A poly (glycerol-sebacate-acrylate) nanosphere enhanced injectable hydrogel for wound treatment
Wound repair remains a huge clinical challenge, which can cause bleeding, infection, and patient death. In our current research, a bioactive, injectable, multifunctional composite hydrogel doped with nanospheres was prepared with antibacterial and angiogenesis-promoting functions for the treatment o...
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1091122/full |
| _version_ | 1797955359733710848 |
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| author | Jiajia Luo Fenglei Sun Pinhua Rao Tonghe Zhu Yonghang Liu Juan Du Sihao Chen Xiangyun Jin Jiale Jin Yi Chai |
| author_facet | Jiajia Luo Fenglei Sun Pinhua Rao Tonghe Zhu Yonghang Liu Juan Du Sihao Chen Xiangyun Jin Jiale Jin Yi Chai |
| author_sort | Jiajia Luo |
| collection | DOAJ |
| description | Wound repair remains a huge clinical challenge, which can cause bleeding, infection, and patient death. In our current research, a bioactive, injectable, multifunctional composite hydrogel doped with nanospheres was prepared with antibacterial and angiogenesis-promoting functions for the treatment of wounds. Amino groups in ε-polylysine (ε-EPL) undergo dynamic Schiff base reaction cross-linking with oxidized hyaluronic acid (OHA), and F127 exhibits unique temperature sensitivity to form an injectable thermosensitive hydrogel (FHE10), which can form a hydrogel to cover the wound at body temperature. Nanospheres (PNs) prepared using poly (glyceryl-sebacate-acrylate) (PGSA) were loaded into hydrogels (FHE10) for promoting wound repair. The prepared FHE10 exhibited rapid gelation, good injectable abilities, and showed resistance to the flourish of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). In vitro investigations showed that FHE10 had good hemocompatibility and cytocompatibility. FHE10@PNs exhibited good proliferation, migration, and tube formation of human umbilical vein endothelial cells (HUVECs) and human foreskin fibroblasts (HFF-1). Furthermore, FHE10@PNs significantly promoted reepithelialization and collagen deposition as well as micro-vascularization compared with the use of FHE10 or PNs alone, thereby accelerating the repair of wounds. In general, this study demonstrated that the multifunctional injectable composite hydrogel showed great potential in wound treatment. |
| first_indexed | 2024-04-10T23:32:58Z |
| format | Article |
| id | doaj.art-852b3ecc244b434e9a9f60b98e0d92e5 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-10T23:32:58Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-852b3ecc244b434e9a9f60b98e0d92e52023-01-12T04:35:45ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-01-011010.3389/fbioe.2022.10911221091122A poly (glycerol-sebacate-acrylate) nanosphere enhanced injectable hydrogel for wound treatmentJiajia Luo0Fenglei Sun1Pinhua Rao2Tonghe Zhu3Yonghang Liu4Juan Du5Sihao Chen6Xiangyun Jin7Jiale Jin8Yi Chai9School of Chemistry and Chemical Engineering, Institute for Frontier Medical Technology, Shanghai Engineering Research Center of Pharmaceutical Intelligent Equipment, Shanghai Frontiers Science Research Center for Druggability of Cardiovascular Non-coding RNA, Shanghai University of Engineering Science, Shanghai, ChinaDepartment of Neurosurgery, Weifang People’s Hospital, Weifang, Shandong, ChinaSchool of Chemistry and Chemical Engineering, Institute for Frontier Medical Technology, Shanghai Engineering Research Center of Pharmaceutical Intelligent Equipment, Shanghai Frontiers Science Research Center for Druggability of Cardiovascular Non-coding RNA, Shanghai University of Engineering Science, Shanghai, ChinaSchool of Chemistry and Chemical Engineering, Institute for Frontier Medical Technology, Shanghai Engineering Research Center of Pharmaceutical Intelligent Equipment, Shanghai Frontiers Science Research Center for Druggability of Cardiovascular Non-coding RNA, Shanghai University of Engineering Science, Shanghai, ChinaSchool of Chemistry and Chemical Engineering, Institute for Frontier Medical Technology, Shanghai Engineering Research