Nucleotide analysis and prevalence of Escherichia coli isolated from feces of some captive avian species
The aim of the study was to check the prevalence of Escherichia coli in some captive avian species, seasonal effect on the E.coli prevalence and analysis of nucleotide sequences of E.coli. A total of 132 samples, 33 from Turkey (Meleagris gallopavo), 33 form Pheasant (Phasianus colchicus), 33 from B...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-01-01
|
| Series: | Journal of King Saud University: Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1018364722005560 |
| _version_ | 1811198039466967040 |
|---|---|
| author | Nimra khalid Syed Mohsin Bukhari Mohammad Y. Alshahrani Khalil Ur Rehman Shahbaz Ahmad Shahla Andleeb Arshad Javid Sheikh Muhammad Azam |
| author_facet | Nimra khalid Syed Mohsin Bukhari Mohammad Y. Alshahrani Khalil Ur Rehman Shahbaz Ahmad Shahla Andleeb Arshad Javid Sheikh Muhammad Azam |
| author_sort | Nimra khalid |
| collection | DOAJ |
| description | The aim of the study was to check the prevalence of Escherichia coli in some captive avian species, seasonal effect on the E.coli prevalence and analysis of nucleotide sequences of E.coli. A total of 132 samples, 33 from Turkey (Meleagris gallopavo), 33 form Pheasant (Phasianus colchicus), 33 from Budgerigar (Melopsittacus undulates) and 33 from Chukar partridge (Alectoris chukar) were collected from Conservation and Research Center, UVAS, Ravi Campus, Pattoki. Colony forming units was quantified for each sample. E. coli confirmation was done by biochemical and molecular characterization. 16S rRNA was amplified and sequenced. 16S rRNA sequence was submitted to NCBI under the accession number MN841017, MN841018 and MN841019.Descriptive statistics showed the mean ± SEM value for E. coli CFU/ml of fecal sample from Turkey 1.91 × 108 ± 4.4 × 107, for Pheasants, the mean ± SEM was 1.55 × 108 ± 5.2 × 107 CFU/ml of fecal sample. The mean ± SEM of the fecal sample for Budgerigars and Chukar were 2.12 × 108 ± 3.3 × 107 CFU/ml and 1.6 × 108 ± 4.5 × 107 CFU/ml respectively. Inferential statistics showed that regardless of the bird species, there was almost a similar frequency of E. coli CFU/ml of fecal sample (p = 0.74). However, the incidence of E. coli fluctuates significantly depending on the season in the case of turkey and pheasants, and the impact was statistically significant (p < 0.0005). E.coli was most prevalent in Turkey during rainy summer and in Pheasants during cool dry winter. These findings show that accidental or direct contact with feces of these captive birds have possible risk of gastric illness to humans and animals. Furthermore, understanding the mechanisms driving the seasonality of this important zoonotic pathogen will allow for the execution of effective control strategies when it is most prevalent. |
| first_indexed | 2024-04-12T01:24:15Z |
| format | Article |
| id | doaj.art-854547e425e246ab9e19fbbc6ae28a9a |
| institution | Directory Open Access Journal |
| issn | 1018-3647 |
| language | English |
| last_indexed | 2024-04-12T01:24:15Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of King Saud University: Science |
| spelling | doaj.art-854547e425e246ab9e19fbbc6ae28a9a2022-12-22T03:53:42ZengElsevierJournal of King Saud University: Science1018-36472023-01-01351102375Nucleotide analysis and prevalence of Escherichia coli isolated from feces of some captive avian speciesNimra khalid0Syed Mohsin Bukhari1Mohammad Y. Alshahrani2Khalil Ur Rehman3Shahbaz Ahmad4Shahla Andleeb5Arshad Javid6Sheikh Muhammad Azam7Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, 8 Lahore 54000, PakistanDepartment of Wildlife and Ecology, University of Veterinary and Animal Sciences, 8 Lahore 54000, Pakistan; Corresponding author.Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, P.O. Box 61413, Abha 9088, Saudi ArabiaDepartment of Environmental Sciences, Faculty of Natural Sciences, GC Women University Sialkot 51310, PakistanDepartment of Entomology, University of Punjab, Lahore, PakistanDepartment of Environmental Sciences, Faculty of Natural Sciences, GC Women University Sialkot 51310, PakistanDepartment of Wildlife and Ecology, University of Veterinary and Animal Sciences, 8 Lahore 54000, PakistanDepartment of Zoology, Division of Science and Technology, University of Education Lahore, PakistanThe aim of the study was to check the prevalence of Escherichia coli in some captive avian species, seasonal effect on the E.coli prevalence and analysis of nucleotide sequences of E.coli. A total of 132 samples, 33 from Turkey (Meleagris gallopavo), 33 form Pheasant (Phasianus colchicus), 33 from Budgerigar (Melopsittacus undulates) and 33 from Chukar partridge (Alectoris chukar) were collected from Conservation and Research Center, UVAS, Ravi Campus, Pattoki. Colony forming units was quantified for each sample. E. coli confirmation was done by biochemical and molecular characterization. 16S rRNA was amplified and sequenced. 16S rRNA sequence was submitted to NCBI under the accession number MN841017, MN841018 and MN841019.Descriptive statistics showed the mean ± SEM value for E. coli CFU/ml of fecal sample from Turkey 1.91 × 108 ± 4.4 × 107, for Pheasants, the mean ± SEM was 1.55 × 108 ± 5.2 × 107 CFU/ml of fecal sample. The mean ± SEM of the fecal sample for Budgerigars and Chukar were 2.12 × 108 ± 3.3 × 107 CFU/ml and 1.6 × 108 ± 4.5 × 107 CFU/ml respectively. Inferential statistics showed that regardless of the bird species, there was almost a similar frequency of E. coli CFU/ml of fecal sample (p = 0.74). However, the incidence of E. coli fluctuates significantly depending on the season in the case of turkey and pheasants, and the impact was statistically significant (p < 0.0005). E.coli was most prevalent in Turkey during rainy summer and in Pheasants during cool dry winter. These findings show that accidental or direct contact with feces of these captive birds have possible risk of gastric illness to humans and animals. Furthermore, understanding the mechanisms driving the seasonality of this important zoonotic pathogen will allow for the execution of effective control strategies when it is most prevalent.http://www.sciencedirect.com/science/article/pii/S1018364722005560Captive avian speciesEscherichia coliPrevalenceFecal sample16S rRNA gene |
| spellingShingle | Nimra khalid Syed Mohsin Bukhari Mohammad Y. Alshahrani Khalil Ur Rehman Shahbaz Ahmad Shahla Andleeb Arshad Javid Sheikh Muhammad Azam Nucleotide analysis and prevalence of Escherichia coli isolated from feces of some captive avian species Journal of King Saud University: Science Captive avian species Escherichia coli Prevalence Fecal sample 16S rRNA gene |
| title | Nucleotide analysis and prevalence of Escherichia coli isolated from feces of some captive avian species |
| title_full | Nucleotide analysis and prevalence of Escherichia coli isolated from feces of some captive avian species |
| title_fullStr | Nucleotide analysis and prevalence of Escherichia coli isolated from feces of some captive avian species |
| title_full_unstemmed | Nucleotide analysis and prevalence of Escherichia coli isolated from feces of some captive avian species |
| title_short | Nucleotide analysis and prevalence of Escherichia coli isolated from feces of some captive avian species |
| title_sort | nucleotide analysis and prevalence of escherichia coli isolated from feces of some captive avian species |
| topic | Captive avian species Escherichia coli Prevalence Fecal sample 16S rRNA gene |
| url | http://www.sciencedirect.com/science/article/pii/S1018364722005560 |
| work_keys_str_mv | AT nimrakhalid nucleotideanalysisandprevalenceofescherichiacoliisolatedfromfecesofsomecaptiveavianspecies AT syedmohsinbukhari nucleotideanalysisandprevalenceofescherichiacoliisolatedfromfecesofsomecaptiveavianspecies AT mohammadyalshahrani nucleotideanalysisandprevalenceofescherichiacoliisolatedfromfecesofsomecaptiveavianspecies AT khalilurrehman nucleotideanalysisandprevalenceofescherichiacoliisolatedfromfecesofsomecaptiveavianspecies AT shahbazahmad nucleotideanalysisandprevalenceofescherichiacoliisolatedfromfecesofsomecaptiveavianspecies AT shahlaandleeb nucleotideanalysisandprevalenceofescherichiacoliisolatedfromfecesofsomecaptiveavianspecies AT arshadjavid nucleotideanalysisandprevalenceofescherichiacoliisolatedfromfecesofsomecaptiveavianspecies AT sheikhmuhammadazam nucleotideanalysisandprevalenceofescherichiacoliisolatedfromfecesofsomecaptiveavianspecies |