The Effects of Rosmarinic Acid on the Liver Fibrosis Induced by Non-alcoholic Steatohepatitis in Male Mice
Background and Objectives: Non-Alcoholic Steatohepatitis (NASH) is a serious and increasing liver disease, which develops into cirrhosis, fibrosis, and hepatocellular carcinoma. Rosmarinic Acid (RA) is a powerful antioxidant and anti-inflammatory compound. Therefore, this study aimed to assess the r...
| Main Authors: | , |
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| Format: | Article |
| Language: | fas |
| Published: |
Qom University of Medical Sciences
2021-03-01
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| Series: | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum |
| Subjects: | |
| Online Access: | http://journal.muq.ac.ir/article-1-3091-en.html |
| Summary: | Background and Objectives: Non-Alcoholic Steatohepatitis (NASH) is a serious and increasing liver disease, which develops into cirrhosis, fibrosis, and hepatocellular carcinoma. Rosmarinic Acid (RA) is a powerful antioxidant and anti-inflammatory compound. Therefore, this study aimed to assess the role of RA on a mouse model of NASH-induced liver fibrosis.
Methods In this research, C57/BL6 mice were divided into 4 equal groups, as follows: the control, RA, NASH, and NASH+RA groups. In the third and fourth groups, NASH was induced by Methionine-Choline Deficient (MCD) diet for 8 weeks. In the RA and NASH+RA group, RA was intraperitoneally injected daily for 8 weeks in mice. After 8 weeks, the explored animals were sacrificed and the blood and liver samples were obtained. Then, Trichrome-Masson staining, serum, and molecular analyses were performed.
Results After RA administration, the plasma levels of ALT and AST decreased. RA administration also reduced TGFβ and α-SMA genes expression. Finally, the histopathological evaluation revealed that RA administration decreased NASH-induced liver fibrosis.
Conclusion It seems that RA administration improves NASH-induced liver fibrosis through the inhibition of the α-SMA/ TGF-β signaling pathway. |
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| ISSN: | 1735-7799 2008-1375 |