Chemical Characterization, Leishmanicidal Activity and In Vitro Cytotoxicity of the Essential Oil Extracted from <i>Pectis brevipedunculata</i> (Gardner) Sch.Bip. and Its Incorporation into Microemulsion Systems
<i>Pectis brevipedunculata</i> (Gardner) Sch.Bip., known in Brazil as alecrim do campo, is a small Asteraceae family plant with a calming effect and consumed as tea. This species contains components, such as neral and geranial, that display various biological activities, such as leishman...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/16/1/87 |
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| author | Auxiliadora Cristina Correa Barata Lopes Jessyane Rodrigues do Nascimento Marcos Bispo Pinheiro Camara Aldilene da Silva Lima Gláucia Laís Nunes Lopes Matheus Oliveira do Nascimento Júlia Karla Albuquerque Melo Xavier Caroline Martins de Jesus Cáritas de Jesus Silva Mendonça André Luis Menezes Carvalho Lucilene Amorim Silva Cláudia Quintino da Rocha |
| author_facet | Auxiliadora Cristina Correa Barata Lopes Jessyane Rodrigues do Nascimento Marcos Bispo Pinheiro Camara Aldilene da Silva Lima Gláucia Laís Nunes Lopes Matheus Oliveira do Nascimento Júlia Karla Albuquerque Melo Xavier Caroline Martins de Jesus Cáritas de Jesus Silva Mendonça André Luis Menezes Carvalho Lucilene Amorim Silva Cláudia Quintino da Rocha |
| author_sort | Auxiliadora Cristina Correa Barata Lopes |
| collection | DOAJ |
| description | <i>Pectis brevipedunculata</i> (Gardner) Sch.Bip., known in Brazil as alecrim do campo, is a small Asteraceae family plant with a calming effect and consumed as tea. This species contains components, such as neral and geranial, that display various biological activities, such as leishmanicidal. The aim was to chemically characterize the essential oil (EO) obtained from <i>P. brevipedunculata</i> (EO-PB) by hydrodistillation and a microemulsion formulated with EO (ME-PB), Tween 80 and Transcutol P, assess the leishmanicidal effect against <i>Leishmania</i> (L.) <i>amazonensis</i> promastigotes and cytotoxicity against RAW 264.7. EO-PB and ME-PB were analyzed by Gas Chromatography Mass Spectrometry (GC/MS). Monoterpene hydrocarbons were noteworthy among the identified compounds. The main EO-PB constituents were α-pinene and limonene, followed by neral and geranial, which were maintained in ME-PB. EO-PB presented an inhibitory concentration (IC<sub>50</sub>) of 20 µg/mL and ME-PB of 0.93 µg/mL. ME-PB inhibition towards the parasite was 20-fold higher than that of EO-PB. This indicated that EO incorporation to the microemulsion resulted in optimized biological activity. Selectivity indices indicate that ME-PB is more selective concerning parasite inhibition. Thus, ME-PB may comprise an adequate approach against Leishmania, as the inhibitory concentration (IC<sub>50</sub>) promastigotes was lower than that considered toxic for cells cell cytotoxicity of 50% (CC<sub>50</sub>). |
| first_indexed | 2024-03-08T10:37:35Z |
| format | Article |
| id | doaj.art-8543e56c41874be5bccc230e1403aa29 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-08T10:37:35Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-8543e56c41874be5bccc230e1403aa292024-01-26T18:07:10ZengMDPI AGPharmaceutics1999-49232024-01-011618710.3390/pharmaceutics16010087Chemical Characterization, Leishmanicidal Activity and In Vitro Cytotoxicity of the Essential Oil Extracted from <i>Pectis brevipedunculata</i> (Gardner) Sch.Bip. and Its Incorporation into Microemulsion SystemsAuxiliadora Cristina Correa Barata Lopes0Jessyane Rodrigues do Nascimento1Marcos Bispo Pinheiro Camara2Aldilene da Silva Lima3Gláucia Laís Nunes Lopes4Matheus Oliveira do Nascimento5Júlia Karla Albuquerque Melo Xavier6Caroline Martins de Jesus7Cáritas de Jesus Silva Mendonça8André Luis Menezes Carvalho9Lucilene Amorim Silva10Cláudia Quintino da Rocha11Programa de Pós-Graduação em Biodiversidade e Biotecnologia da Rede BIONORTE (PPG-BIONORTE), Universidade Federal do Maranhão, São Luís 65080-805, MA, BrazilPrograma de Pós-Graduação em Química, Universidade Estadual Paulista Júlio de Mesquita Filho, Araraquara 14800-060, SP, BrazilPrograma de Pós-Graduação em Biodiversidade e Biotecnologia da Rede BIONORTE (PPG-BIONORTE), Universidade Federal do Maranhão, São Luís 65080-805, MA, BrazilPrograma de Pós-Graduação em Química, Universidade Federal do Maranhão, São Luís 65080-805, MA, BrazilPrograma de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Piauí, Teresina 64049-550, PI, BrazilPrograma de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Piauí, Teresina 