Complanatuside alleviates inflammatory cell damage induced by pro-inflammatory cytokines in skin keratinocytes
Cytokine-mediated inflammatory response is considered a cause of skin lesion in COVID-19 patients. Complanatuside is a flavonol glycoside isolated from Astragalus complanatus. Flavonoids from Astragalus complanatus were reported to have anti-inflammatory and anticancer activities but the potential p...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2022.909651/full |
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| author | Xiao Wang Xuetao Xu Xuetao Xu Panpan Wu Panpan Wu Mengshuo Wu Lishe Gan Lishe Gan Jingwei Jin Rihui Wu Wenfeng Liu Kun Zhang Dongli Li Xi Zheng |
| author_facet | Xiao Wang Xuetao Xu Xuetao Xu Panpan Wu Panpan Wu Mengshuo Wu Lishe Gan Lishe Gan Jingwei Jin Rihui Wu Wenfeng Liu Kun Zhang Dongli Li Xi Zheng |
| author_sort | Xiao Wang |
| collection | DOAJ |
| description | Cytokine-mediated inflammatory response is considered a cause of skin lesion in COVID-19 patients. Complanatuside is a flavonol glycoside isolated from Astragalus complanatus. Flavonoids from Astragalus complanatus were reported to have anti-inflammatory and anticancer activities but the potential protective effect of complanatuside on cytokine-induced inflammatory damage in skin keratinocytes is not known. The aim of this study is to explore the inhibitory effect of complanatuside on inflammation and its underlying mechanisms in skin epithelial HaCaT cells treated with inflammatory cytokines. The combination of IFN-γ, TNF-α, and IL-6 decreased cell viability, increased cell death, and pyroptosis in HaCaT cells. Treatment with complanatuside alleviated the effects of the cytokine combination on HaCaT cells. Complanatuside down-regulated pyroptosis related to NLRP3, GSDMD, and ASC. The effects of complanatuside were related to vast reductions in the levels of iNOS, COX-2, and ROS. Results of the present study indicate that complanatuside inhibited inflammation and protected the cells from inflammatory cell damage in HaCaT cells treated with the combination of IFN-γ, TNF-α, and IL-6. Complanatuside may be a promising candidate for inhibiting COVID-19 related skin inflammatory damage. |
| first_indexed | 2024-12-10T20:19:15Z |
| format | Article |
| id | doaj.art-8543e0f9fac846a8bb9d21142f82f5c2 |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-12-10T20:19:15Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-8543e0f9fac846a8bb9d21142f82f5c22022-12-22T01:35:06ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462022-08-011010.3389/fchem.2022.909651909651Complanatuside alleviates inflammatory cell damage induced by pro-inflammatory cytokines in skin keratinocytesXiao Wang0Xuetao Xu1Xuetao Xu2Panpan Wu3Panpan Wu4Mengshuo Wu5Lishe Gan6Lishe Gan7Jingwei Jin8Rihui Wu9Wenfeng Liu10Kun Zhang11Dongli Li12Xi Zheng13School of Biotechnology and Health Sciences, Wuyi University, Jiangmen City, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen City, ChinaInternational Healthcare Innovation Institute (Jiangmen), Jiangmen, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen City, ChinaInternational Healthcare Innovation Institute (Jiangmen), Jiangmen, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen City, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen City, ChinaInternational Healthcare Innovation Institute (Jiangmen), Jiangmen, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen City, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen City, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen City, ChinaSchool of Biotechnology and Health Sciences, Wuyi University, Jiangmen City, ChinaInternational Healthcare Innovation Institute (Jiangmen), Jiangmen, ChinaDepartment of Chemical Biology, Ernest Mario School of Pharmacy, Rutgers, the State University of New Jersey, Piscataway, NJ, United StatesCytokine-mediated inflammatory response is considered a cause of skin lesion in COVID-19 patients. Complanatuside is a flavonol glycoside isolated from Astragalus complanatus. Flavonoids from Astragalus complanatus were reported to have anti-inflammatory and anticancer activities but the potential protective effect of complanatuside on cytokine-induced inflammatory damage in skin keratinocytes is not known. The aim of this study is to explore the inhibitory effect of complanatuside on inflammation and its underlying mechanisms in skin epithelial HaCaT cells treated with inflammatory cytokines. The combination of IFN-γ, TNF-α, and IL-6 decreased cell viability, increased cell death, and pyroptosis in HaCaT cells. Treatment with complanatuside alleviated the effects of the cytokine combination on HaCaT cells. Complanatuside down-regulated pyroptosis related to NLRP3, GSDMD, and ASC. The effects of complanatuside were related to vast reductions in the levels of iNOS, COX-2, and ROS. Results of the present study indicate that complanatuside inhibited inflammation and protected the cells from inflammatory cell damage in HaCaT cells treated with the combination of IFN-γ, TNF-α, and IL-6. Complanatuside may be a promising candidate for inhibiting COVID-19 related skin inflammatory damage.https://www.frontiersin.org/articles/10.3389/fchem.2022.909651/fullHaCaT cellsskin inflammatory damagecomplanatusidepyroptosisCOVID-19 |
| spellingShingle | Xiao Wang Xuetao Xu Xuetao Xu Panpan Wu Panpan Wu Mengshuo Wu Lishe Gan Lishe Gan Jingwei Jin Rihui Wu Wenfeng Liu Kun Zhang Dongli Li Xi Zheng Complanatuside alleviates inflammatory cell damage induced by pro-inflammatory cytokines in skin keratinocytes Frontiers in Chemistry HaCaT cells skin inflammatory damage complanatuside pyroptosis COVID-19 |
| title | Complanatuside alleviates inflammatory cell damage induced by pro-inflammatory cytokines in skin keratinocytes |
| title_full | Complanatuside alleviates inflammatory cell damage induced by pro-inflammatory cytokines in skin keratinocytes |
| title_fullStr | Complanatuside alleviates inflammatory cell damage induced by pro-inflammatory cytokines in skin keratinocytes |
| title_full_unstemmed | Complanatuside alleviates inflammatory cell damage induced by pro-inflammatory cytokines in skin keratinocytes |
| title_short | Complanatuside alleviates inflammatory cell damage induced by pro-inflammatory cytokines in skin keratinocytes |
| title_sort | complanatuside alleviates inflammatory cell damage induced by pro inflammatory cytokines in skin keratinocytes |
| topic | HaCaT cells skin inflammatory damage complanatuside pyroptosis COVID-19 |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2022.909651/full |
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