Removal of Total Petroleum Hydrocarbon (TPH) crude oil by consortium bacteria acetobacter tropicalis and lactobacillus casei
The objective of this research is to remove Total Petroleum Hydrocarbon (TPH) contained in crude oil by utilizing Acetobacter tropicalis and Lactobacillus casei bacteria consortium through degradation process. Crude oil degradation was conducted on Stone Mineral Salt Solution (SMSS) liquid media and...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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EDP Sciences
2023-01-01
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| Series: | E3S Web of Conferences |
| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/57/e3sconf_ebwff2023_09009.pdf |
| _version_ | 1797673729549926400 |
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| author | Azzahra F. Rinanti A. Hadisoebroto Rositayanti Minarti A. Aphirta S. Rahmiyati L. Mahrendra S.M.P. Sunaryo T. |
| author_facet | Azzahra F. Rinanti A. Hadisoebroto Rositayanti Minarti A. Aphirta S. Rahmiyati L. Mahrendra S.M.P. Sunaryo T. |
| author_sort | Azzahra F. |
| collection | DOAJ |
| description | The objective of this research is to remove Total Petroleum Hydrocarbon (TPH) contained in crude oil by utilizing Acetobacter tropicalis and Lactobacillus casei bacteria consortium through degradation process. Crude oil degradation was conducted on Stone Mineral Salt Solution (SMSS) liquid media and artificial sea water in a controlled environment with limited system. The implemented variations on SMSS liquid media are differentiated based on acidity (pH) of 3, 5, and 7, with contact time of 7, 14, and 21 days. On the other hand, the variation implemented on artificial sea water only applied on contact time of 7, 14, and 21 days. Samples were incubated on a shaker incubator with 30°C and 150 rpm. The sensitivity test revealed that consortiom bacteria are resistant against crude oil, which proven by the missing inhibiting zone formation around disc paper that contains crude oil. This research shows that the optimum condition to degreade TPH both on SMSS liquid media and artificial sea water is at pH level of 7 in 7 days with TPH removal efficiency of 94%. This research provides an important information that Acetobacter tropicalis and Lactobacillus casei bacteria consortium has the potential to degrade crude oil TPH in a controlled environment. |
| first_indexed | 2024-03-11T21:48:38Z |
| format | Article |
| id | doaj.art-854580cd870f4bbd81263efdff56b656 |
| institution | Directory Open Access Journal |
| issn | 2267-1242 |
| language | English |
| last_indexed | 2024-03-11T21:48:38Z |
| publishDate | 2023-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj.art-854580cd870f4bbd81263efdff56b6562023-09-26T10:10:43ZengEDP SciencesE3S Web of Conferences2267-12422023-01-014200900910.1051/e3sconf/202342009009e3sconf_ebwff2023_09009Removal of Total Petroleum Hydrocarbon (TPH) crude oil by consortium bacteria acetobacter tropicalis and lactobacillus caseiAzzahra F.0Rinanti A.1Hadisoebroto Rositayanti2Minarti A.3Aphirta S.4Rahmiyati L.5Mahrendra S.M.P.6Sunaryo T.7Faculty of Landscape Architecture and Environmental Technology, Department of Environmental Engineering Universitas TrisaktiFaculty of Landscape Architecture and Environmental Technology, Department of Environmental Engineering Universitas TrisaktiFaculty of Landscape Architecture and Environmental Technology, Department of Environmental Engineering Universitas TrisaktiFaculty of Landscape Architecture and Environmental Technology, Department of Environmental Engineering Universitas TrisaktiFaculty of Landscape Architecture and Environmental Technology, Department of Environmental Engineering Universitas TrisaktiFaculty of Landscape Architecture and Environmental Technology, Department of Environmental Engineering Universitas TrisaktiFaculty of Landscape Architecture and Environmental Technology, Department of Environmental Engineering Universitas TrisaktiFaculty of Landscape Architecture and Environmental Technology, Department of Environmental Engineering Universitas TrisaktiThe objective of this research is to remove Total Petroleum Hydrocarbon (TPH) contained in crude oil by utilizing Acetobacter tropicalis and Lactobacillus casei bacteria consortium through degradation process. Crude oil degradation was conducted on Stone Mineral Salt Solution (SMSS) liquid media and artificial sea water in a controlled environment with limited system. The implemented variations on SMSS liquid media are differentiated based on acidity (pH) of 3, 5, and 7, with contact time of 7, 14, and 21 days. On the other hand, the variation implemented on artificial sea water only applied on contact time of 7, 14, and 21 days. Samples were incubated on a shaker incubator with 30°C and 150 rpm. The sensitivity test revealed that consortiom bacteria are resistant against crude oil, which proven by the missing inhibiting zone formation around disc paper that contains crude oil. This research shows that the optimum condition to degreade TPH both on SMSS liquid media and artificial sea water is at pH level of 7 in 7 days with TPH removal efficiency of 94%. This research provides an important information that Acetobacter tropicalis and Lactobacillus casei bacteria consortium has the potential to degrade crude oil TPH in a controlled environment.https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/57/e3sconf_ebwff2023_09009.pdf |
| spellingShingle | Azzahra F. Rinanti A. Hadisoebroto Rositayanti Minarti A. Aphirta S. Rahmiyati L. Mahrendra S.M.P. Sunaryo T. Removal of Total Petroleum Hydrocarbon (TPH) crude oil by consortium bacteria acetobacter tropicalis and lactobacillus casei E3S Web of Conferences |
| title | Removal of Total Petroleum Hydrocarbon (TPH) crude oil by consortium bacteria acetobacter tropicalis and lactobacillus casei |
| title_full | Removal of Total Petroleum Hydrocarbon (TPH) crude oil by consortium bacteria acetobacter tropicalis and lactobacillus casei |
| title_fullStr | Removal of Total Petroleum Hydrocarbon (TPH) crude oil by consortium bacteria acetobacter tropicalis and lactobacillus casei |
| title_full_unstemmed | Removal of Total Petroleum Hydrocarbon (TPH) crude oil by consortium bacteria acetobacter tropicalis and lactobacillus casei |
| title_short | Removal of Total Petroleum Hydrocarbon (TPH) crude oil by consortium bacteria acetobacter tropicalis and lactobacillus casei |
| title_sort | removal of total petroleum hydrocarbon tph crude oil by consortium bacteria acetobacter tropicalis and lactobacillus casei |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/57/e3sconf_ebwff2023_09009.pdf |
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