficacy of 3 GRAS-status plant-derived antimicrobials (PDAs), lemongrass essential oil (LG), citral (CIT), and trans-cinnamaldehyde (TC), against SH in ground turkey, a comminuted product implicated in several outbreaks, was evaluated in this study. Ground turkey samples inoculated with ∼3.50 log10 CFU/g of a three-strain SH cocktail were treated with either LG, CIT, or TC at either 0.5, 1, or 2% (vol/wt). Samples were stored at 4°C, and bacterial enumeration was performed on d 0, 1, 3, and 5. Appropriate controls were included alongside all treatments. Fluorescence microscopy was performed to evaluate the direct impact of the PDAs against SH in vitro. Appearance and aroma difference testing of raw patties was also performed for select treatments with trained sensory panelists. Treatment with 2% TC yielded a 2.5 log10 CFU/g reduction by d 1 and complete reduction by d 5 (P < 0.05). By d 3, 2% CIT and 2% LG resulted in SH reduction of at least 1.7 log10 CFU/g (P < 0.05). Addition of 1% TC resulted in reduction of at least 1.8 log10 CFU/g by d 3 (P < 0.05). Participants could distinguish PDA-treated raw patties by aroma. Most participants (7/11) could not distinguish patties treated with 0.5% TC based on appearance. Microscopic images indicate that all PDAs resulted in disruption of the SH membrane. Results of the present study indicate that the three tested PDAs, LG, CIT, and TC are effective against SH in ground turkey, indicating their potential use as interventions to mitigate Salmonella contamination in comminuted turkey products.http://www.sciencedirect.com/science/article/pii/S0032579121006027citrallemongrasstrans-cinnamaldehydeSalmonellaantibacterial |
| spellingShingle | Grace Dewi Shijinaraj Manjankattil Claire Peichel Shiliang Jia Divek Nair Zata Vickers Timothy J. Johnson Carol Cardona Sally Noll Anup Kollanoor Johny Effect of plant-derived antimicrobials against multidrug-resistant Salmonella Heidelberg in ground Turkey Poultry Science citral lemongrass trans-cinnamaldehyde Salmonella antibacterial |
| title | Effect of plant-derived antimicrobials against multidrug-resistant Salmonella Heidelberg in ground Turkey |
| title_full | Effect of plant-derived antimicrobials against multidrug-resistant Salmonella Heidelberg in ground Turkey |
| title_fullStr | Effect of plant-derived antimicrobials against multidrug-resistant Salmonella Heidelberg in ground Turkey |
| title_full_unstemmed | Effect of plant-derived antimicrobials against multidrug-resistant Salmonella Heidelberg in ground Turkey |
| title_short | Effect of plant-derived antimicrobials against multidrug-resistant Salmonella Heidelberg in ground Turkey |
| title_sort | effect of plant derived antimicrobials against multidrug resistant salmonella heidelberg in ground turkey |
| topic | citral lemongrass trans-cinnamaldehyde Salmonella antibacterial |
| url | http://www.sciencedirect.com/science/article/pii/S0032579121006027 |
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