Determination of the differentiation capacities of murines' primary mononucleated cells and MC3T3-E1 cells
<p>Abstract</p> <p>Background</p> <p>The main morphological features of primitive cells, such as stem and progenitor cells, are that these cells consists only one nucleus. The main purpose of this study was to determine the differentiation capacities of stem and progeni...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-10-01
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| Series: | Cancer Cell International |
| Online Access: | http://www.cancerci.com/content/10/1/42 |
| _version_ | 1818917802336059392 |
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| author | Razak Mohamad Senafi Sahidan Ariffin Shahrul Yazid Muhammad Wahab Rohaya |
| author_facet | Razak Mohamad Senafi Sahidan Ariffin Shahrul Yazid Muhammad Wahab Rohaya |
| author_sort | Razak Mohamad |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The main morphological features of primitive cells, such as stem and progenitor cells, are that these cells consists only one nucleus. The main purpose of this study was to determine the differentiation capacities of stem and progenitor cells. This study was performed using mononucleated cells originated from murine peripheral blood and MC3T3-E1 cells. Three approaches were used to determine their differentiation capacities: 1) Biochemical assays, 2) Gene expression analysis, and 3) Morphological observations.</p> <p>Results</p> <p>We found that both cells were able to differentiate into mature osteoblasts, as assayed by ALP activity. RT-PCR analysis showed the activation of the <it>Opn </it>gene after osteoblast differentiation. Morphological observations of both cells revealed the formation of black or dark-brown nodules after von Kossa staining. Nevertheless, only mononucleated cells showed the significant increase in TRAP activity characteristic of mature osteoclasts. The osteoclast-specific <it>CatK </it>gene was only upregulated in mononucleated cells. Morphological observations indicated the existence of multinucleated osteoclasts. <it>Sca-1 </it>was activated only in undifferentiated mononucleated cells, indicating that the cells were hematopoietic stem cells. In both cell lines, the housekeeping <it>Gapdh </it>gene was activated before and after differentiation.</p> <p>Conclusion</p> <p>The isolated mononucleated cells were able to differentiate into both osteoblasts and osteoclasts; indicating that they are stem cells. On the other hand, MC3T3-E1 cells can only differentiate into osteoblasts; a characteristic of progenitor cells.</p> |
| first_indexed | 2024-12-20T00:39:51Z |
| format | Article |
| id | doaj.art-85505eca3a834dc194e03c0e91fceec3 |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-12-20T00:39:51Z |
| publishDate | 2010-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-85505eca3a834dc194e03c0e91fceec32022-12-21T19:59:38ZengBMCCancer Cell International1475-28672010-10-011014210.1186/1475-2867-10-42Determination of the differentiation capacities of murines' primary mononucleated cells and MC3T3-E1 cellsRazak MohamadSenafi SahidanAriffin ShahrulYazid MuhammadWahab Rohaya<p>Abstract</p> <p>Background</p> <p>The main morphological features of primitive cells, such as stem and progenitor cells, are that these cells consists only one nucleus. The main purpose of this study was to determine the differentiation capacities of stem and progenitor cells. This study was performed using mononucleated cells originated from murine peripheral blood and MC3T3-E1 cells. Three approaches were used to determine their differentiation capacities: 1) Biochemical assays, 2) Gene expression analysis, and 3) Morphological observations.</p> <p>Results</p> <p>We found that both cells were able to differentiate into mature osteoblasts, as assayed by ALP activity. RT-PCR analysis showed the activation of the <it>Opn </it>gene after osteoblast differentiation. Morphological observations of both cells revealed the formation of black or dark-brown nodules after von Kossa staining. Nevertheless, only mononucleated cells showed the significant increase in TRAP activity characteristic of mature osteoclasts. The osteoclast-specific <it>CatK </it>gene was only upregulated in mononucleated cells. Morphological observations indicated the existence of multinucleated osteoclasts. <it>Sca-1 </it>was activated only in undifferentiated mononucleated cells, indicating that the cells were hematopoietic stem cells. In both cell lines, the housekeeping <it>Gapdh </it>gene was activated before and after differentiation.</p> <p>Conclusion</p> <p>The isolated mononucleated cells were able to differentiate into both osteoblasts and osteoclasts; indicating that they are stem cells. On the other hand, MC3T3-E1 cells can only differentiate into osteoblasts; a characteristic of progenitor cells.</p>http://www.cancerci.com/content/10/1/42 |
| spellingShingle | Razak Mohamad Senafi Sahidan Ariffin Shahrul Yazid Muhammad Wahab Rohaya Determination of the differentiation capacities of murines' primary mononucleated cells and MC3T3-E1 cells Cancer Cell International |
| title | Determination of the differentiation capacities of murines' primary mononucleated cells and MC3T3-E1 cells |
| title_full | Determination of the differentiation capacities of murines' primary mononucleated cells and MC3T3-E1 cells |
| title_fullStr | Determination of the differentiation capacities of murines' primary mononucleated cells and MC3T3-E1 cells |
| title_full_unstemmed | Determination of the differentiation capacities of murines' primary mononucleated cells and MC3T3-E1 cells |
| title_short | Determination of the differentiation capacities of murines' primary mononucleated cells and MC3T3-E1 cells |
| title_sort | determination of the differentiation capacities of murines primary mononucleated cells and mc3t3 e1 cells |
| url | http://www.cancerci.com/content/10/1/42 |
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