EFFECT OF NEEM AND WILLOW AQUEOUS EXTRACTS ON FUSARIUM WILT DISEASE IN TOMATO SEEDLINGS: 1-INDUCTION OF ANTIOXIDANT DEFENSIVE ENZYMES
Fusarium wilt disease is one of the major plant diseases that affect tomato production. The effects of neem (Azadirachta indica) and willow (Salix babylonica) aqueous extracts on fusarium wilt disease in tomato seedlings were investigated. Four weeks old tomato seedlings were treated with 10% of eit...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | Arabic |
| Published: |
The Union of Arab Universities
2011-03-01
|
| Series: | Arab Universities Journal of Agricultural Sciences |
| Subjects: | |
| Online Access: | https://ajs.journals.ekb.eg/article_14624_f733bc7300d20f562fa7f36bf2c8416a.pdf |
| _version_ | 1797269673524330496 |
|---|---|
| author | , R.M Farag Hanaa Abdou Zeinab A. Salama Dawlat A. Ibrahim Mervat A.R. Sror H.A.M. |
| author_facet | , R.M Farag Hanaa Abdou Zeinab A. Salama Dawlat A. Ibrahim Mervat A.R. Sror H.A.M. |
| author_sort | , R.M Farag Hanaa |
| collection | DOAJ |
| description | Fusarium wilt disease is one of the major plant diseases that affect tomato production. The effects of neem (Azadirachta indica) and willow (Salix babylonica) aqueous extracts on fusarium wilt disease in tomato seedlings were investigated. Four weeks old tomato seedlings were treated with 10% of either neem and willow aqueous extracts and then infected with Fusarium oxysporum after 4 days of treatment. The results showed that the percentage of disease incidence was increased in non treated tomato seedlings in time dependent manner and reached the maximum level (65%) after 6 weeks of infection. Treatments of tomato plants with neem and willow aqueous extracts reduced the percentage of disease incidence to the level of 25.5% and 27.8% after 6 weeks of infection respectively. The results show that infection of tomato seedling with Fusarium oxysporum led to many morphological and biochemical changes including, reducing the growth of tomato shoot and root, increasing the level of lipid peroxidation and marked increase in the activities of antioxidant defensive enzyme i.e. POX, CAT, and SOD. Treatment with neem and willow aqueous extracts significantly exhibited a growth promotion of tomato shoot and root in infected or non infected seedling. Moreover, application of neem and willow aqueous extracts with fusarium, significantly reduce the level of lipid peroxidation and induce high activities of antioxidant defensive enzymes after 3 and 7 days of infection. Electrophoretic pattern of POX demonstrated that Fusarium oxysporum caused up regulation of several POX isoenzymes. It could be concluded that neem and willow aqueous extracts reduced the disease incidence of fusarium wilt in tomato seedlings by increasing the activities of antioxidant defensive enzymes and decreasing the level of lipid peroxidation. |
| first_indexed | 2024-04-25T01:52:07Z |
| format | Article |
| id | doaj.art-854a4dd7700c430f97d9247bce4b81cb |
| institution | Directory Open Access Journal |
| issn | 1110-2675 2636-3585 |
| language | Arabic |
| last_indexed | 2024-04-25T01:52:07Z |
| publishDate | 2011-03-01 |
| publisher | The Union of Arab Universities |
| record_format | Article |
| series | Arab Universities Journal of Agricultural Sciences |
| spelling | doaj.art-854a4dd7700c430f97d9247bce4b81cb2024-03-07T17:45:10ZaraThe Union of Arab UniversitiesArab Universities Journal of Agricultural Sciences1110-26752636-35852011-03-0119113114010.21608/ajs.2011.1462414624EFFECT OF NEEM AND WILLOW AQUEOUS EXTRACTS ON FUSARIUM WILT DISEASE IN TOMATO SEEDLINGS: 1-INDUCTION OF ANTIOXIDANT DEFENSIVE ENZYMES, R.M Farag Hanaa0Abdou Zeinab A.1Salama Dawlat A.2Ibrahim Mervat A.R.3Sror H.A.M.4Agricultural Biochemistry Dept., Fac. Agric. Ain Shams Univ., Shoubra Elkhema, Cairo, EgyptAgricultural Biochemistry Dept., Fac. Agric. Ain Shams Univ., Shoubra Elkhema, Cairo, EgyptAgricultural Biochemistry Dept., Fac. Agric. Ain Shams Univ., Shoubra Elkhema, Cairo, EgyptAgricultural Biochemistry Dept., Fac. Agric. Ain Shams Univ., Shoubra Elkhema, Cairo, EgyptAgricultural Biochemistry