Center of Pharmaceutical Intelligent Equipment, Shanghai Frontiers Science Research Center for Druggability of Cardiovascular Non-coding RNA, Shanghai University of Engineering Science, Shanghai, ChinaSchool of Chemistry and Chemical Engineering, Institute for Frontier Medical Technology, Shanghai Engineering Research Center of Pharmaceutical Intelligent Equipment, Shanghai Frontiers Science Research Center for Druggability of Cardiovascular Non-coding RNA, Shanghai University of Engineering Science, Shanghai, ChinaSchool of Chemistry and Chemical Engineering, Institute for Frontier Medical Technology, Shanghai Engineering Research Center of Pharmaceutical Intelligent Equipment, Shanghai Frontiers Science Research Center for Druggability of Cardiovascular Non-coding RNA, Shanghai University of Engineering Science, Shanghai, ChinaDepartment of Orthopaedics, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaSpine Lab, Department of Orthopaedic Surgery, The First Affiliated Hospital, Zhejiang University, Hangzhou, ChinaDepartment of Neurosurgery, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaWound repair remains a huge clinical challenge, which can cause bleeding, infection, and patient death. In our current research, a bioactive, injectable, multifunctional composite hydrogel doped with nanospheres was prepared with antibacterial and angiogenesis-promoting functions for the treatment of wounds. Amino groups in ε-polylysine (ε-EPL) undergo dynamic Schiff base reaction cross-linking with oxidized hyaluronic acid (OHA), and F127 exhibits unique temperature sensitivity to form an injectable thermosensitive hydrogel (FHE10), which can form a hydrogel to cover the wound at body temperature. Nanospheres (PNs) prepared using poly (glyceryl-sebacate-acrylate) (PGSA) were loaded into hydrogels (FHE10) for promoting wound repair. The prepared FHE10 exhibited rapid gelation, good injectable abilities, and showed resistance to the flourish of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). In vitro investigations showed that FHE10 had good hemocompatibility and cytocompatibility. FHE10@PNs exhibited good proliferation, migration, and tube formation of human umbilical vein endothelial cells (HUVECs) and human foreskin fibroblasts (HFF-1). Furthermore, FHE10@PNs significantly promoted reepithelialization and collagen deposition as well as micro-vascularization compared with the use of FHE10 or PNs alone, thereby accelerating the repair of wounds. In general, this study demonstrated that the multifunctional injectable composite hydrogel showed great potential in wound treatment.https://www.frontiersin.org/articles/10.3389/fbioe.2022.1091122/fullpoly (glycerol-sebacate-acrylate) nanosphereinjectable hydrogelantibacterialvascularizationwound treatment |
| spellingShingle | Jiajia Luo Fenglei Sun Pinhua Rao Tonghe Zhu Yonghang Liu Juan Du Sihao Chen Xiangyun Jin Jiale Jin Yi Chai A poly (glycerol-sebacate-acrylate) nanosphere enhanced injectable hydrogel for wound treatment Frontiers in Bioengineering and Biotechnology poly (glycerol-sebacate-acrylate) nanosphere injectable hydrogel antibacterial vascularization wound treatment |
| title | A poly (glycerol-sebacate-acrylate) nanosphere enhanced injectable hydrogel for wound treatment |
| title_full | A poly (glycerol-sebacate-acrylate) nanosphere enhanced injectable hydrogel for wound treatment |
| title_fullStr | A poly (glycerol-sebacate-acrylate) nanosphere enhanced injectable hydrogel for wound treatment |
| title_full_unstemmed | A poly (glycerol-sebacate-acrylate) nanosphere enhanced injectable hydrogel for wound treatment |
| title_short | A poly (glycerol-sebacate-acrylate) nanosphere enhanced injectable hydrogel for wound treatment |
| title_sort | poly glycerol sebacate acrylate nanosphere enhanced injectable hydrogel for wound treatment |
| topic | poly (glycerol-sebacate-acrylate) nanosphere injectable hydrogel antibacterial vascularization wound treatment |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.1091122/full |
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