Functional nucleic acid-based biosensors for virus detection
A growing number of viruses pose significant threats to human health, impact agricultural production and compromise food safety. Minimizing the impact of viral infections demands for effective technologies that can deliver sensitive viral detection under various settings. Functional nucleic acids (F...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2023-09-01
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| Series: | Advanced Agrochem |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2773237123000539 |
| _version_ | 1797235472709189632 |
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| author | Zijie Zhang Bal Ram Adhikari Payel Sen Leyla Soleymani Yingfu Li |
| author_facet | Zijie Zhang Bal Ram Adhikari Payel Sen Leyla Soleymani Yingfu Li |
| author_sort | Zijie Zhang |
| collection | DOAJ |
| description | A growing number of viruses pose significant threats to human health, impact agricultural production and compromise food safety. Minimizing the impact of viral infections demands for effective technologies that can deliver sensitive viral detection under various settings. Functional nucleic acids (FNAs) – natural or synthetic nucleic acid molecules with catalytic and/or binding properties – have been increasingly explored as key components for setting up diagnostic tests for viral detection. FNA-based viral biosensors offer distinct advantages, such as the ability to achieve sensitive viral detection in complex biological samples, ease of use, scalability, and cost-effectiveness. This mini-review will be dedicated to appraising progress made in recent years in FNA-based biosensors for diagnosing diverse human and animal viruses. We will first describe virus structures and potential biomarkers that can be utilized as the targets for FNA probe engineering. This is followed by reviewing the latest advances in two classes of FNAs - DNAzyme and DNA aptamers, with focuses on DNAzyme- and DNA aptamer-based biosensors that employ diverse signal transduction mechanisms to achieve sensitive viral detections. Finally, we will discuss current challenges in the field and suggest future research directions that can address these challenges. |
| first_indexed | 2024-04-24T16:48:30Z |
| format | Article |
| id | doaj.art-85475506ce3b43edb1ee8b27a2a88bf8 |
| institution | Directory Open Access Journal |
| issn | 2773-2371 |
| language | English |
| last_indexed | 2024-04-24T16:48:30Z |
| publishDate | 2023-09-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Advanced Agrochem |
| spelling | doaj.art-85475506ce3b43edb1ee8b27a2a88bf82024-03-29T05:51:42ZengKeAi Communications Co., Ltd.Advanced Agrochem2773-23712023-09-0123246257Functional nucleic acid-based biosensors for virus detectionZijie Zhang0Bal Ram Adhikari1Payel Sen2Leyla Soleymani3Yingfu Li4Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4K1, CanadaDepartment of Engineering Physics, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4K1, CanadaDepartment of Engineering Physics, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4K1, CanadaDepartment of Engineering Physics, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4K1, Canada; School of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4K1, Canada; Corresponding author. Department of Engineering Physics, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4K1, Canada.Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4K1, Canada; School of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4K1, Canada; Corresponding author. Department of Biochemistry and Biomedical Sciences, McMaster University, 1280 Main Street West, Hamilton, Ontario, L8S 4K1, Canada.A growing number of viruses pose significant threats to human health, impact agricultural production and compromise food safety. Minimizing the impact of viral infections demands for effective technologies that can deliver sensitive viral detection under various settings. Functional nucleic acids (FNAs) – natural or synthetic nucleic acid molecules with catalytic and/or binding properties – have been increasingly explored as key components for setting up diagnostic tests for viral detection. FNA-based viral biosensors offer distinct advantages, such as the ability to achieve sensitive viral detection in complex biological samples, ease of use, scalability, and cost-effectiveness. This mini-review will be dedicated to appraising progress made in recent years in FNA-based biosensors for diagnosing diverse human and animal viruses. We will first describe virus structures and potential biomarkers that can be utilized as the targets for FNA probe engineering. This is followed by reviewing the latest advances in two classes of FNAs - DNAzyme and DNA aptamers, with focuses on DNAzyme- and DNA aptamer-based biosensors that employ diverse signal transduction mechanisms to achieve sensitive viral detections. Finally, we will discuss current challenges in the field and suggest future research directions that can address these challenges.http://www.sciencedirect.com/science/article/pii/S2773237123000539Functional nucleic acidsBiosensorsAptamersDNAzymesVirusesInfectious diseases |
| spellingShingle | Zijie Zhang Bal Ram Adhikari Payel Sen Leyla Soleymani Yingfu Li Functional nucleic acid-based biosensors for virus detection Advanced Agrochem Functional nucleic acids Biosensors Aptamers DNAzymes Viruses Infectious diseases |
| title | Functional nucleic acid-based biosensors for virus detection |
| title_full | Functional nucleic acid-based biosensors for virus detection |
| title_fullStr | Functional nucleic acid-based biosensors for virus detection |
| title_full_unstemmed | Functional nucleic acid-based biosensors for virus detection |
| title_short | Functional nucleic acid-based biosensors for virus detection |
| title_sort | functional nucleic acid based biosensors for virus detection |
| topic | Functional nucleic acids Biosensors Aptamers DNAzymes Viruses Infectious diseases |
| url | http://www.sciencedirect.com/science/article/pii/S2773237123000539 |
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