The effects of 6-gingerol on proliferation, differentiation, and maturation of osteoblast-like MG-63 cells
We investigated whether 6-gingerol affects the maturation and proliferation of osteoblast-like MG63 cells in vitro. Osteoblast-like MG63 cells were treated with 6-gingerol under control conditions, and experimental inflammation was induced by tumor necrosis factor-α (TNF-α). Expression of different...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2015-07-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700637&lng=en&tlng=en |
| _version_ | 1819179673159991296 |
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| author | J.Z. Fan X. Yang Z.G. Bi |
| author_facet | J.Z. Fan X. Yang Z.G. Bi |
| author_sort | J.Z. Fan |
| collection | DOAJ |
| description | We investigated whether 6-gingerol affects the maturation and proliferation of osteoblast-like MG63 cells in vitro. Osteoblast-like MG63 cells were treated with 6-gingerol under control conditions, and experimental inflammation was induced by tumor necrosis factor-α (TNF-α). Expression of different osteogenic markers and cytokines was analyzed by real-time PCR, Western blotting, and enzyme-linked immunosorbent assay. In addition, alkaline phosphatase (ALP) enzyme activity and biomineralization as markers for differentiation were measured. Treatment with 6-gingerol resulted in insignificant effects on the proliferation rate. 6-Gingerol induced the differentiation of osteoblast-like cells with increased transcription levels of osteogenic markers, upregulated ALP enzyme activity, and enhanced mineralized nodule formation. Stimulation with TNF-α led to enhanced interleukin-6 and nuclear factor-κB expression and downregulated markers of osteoblastic differentiation. 6-Gingerol reduced the degree of inflammation in TNF-α-treated MG-63 cells. In conclusion, 6-gingerol stimulated osteoblast differentiation in normal physiological and inflammatory settings, and therefore, 6-gingerol represents a promising agent for treating osteoporosis or bone inflammation. |
| first_indexed | 2024-12-22T22:02:11Z |
| format | Article |
| id | doaj.art-854f89852bce4c70bb452739d6d7c2b4 |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-12-22T22:02:11Z |
| publishDate | 2015-07-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-854f89852bce4c70bb452739d6d7c2b42022-12-21T18:11:05ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2015-07-0148763764310.1590/1414-431X20154494S0100-879X2015000700637The effects of 6-gingerol on proliferation, differentiation, and maturation of osteoblast-like MG-63 cellsJ.Z. FanX. YangZ.G. BiWe investigated whether 6-gingerol affects the maturation and proliferation of osteoblast-like MG63 cells in vitro. Osteoblast-like MG63 cells were treated with 6-gingerol under control conditions, and experimental inflammation was induced by tumor necrosis factor-α (TNF-α). Expression of different osteogenic markers and cytokines was analyzed by real-time PCR, Western blotting, and enzyme-linked immunosorbent assay. In addition, alkaline phosphatase (ALP) enzyme activity and biomineralization as markers for differentiation were measured. Treatment with 6-gingerol resulted in insignificant effects on the proliferation rate. 6-Gingerol induced the differentiation of osteoblast-like cells with increased transcription levels of osteogenic markers, upregulated ALP enzyme activity, and enhanced mineralized nodule formation. Stimulation with TNF-α led to enhanced interleukin-6 and nuclear factor-κB expression and downregulated markers of osteoblastic differentiation. 6-Gingerol reduced the degree of inflammation in TNF-α-treated MG-63 cells. In conclusion, 6-gingerol stimulated osteoblast differentiation in normal physiological and inflammatory settings, and therefore, 6-gingerol represents a promising agent for treating osteoporosis or bone inflammation.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700637&lng=en&tlng=en6-GingerolInterleukin-6Nuclear factor-κBOsteogenesisOsteoblast |
| spellingShingle | J.Z. Fan X. Yang Z.G. Bi The effects of 6-gingerol on proliferation, differentiation, and maturation of osteoblast-like MG-63 cells Brazilian Journal of Medical and Biological Research 6-Gingerol Interleukin-6 Nuclear factor-κB Osteogenesis Osteoblast |
| title | The effects of 6-gingerol on proliferation, differentiation, and maturation of osteoblast-like MG-63 cells |
| title_full | The effects of 6-gingerol on proliferation, differentiation, and maturation of osteoblast-like MG-63 cells |
| title_fullStr | The effects of 6-gingerol on proliferation, differentiation, and maturation of osteoblast-like MG-63 cells |
| title_full_unstemmed | The effects of 6-gingerol on proliferation, differentiation, and maturation of osteoblast-like MG-63 cells |
| title_short | The effects of 6-gingerol on proliferation, differentiation, and maturation of osteoblast-like MG-63 cells |
| title_sort | effects of 6 gingerol on proliferation differentiation and maturation of osteoblast like mg 63 cells |
| topic | 6-Gingerol Interleukin-6 Nuclear factor-κB Osteogenesis Osteoblast |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000700637&lng=en&tlng=en |
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