Do age and mating status affect olfactory response of the parasitoid, Microplitis croceipes (Hymenoptera: Braconidae) to host-related plant odors? [version 1; referees: 2 approved]
Background: Parasitic wasps (parasitoids) use volatile organic compounds released by herbivore-infested plants to locate their hosts. Response of parasitoids to plant odors may be plastic and dependent on their physiological state. Using Microplitis croceipes (Hymenoptera: Braconidae), a relatively...
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| Format: | Article |
| Language: | English |
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F1000 Research Ltd
2018-11-01
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| Series: | F1000Research |
| Online Access: | https://f1000research.com/articles/7-1817/v1 |
| _version_ | 1818913138733481984 |
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| author | Matthew Burrows Tolulope Morawo Henry Fadamiro |
| author_facet | Matthew Burrows Tolulope Morawo Henry Fadamiro |
| author_sort | Matthew Burrows |
| collection | DOAJ |
| description | Background: Parasitic wasps (parasitoids) use volatile organic compounds released by herbivore-infested plants to locate their hosts. Response of parasitoids to plant odors may be plastic and dependent on their physiological state. Using Microplitis croceipes (Hymenoptera: Braconidae), a relatively specialized larval endoparasitoid of Heliothis virescens (Lepidoptera: Noctuidae), we asked whether age and mating status of parasitoids affect their olfactory response to host-related odors. Methods: Four odor stimuli of varying complexity were selected based on previous reports of parasitoid response to cotton volatiles: cis-3-hexenol (a green leaf volatile), α-pinene (a constitutive monoterpene), a 50/50 v/v binary mixture (cis-3-hexenol + α-pinene), and H. virescens-infested cotton odors. Female M. croceipes used in Y-tube olfactometer bioassays were either mated or unmated, and grouped 1–3, 4–6, and 7–9 d-old. Female parasitoids used in electroantennogram (EAG) recording were mated and grouped 1–3, 4–6, 7–9 and 10–12 d-old. Results: In Y-tube olfactometer bioassays, neither age nor mating status played a major role in the attraction of parasitoids to test odor stimuli, with two exceptions: 4–6 d-old mated parasitoids showed attraction to the binary mixture, and 1–3 d-old mated parasitoids showed attraction to H. virescens-infested cotton. Age did not affect EAG response of parasitoids to test stimuli. Conclusions: The present results suggest that age and mating status do not play a major role in modulating olfactory responses of M. croceipes to host-related plant odors. Instead, plasticity of olfactory response may be limited in M. croceipes due to strong innate sensitivity to host-related odor cues. |
| first_indexed | 2024-12-19T23:25:44Z |
| format | Article |
| id | doaj.art-854275a88304455785a9c18dbb40cc80 |
| institution | Directory Open Access Journal |
| issn | 2046-1402 |
| language | English |
| last_indexed | 2024-12-19T23:25:44Z |
| publishDate | 2018-11-01 |
| publisher | F1000 Research Ltd |
| record_format | Article |
| series | F1000Research |
| spelling | doaj.art-854275a88304455785a9c18dbb40cc802022-12-21T20:01:51ZengF1000 Research LtdF1000Research2046-14022018-11-01710.12688/f1000research.16927.118508Do age and mating status affect olfactory response of the parasitoid, Microplitis croceipes (Hymenoptera: Braconidae) to host-related plant odors? [version 1; referees: 2 approved]Matthew Burrows0Tolulope Morawo1Henry Fadamiro2Entomology and Plant Pathology, Auburn University, Auburn, Alabama, 36849, USAEntomology and Plant Pathology, Auburn University, Auburn, Alabama, 36849, USAEntomology and Plant Pathology, Auburn University, Auburn, Alabama, 36849, USABackground: Parasitic wasps (parasitoids) use volatile organic compounds released by herbivore-infested plants to locate their hosts. Response of parasitoids to plant odors may be plastic and dependent on their physiological state. Using Microplitis croceipes (Hymenoptera: Braconidae), a relatively specialized larval endoparasitoid of Heliothis virescens (Lepidoptera: Noctuidae), we asked whether age and mating status of parasitoids affect their olfactory response to host-related odors. Methods: Four odor stimuli of varying complexity were selected based on previous reports of parasitoid response to cotton volatiles: cis-3-hexenol (a green leaf volatile), α-pinene (a constitutive monoterpene), a 50/50 v/v binary mixture (cis-3-hexenol + α-pinene), and H. virescens-infested cotton odors. Female M. croceipes used in Y-tube olfactometer bioassays were either mated or unmated, and grouped 1–3, 4–6, and 7–9 d-old. Female parasitoids used in electroantennogram (EAG) recording were mated and grouped 1–3, 4–6, 7–9 and 10–12 d-old. Results: In Y-tube olfactometer bioassays, neither age nor mating status played a major role in the attraction of parasitoids to test odor stimuli, with two exceptions: 4–6 d-old mated parasitoids showed attraction to the binary mixture, and 1–3 d-old mated parasitoids showed attraction to H. virescens-infested cotton. Age did not affect EAG response of parasitoids to test stimuli. Conclusions: The present results suggest that age and mating status do not play a major role in modulating olfactory responses of M. croceipes to host-related plant odors. Instead, plasticity of olfactory response may be limited in M. croceipes due to strong innate sensitivity to host-related odor cues.https://f1000research.com/articles/7-1817/v1 |
| spellingShingle | Matthew Burrows Tolulope Morawo Henry Fadamiro Do age and mating status affect olfactory response of the parasitoid, Microplitis croceipes (Hymenoptera: Braconidae) to host-related plant odors? [version 1; referees: 2 approved] F1000Research |
| title | Do age and mating status affect olfactory response of the parasitoid, Microplitis croceipes (Hymenoptera: Braconidae) to host-related plant odors? [version 1; referees: 2 approved] |
| title_full | Do age and mating status affect olfactory response of the parasitoid, Microplitis croceipes (Hymenoptera: Braconidae) to host-related plant odors? [version 1; referees: 2 approved] |
| title_fullStr | Do age and mating status affect olfactory response of the parasitoid, Microplitis croceipes (Hymenoptera: Braconidae) to host-related plant odors? [version 1; referees: 2 approved] |
| title_full_unstemmed | Do age and mating status affect olfactory response of the parasitoid, Microplitis croceipes (Hymenoptera: Braconidae) to host-related plant odors? [version 1; referees: 2 approved] |
| title_short | Do age and mating status affect olfactory response of the parasitoid, Microplitis croceipes (Hymenoptera: Braconidae) to host-related plant odors? [version 1; referees: 2 approved] |
| title_sort | do age and mating status affect olfactory response of the parasitoid microplitis croceipes hymenoptera braconidae to host related plant odors version 1 referees 2 approved |
| url | https://f1000research.com/articles/7-1817/v1 |
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