64049-550, PI, BrazilPrograma de Pós-Graduação em Química, Universidade Federal do Maranhão, São Luís 65080-805, MA, BrazilPrograma de Pós-Graduação em Saúde e Tecnologia, Universidade Federal do Maranhão, Imperatriz 65900-410, MA, BrazilPrograma de Pós-Graduação em Química, Universidade Federal do Maranhão, São Luís 65080-805, MA, BrazilPrograma de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Piauí, Teresina 64049-550, PI, BrazilPrograma de Pós-Graduação em Ciências da Saúde, Universidade Federal do Maranhão, São Luís 65080-805, MA, BrazilPrograma de Pós-Graduação em Biodiversidade e Biotecnologia da Rede BIONORTE (PPG-BIONORTE), Universidade Federal do Maranhão, São Luís 65080-805, MA, Brazil<i>Pectis brevipedunculata</i> (Gardner) Sch.Bip., known in Brazil as alecrim do campo, is a small Asteraceae family plant with a calming effect and consumed as tea. This species contains components, such as neral and geranial, that display various biological activities, such as leishmanicidal. The aim was to chemically characterize the essential oil (EO) obtained from <i>P. brevipedunculata</i> (EO-PB) by hydrodistillation and a microemulsion formulated with EO (ME-PB), Tween 80 and Transcutol P, assess the leishmanicidal effect against <i>Leishmania</i> (L.) <i>amazonensis</i> promastigotes and cytotoxicity against RAW 264.7. EO-PB and ME-PB were analyzed by Gas Chromatography Mass Spectrometry (GC/MS). Monoterpene hydrocarbons were noteworthy among the identified compounds. The main EO-PB constituents were α-pinene and limonene, followed by neral and geranial, which were maintained in ME-PB. EO-PB presented an inhibitory concentration (IC<sub>50</sub>) of 20 µg/mL and ME-PB of 0.93 µg/mL. ME-PB inhibition towards the parasite was 20-fold higher than that of EO-PB. This indicated that EO incorporation to the microemulsion resulted in optimized biological activity. Selectivity indices indicate that ME-PB is more selective concerning parasite inhibition. Thus, ME-PB may comprise an adequate approach against Leishmania, as the inhibitory concentration (IC<sub>50</sub>) promastigotes was lower than that considered toxic for cells cell cytotoxicity of 50% (CC<sub>50</sub>).https://www.mdpi.com/1999-4923/16/1/87Asteraceaeformulation<i>L.</i> (L.) <i>amazonensis</i>monoterpenesnanotechnologypromastigote |
| spellingShingle | Auxiliadora Cristina Correa Barata Lopes Jessyane Rodrigues do Nascimento Marcos Bispo Pinheiro Camara Aldilene da Silva Lima Gláucia Laís Nunes Lopes Matheus Oliveira do Nascimento Júlia Karla Albuquerque Melo Xavier Caroline Martins de Jesus Cáritas de Jesus Silva Mendonça André Luis Menezes Carvalho Lucilene Amorim Silva Cláudia Quintino da Rocha Chemical Characterization, Leishmanicidal Activity and In Vitro Cytotoxicity of the Essential Oil Extracted from <i>Pectis brevipedunculata</i> (Gardner) Sch.Bip. and Its Incorporation into Microemulsion Systems Pharmaceutics Asteraceae formulation <i>L.</i> (L.) <i>amazonensis</i> monoterpenes nanotechnology promastigote |
| title | Chemical Characterization, Leishmanicidal Activity and In Vitro Cytotoxicity of the Essential Oil Extracted from <i>Pectis brevipedunculata</i> (Gardner) Sch.Bip. and Its Incorporation into Microemulsion Systems |
| title_full | Chemical Characterization, Leishmanicidal Activity and In Vitro Cytotoxicity of the Essential Oil Extracted from <i>Pectis brevipedunculata</i> (Gardner) Sch.Bip. and Its Incorporation into Microemulsion Systems |
| title_fullStr | Chemical Characterization, Leishmanicidal Activity and In Vitro Cytotoxicity of the Essential Oil Extracted from <i>Pectis brevipedunculata</i> (Gardner) Sch.Bip. and Its Incorporation into Microemulsion Systems |
| title_full_unstemmed | Chemical Characterization, Leishmanicidal Activity and In Vitro Cytotoxicity of the Essential Oil Extracted from <i>Pectis brevipedunculata</i> (Gardner) Sch.Bip. and Its Incorporation into Microemulsion Systems |
| title_short | Chemical Characterization, Leishmanicidal Activity and In Vitro Cytotoxicity of the Essential Oil Extracted from <i>Pectis brevipedunculata</i> (Gardner) Sch.Bip. and Its Incorporation into Microemulsion Systems |
| title_sort | chemical characterization leishmanicidal activity and in vitro cytotoxicity of the essential oil extracted from i pectis brevipedunculata i gardner sch bip and its incorporation into microemulsion systems |
| topic | Asteraceae formulation <i>L.</i> (L.) <i>amazonensis</i> monoterpenes nanotechnology promastigote |
| url | https://www.mdpi.com/1999-4923/16/1/87 |
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