An effective UFLC–MS/MS method used to study pharmacokinetics of major constituents of Fukeqianjin formula in rat plasma
Abstract Background Fukeqianjin formula (FKQJF) is a Chinese medicine prescription, which has been widely used individually or in combination with other western medicine for the treatment of various gynecological inflammatory diseases, including chronic cervicitis, chronic pelvic inflammatory diseas...
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| Format: | Article |
| Language: | English |
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BMC
2020-07-01
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| Series: | Chinese Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s13020-020-00347-5 |
| _version_ | 1819231721086779392 |
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| author | Kanghua Wang Lu Liu Yanfang Yang Xiaoyan Liu Lei Zhang Wei Xu Yingtao Zhang Xiuwei Yang Peng Zhang Kaifeng Peng Yun Gong Nifu Liu |
| author_facet | Kanghua Wang Lu Liu Yanfang Yang Xiaoyan Liu Lei Zhang Wei Xu Yingtao Zhang Xiuwei Yang Peng Zhang Kaifeng Peng Yun Gong Nifu Liu |
| author_sort | Kanghua Wang |
| collection | DOAJ |
| description | Abstract Background Fukeqianjin formula (FKQJF) is a Chinese medicine prescription, which has been widely used individually or in combination with other western medicine for the treatment of various gynecological inflammatory diseases, including chronic cervicitis, chronic pelvic inflammatory disease and endometritis, so on and so force. Methods The ultra-fast liquid chromatography coupled with triple quadrupole tandem mass spectrometry (UFLC–MS/MS), a quick and efficient method was established and applied to quantify the major constituents of Fukeqianjin formula in rat plasma, and its pharmacokinetics of oral absorption was studied. Nineteen components in Fukeqianjin formula were detected and identified as the major compounds absorbed into the blood according to their chromatographic behavior, molecular weight, ion fragments and other information of these compounds. Furthermore, the plasma drug concentration–time curves were established and the related kinetic parameters were analyzed. Results The results showed that all the 19 compounds could be rapidly absorbed by the gastrointestinal tract, the plasma drug concentration of most compounds could reach a peak at around 1–2 h, and the double-peaks on behalf of the enterohepatic circulation were found in most drug concentration–time curves. The method used in this experiment was validated comprehensively including specificity, linearity, precision, accuracy, stability, matrix effect, and recovery. Conclusions These results showed that the developed method was suitable for pharmacokinetic analysis of the main components of Fukeqianjin formula in rat plasma, and may provide useful information for the subsequent distribution studies in vivo. |
| first_indexed | 2024-12-23T11:49:27Z |
| format | Article |
| id | doaj.art-852b078917634bc7a828248e7ac1b183 |
| institution | Directory Open Access Journal |
| issn | 1749-8546 |
| language | English |
| last_indexed | 2024-12-23T11:49:27Z |
| publishDate | 2020-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Chinese Medicine |
| spelling | doaj.art-852b078917634bc7a828248e7ac1b1832022-12-21T17:48:14ZengBMCChinese Medicine1749-85462020-07-0115111510.1186/s13020-020-00347-5An effective UFLC–MS/MS method used to study pharmacokinetics of major constituents of Fukeqianjin formula in rat plasmaKanghua Wang0Lu Liu1Yanfang Yang2Xiaoyan Liu3Lei Zhang4Wei Xu5Yingtao Zhang6Xiuwei Yang7Peng Zhang8Kaifeng Peng9Yun Gong10Nifu Liu11State Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Peking UniversityState Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Peking UniversityState Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Peking UniversityState Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Peking UniversityState Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Peking UniversityState Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Peking UniversityState Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Peking UniversityState Key Laboratory of Natural and Biomimetic Drugs, Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University Health Science Center, Peking UniversityZhuzhou Qianjin Pharmaceutical Co., LtdZhuzhou Qianjin Pharmaceutical Co., LtdZhuzhou Qianjin Pharmaceutical Co., LtdZhuzhou Qianjin Pharmaceutical Co., LtdAbstract Background Fukeqianjin formula (FKQJF) is a Chinese medicine prescription, which has been widely used individually or in combination with other western medicine for the treatment of various gynecological inflammatory diseases, including chronic cervicitis, chronic pelvic inflammatory disease and endometritis, so on and so force. Methods The ultra-fast liquid chromatography coupled with triple quadrupole tandem mass spectrometry (UFLC–MS/MS), a quick and efficient method was established and applied to quantify the major constituents of Fukeqianjin formula in rat plasma, and its pharmacokinetics of oral absorption was studied. Nineteen components in Fukeqianjin formula were detected and identified as the major compounds absorbed into the blood according to their chromatographic behavior, molecular weight, ion fragments and other information of these compounds. Furthermore, the plasma drug concentration–time curves were established and the related kinetic parameters were analyzed. Results The results showed that all the 19 compounds could be rapidly absorbed by the gastrointestinal tract, the plasma drug concentration of most compounds could reach a peak at around 1–2 h, and the double-peaks on behalf of the enterohepatic circulation were found in most drug concentration–time curves. The method used in this experiment was validated comprehensively including specificity, linearity, precision, accuracy, stability, matrix effect, and recovery. Conclusions These results showed that the developed method was suitable for pharmacokinetic analysis of the main components of Fukeqianjin formula in rat plasma, and may provide useful information for the subsequent distribution studies in vivo.http://link.springer.com/article/10.1186/s13020-020-00347-5Fukeqianjin formulaPharmacokineticsUFLC–MS/MS |
| spellingShingle | Kanghua Wang Lu Liu Yanfang Yang Xiaoyan Liu Lei Zhang Wei Xu Yingtao Zhang Xiuwei Yang Peng Zhang Kaifeng Peng Yun Gong Nifu Liu An effective UFLC–MS/MS method used to study pharmacokinetics of major constituents of Fukeqianjin formula in rat plasma Chinese Medicine Fukeqianjin formula Pharmacokinetics UFLC–MS/MS |
| title | An effective UFLC–MS/MS method used to study pharmacokinetics of major constituents of Fukeqianjin formula in rat plasma |
| title_full | An effective UFLC–MS/MS method used to study pharmacokinetics of major constituents of Fukeqianjin formula in rat plasma |
| title_fullStr | An effective UFLC–MS/MS method used to study pharmacokinetics of major constituents of Fukeqianjin formula in rat plasma |
| title_full_unstemmed | An effective UFLC–MS/MS method used to study pharmacokinetics of major constituents of Fukeqianjin formula in rat plasma |
| title_short | An effective UFLC–MS/MS method used to study pharmacokinetics of major constituents of Fukeqianjin formula in rat plasma |
| title_sort | effective uflc ms ms method used to study pharmacokinetics of major constituents of fukeqianjin formula in rat plasma |
| topic | Fukeqianjin formula Pharmacokinetics UFLC–MS/MS |
| url | http://link.springer.com/article/10.1186/s13020-020-00347-5 |
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