Dept., Fac. Agric. Ain Shams Univ., Shoubra Elkhema, Cairo, EgyptFusarium wilt disease is one of the major plant diseases that affect tomato production. The effects of neem (Azadirachta indica) and willow (Salix babylonica) aqueous extracts on fusarium wilt disease in tomato seedlings were investigated. Four weeks old tomato seedlings were treated with 10% of either neem and willow aqueous extracts and then infected with Fusarium oxysporum after 4 days of treatment. The results showed that the percentage of disease incidence was increased in non treated tomato seedlings in time dependent manner and reached the maximum level (65%) after 6 weeks of infection. Treatments of tomato plants with neem and willow aqueous extracts reduced the percentage of disease incidence to the level of 25.5% and 27.8% after 6 weeks of infection respectively. The results show that infection of tomato seedling with Fusarium oxysporum led to many morphological and biochemical changes including, reducing the growth of tomato shoot and root, increasing the level of lipid peroxidation and marked increase in the activities of antioxidant defensive enzyme i.e. POX, CAT, and SOD. Treatment with neem and willow aqueous extracts significantly exhibited a growth promotion of tomato shoot and root in infected or non infected seedling. Moreover, application of neem and willow aqueous extracts with fusarium, significantly reduce the level of lipid peroxidation and induce high activities of antioxidant defensive enzymes after 3 and 7 days of infection. Electrophoretic pattern of POX demonstrated that Fusarium oxysporum caused up regulation of several POX isoenzymes. It could be concluded that neem and willow aqueous extracts reduced the disease incidence of fusarium wilt in tomato seedlings by increasing the activities of antioxidant defensive enzymes and decreasing the level of lipid peroxidation.https://ajs.journals.ekb.eg/article_14624_f733bc7300d20f562fa7f36bf2c8416a.pdfwillowneemtomato seedlingsfusarium oxysporumantioxidant enzymes |
| spellingShingle | , R.M Farag Hanaa Abdou Zeinab A. Salama Dawlat A. Ibrahim Mervat A.R. Sror H.A.M. EFFECT OF NEEM AND WILLOW AQUEOUS EXTRACTS ON FUSARIUM WILT DISEASE IN TOMATO SEEDLINGS: 1-INDUCTION OF ANTIOXIDANT DEFENSIVE ENZYMES Arab Universities Journal of Agricultural Sciences willow neem tomato seedlings fusarium oxysporum antioxidant enzymes |
| title | EFFECT OF NEEM AND WILLOW AQUEOUS EXTRACTS ON FUSARIUM WILT DISEASE IN TOMATO SEEDLINGS: 1-INDUCTION OF ANTIOXIDANT DEFENSIVE ENZYMES |
| title_full | EFFECT OF NEEM AND WILLOW AQUEOUS EXTRACTS ON FUSARIUM WILT DISEASE IN TOMATO SEEDLINGS: 1-INDUCTION OF ANTIOXIDANT DEFENSIVE ENZYMES |
| title_fullStr | EFFECT OF NEEM AND WILLOW AQUEOUS EXTRACTS ON FUSARIUM WILT DISEASE IN TOMATO SEEDLINGS: 1-INDUCTION OF ANTIOXIDANT DEFENSIVE ENZYMES |
| title_full_unstemmed | EFFECT OF NEEM AND WILLOW AQUEOUS EXTRACTS ON FUSARIUM WILT DISEASE IN TOMATO SEEDLINGS: 1-INDUCTION OF ANTIOXIDANT DEFENSIVE ENZYMES |
| title_short | EFFECT OF NEEM AND WILLOW AQUEOUS EXTRACTS ON FUSARIUM WILT DISEASE IN TOMATO SEEDLINGS: 1-INDUCTION OF ANTIOXIDANT DEFENSIVE ENZYMES |
| title_sort | effect of neem and willow aqueous extracts on fusarium wilt disease in tomato seedlings 1 induction of antioxidant defensive enzymes |
| topic | willow neem tomato seedlings fusarium oxysporum antioxidant enzymes |
| url | https://ajs.journals.ekb.eg/article_14624_f733bc7300d20f562fa7f36bf2c8416a.pdf |
| work_keys_str_mv | AT rmfaraghanaa effectofneemandwillowaqueousextractsonfusariumwiltdiseaseintomatoseedlings1inductionofantioxidantdefensiveenzymes AT abdouzeinaba effectofneemandwillowaqueousextractsonfusariumwiltdiseaseintomatoseedlings1inductionofantioxidantdefensiveenzymes AT salamadawlata effectofneemandwillowaqueousextractsonfusariumwiltdiseaseintomatoseedlings1inductionofantioxidantdefensiveenzymes AT ibrahimmervatar effectofneemandwillowaqueousextractsonfusariumwiltdiseaseintomatoseedlings1inductionofantioxidantdefensiveenzymes AT srorham effectofneemandwillowaqueousextractsonfusariumwiltdiseaseintomatoseedlings1inductionofantioxidantdefensiveenzymes |