Persistent Ventricle Partitioning in the Adult Zebrafish Heart
The vertebrate heart integrates cells from the early-differentiating first heart field (FHF) and the later-differentiating second heart field (SHF), both emerging from the lateral plate mesoderm. In mammals, this process forms the basis for the development of the left and right ventricle chambers an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Journal of Cardiovascular Development and Disease |
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| Online Access: | https://www.mdpi.com/2308-3425/8/4/41 |
| _version_ | 1797538266633732096 |
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| author | Catherine Pfefferli Hannah R. Moran Anastasia Felker Christian Mosimann Anna Jaźwińska |
| author_facet | Catherine Pfefferli Hannah R. Moran Anastasia Felker Christian Mosimann Anna Jaźwińska |
| author_sort | Catherine Pfefferli |
| collection | DOAJ |
| description | The vertebrate heart integrates cells from the early-differentiating first heart field (FHF) and the later-differentiating second heart field (SHF), both emerging from the lateral plate mesoderm. In mammals, this process forms the basis for the development of the left and right ventricle chambers and subsequent chamber septation. The single ventricle-forming zebrafish heart also integrates FHF and SHF lineages during embryogenesis, yet the contributions of these two myocardial lineages to the adult zebrafish heart remain incompletely understood. Here, we characterize the myocardial labeling of FHF descendants in both the developing and adult zebrafish ventricle. Expanding previous findings, late gastrulation-stage labeling using <i>drl</i>-driven CreERT2 recombinase with a myocardium-specific, <i>myl7</i>-controlled, <i>loxP</i> reporter results in the predominant labeling of FHF-derived outer curvature and the right side of the embryonic ventricle. Raised to adulthood, such lineage-labeled hearts retain broad areas of FHF cardiomyocytes in a region of the ventricle that is positioned at the opposite side to the atrium and encompasses the apex. Our data add to the increasing evidence for a persisting cell-based compartmentalization of the adult zebrafish ventricle even in the absence of any physical boundary. |
| first_indexed | 2024-03-10T12:28:05Z |
| format | Article |
| id | doaj.art-854db09f9ab74142a453ac9e116c7be5 |
| institution | Directory Open Access Journal |
| issn | 2308-3425 |
| language | English |
| last_indexed | 2024-03-10T12:28:05Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Cardiovascular Development and Disease |
| spelling | doaj.art-854db09f9ab74142a453ac9e116c7be52023-11-21T14:51:54ZengMDPI AGJournal of Cardiovascular Development and Disease2308-34252021-04-01844110.3390/jcdd8040041Persistent Ventricle Partitioning in the Adult Zebrafish HeartCatherine Pfefferli0Hannah R. Moran1Anastasia Felker2Christian Mosimann3Anna Jaźwińska4Department of Biology, University of Fribourg, Chemin du Musée 10, 1700 Fribourg, SwitzerlandDepartment of Pediatrics, Section of Developmental Biology, University of Colorado School of Medicine and Children’s Hospital Colorado, Anschutz Medical Campus, Aurora, CO 80045, USADepartment of Pediatrics, Section of Developmental Biology, University of Colorado School of Medicine and Children’s Hospital Colorado, Anschutz Medical Campus, Aurora, CO 80045, USADepartment of Pediatrics, Section of Developmental Biology, University of Colorado School of Medicine and Children’s Hospital Colorado, Anschutz Medical Campus, Aurora, CO 80045, USADepartment of Biology, University of Fribourg, Chemin du Musée 10, 1700 Fribourg, SwitzerlandThe vertebrate heart integrates cells from the early-differentiating first heart field (FHF) and the later-differentiating second heart field (SHF), both emerging from the lateral plate mesoderm. In mammals, this process forms the basis for the development of the left and right ventricle chambers and subsequent chamber septation. The single ventricle-forming zebrafish heart also integrates FHF and SHF lineages during embryogenesis, yet the contributions of these two myocardial lineages to the adult zebrafish heart remain incompletely understood. Here, we characterize the myocardial labeling of FHF descendants in both the developing and adult zebrafish ventricle. Expanding previous findings, late gastrulation-stage labeling using <i>drl</i>-driven CreERT2 recombinase with a myocardium-specific, <i>myl7</i>-controlled, <i>loxP</i> reporter results in the predominant labeling of FHF-derived outer curvature and the right side of the embryonic ventricle. Raised to adulthood, such lineage-labeled hearts retain broad areas of FHF cardiomyocytes in a region of the ventricle that is positioned at the opposite side to the atrium and encompasses the apex. Our data add to the increasing evidence for a persisting cell-based compartmentalization of the adult zebrafish ventricle even in the absence of any physical boundary.https://www.mdpi.com/2308-3425/8/4/41heart developmentfirst heart fieldlineage tracingcardiac ventriclezebrafish |
| spellingShingle | Catherine Pfefferli Hannah R. Moran Anastasia Felker Christian Mosimann Anna Jaźwińska Persistent Ventricle Partitioning in the Adult Zebrafish Heart Journal of Cardiovascular Development and Disease heart development first heart field lineage tracing cardiac ventricle zebrafish |
| title | Persistent Ventricle Partitioning in the Adult Zebrafish Heart |
| title_full | Persistent Ventricle Partitioning in the Adult Zebrafish Heart |
| title_fullStr | Persistent Ventricle Partitioning in the Adult Zebrafish Heart |
| title_full_unstemmed | Persistent Ventricle Partitioning in the Adult Zebrafish Heart |
| title_short | Persistent Ventricle Partitioning in the Adult Zebrafish Heart |
| title_sort | persistent ventricle partitioning in the adult zebrafish heart |
| topic | heart development first heart field lineage tracing cardiac ventricle zebrafish |
| url | https://www.mdpi.com/2308-3425/8/4/41 |
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