Determination of Verification Parameters for Using the Manual Sampling Device for Fresh Raw Beef Trim
Multifaceted food safety systems are used by the beef processing industry to minimize risk of bacterial contamination of the finished product. These systems are comprised of several parts including the conditional release of product requiring a sample to produce a negative result on a pathogen test...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-02-01
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| Series: | Journal of Food Protection |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0362028X23062440 |
| _version_ | 1811153956914593792 |
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| author | Terrance M. Arthur Ted Brown Tommy L. Wheeler |
| author_facet | Terrance M. Arthur Ted Brown Tommy L. Wheeler |
| author_sort | Terrance M. Arthur |
| collection | DOAJ |
| description | Multifaceted food safety systems are used by the beef processing industry to minimize risk of bacterial contamination of the finished product. These systems are comprised of several parts including the conditional release of product requiring a sample to produce a negative result on a pathogen test prior to sending the product into the food supply. The methods of sample collection require verification activities that ensure the sampling protocols are performed adequately. The research described herein was done to determine the parameters for use in verifying adequate beef trim sampling for the Manual Sampling Device (MSD) method. In addition, the efficacy of repeated sampling via multiple applications of the MSD procedure on a fresh raw beef trim combo was investigated. The results show that MSD sample collection that covers less than the entire combo surface, but at least one-half of the combo surface and is collected for a minimum of 90 s, is adequate for the recovery of organisms and prevalence targets from fresh raw beef trim. In addition, the evidence that MSD sample collection that occurs for less than the recommended time, but not less than 30 s per side of the swab, is adequate for the recovery of organisms and prevalence targets from raw beef trim. Finally, results show that in a scenario where an in-plant MSD sample and a regulatory MSD sample are required from the same combo, two MSD samples can be collected from the same combo bin with similar test results for both samples. While the recommended MSD protocol specifications will not be changed, the data presented herein provide support for some flexibility in accepting test results when verification activities indicate that sampling did not occur as specified in the recommended procedure. |
| first_indexed | 2024-03-13T01:19:35Z |
| format | Article |
| id | doaj.art-85516de69ad742d0978f1992e4165810 |
| institution | Directory Open Access Journal |
| issn | 0362-028X |
| language | English |
| last_indexed | 2024-03-13T01:19:35Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Food Protection |
| spelling | doaj.art-85516de69ad742d0978f1992e41658102023-07-05T05:13:54ZengElsevierJournal of Food Protection0362-028X2023-02-01862100041Determination of Verification Parameters for Using the Manual Sampling Device for Fresh Raw Beef TrimTerrance M. Arthur0Ted Brown1Tommy L. Wheeler2U.S. Department of Agriculture, Agricultural Research Service, Roman L. Hruska U. S. Meat Animal Research Center, Clay Center, NE 68933, USA; Corresponding author. Present address: Meat Safety and Quality Research Unit, U.S. Meat Animal Research Center, Agricultural Research Service, USDA, PO Box 166, State Spur 18 D, Clay Center, NE 68933, USA.Cargill Inc, Wichita, KS 67202, USAU.S. Department of Agriculture, Agricultural Research Service, Roman L. Hruska U. S. Meat Animal Research Center, Clay Center, NE 68933, USAMultifaceted food safety systems are used by the beef processing industry to minimize risk of bacterial contamination of the finished product. These systems are comprised of several parts including the conditional release of product requiring a sample to produce a negative result on a pathogen test prior to sending the product into the food supply. The methods of sample collection require verification activities that ensure the sampling protocols are performed adequately. The research described herein was done to determine the parameters for use in verifying adequate beef trim sampling for the Manual Sampling Device (MSD) method. In addition, the efficacy of repeated sampling via multiple applications of the MSD procedure on a fresh raw beef trim combo was investigated. The results show that MSD sample collection that covers less than the entire combo surface, but at least one-half of the combo surface and is collected for a minimum of 90 s, is adequate for the recovery of organisms and prevalence targets from fresh raw beef trim. In addition, the evidence that MSD sample collection that occurs for less than the recommended time, but not less than 30 s per side of the swab, is adequate for the recovery of organisms and prevalence targets from raw beef trim. Finally, results show that in a scenario where an in-plant MSD sample and a regulatory MSD sample are required from the same combo, two MSD samples can be collected from the same combo bin with similar test results for both samples. While the recommended MSD protocol specifications will not be changed, the data presented herein provide support for some flexibility in accepting test results when verification activities indicate that sampling did not occur as specified in the recommended procedure.http://www.sciencedirect.com/science/article/pii/S0362028X23062440Beef trimIndex organismsMicro TallyMSDPathogenVerification |
| spellingShingle | Terrance M. Arthur Ted Brown Tommy L. Wheeler Determination of Verification Parameters for Using the Manual Sampling Device for Fresh Raw Beef Trim Journal of Food Protection Beef trim Index organisms Micro Tally MSD Pathogen Verification |
| title | Determination of Verification Parameters for Using the Manual Sampling Device for Fresh Raw Beef Trim |
| title_full | Determination of Verification Parameters for Using the Manual Sampling Device for Fresh Raw Beef Trim |
| title_fullStr | Determination of Verification Parameters for Using the Manual Sampling Device for Fresh Raw Beef Trim |
| title_full_unstemmed | Determination of Verification Parameters for Using the Manual Sampling Device for Fresh Raw Beef Trim |
| title_short | Determination of Verification Parameters for Using the Manual Sampling Device for Fresh Raw Beef Trim |
| title_sort | determination of verification parameters for using the manual sampling device for fresh raw beef trim |
| topic | Beef trim Index organisms Micro Tally MSD Pathogen Verification |
| url | http://www.sciencedirect.com/science/article/pii/S0362028X23062440 |
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