Flow cytometric immunophenotyping and cell block immunocytochemistry in the diagnosis of primary Non-Hodgkin′s Lymphoma by fine-needle aspiration: Experience from a tertiary care center
Background: Accurate diagnosis of Non-Hodgkin′s Lymphoma (NHL) on fine-needle aspiration (FNA) specimen is challenging and requires ancillary testing. Aim: The feasibility of flow cytometric immunophenotyping (FCI) along with cell block immunocytochemistry (CB-ICC) as adjunct techniques in the diag...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-01-01
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| Series: | Journal of Cytology |
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| Online Access: | http://www.jcytol.org/article.asp?issn=0970-9371;year=2014;volume=31;issue=3;spage=123;epage=130;aulast=Paul |
| _version_ | 1818792181827108864 |
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| author | Tuhin Paul Upasana Gautam Arvind Rajwanshi Ashim Das Amita Trehan Pankaj Malhotra Radhika Srinivasan |
| author_facet | Tuhin Paul Upasana Gautam Arvind Rajwanshi Ashim Das Amita Trehan Pankaj Malhotra Radhika Srinivasan |
| author_sort | Tuhin Paul |
| collection | DOAJ |
| description | Background: Accurate diagnosis of Non-Hodgkin′s Lymphoma (NHL) on fine-needle aspiration (FNA) specimen is challenging and requires ancillary testing.
Aim: The feasibility of flow cytometric immunophenotyping (FCI) along with cell block immunocytochemistry (CB-ICC) as adjunct techniques in the diagnosis of NHL as per the current World Health Organization (WHO) classification was evaluated.
Materials and Methods: All cases of suspected lymphoma underwent FNA, and the sample was triaged for light microscopic evaluation, FCI, and CB-ICC, and each case was classified as per the current WHO classification.
Results: A total of 65 cases was analyzed which included 40 B-cell, 21 T-cell, and 4 unclassifiable lymphomas. Of 61 cases, FCI alone was contributory in 74% (45/61) cases whereas CB-ICC alone was contributory in 65.5% (40/61) cases in typing the lymphoma. In 11.4% (7/61) cases, the lymphoma could not be classified by either technique. Thus, in a total of 88.5% (54/61) cases a combination of FCI and CB-ICC from FNA enabled a diagnosis of lymphoma with its subtyping.
Conclusion: Flow cytometric immunophenotyping and ICC on CBs are feasible on FNA material and are very useful in a suspected case of NHL especially when a biopsy may not be possible or feasible. |
| first_indexed | 2024-12-18T15:23:10Z |
| format | Article |
| id | doaj.art-85528f20b2064c7495c8e43f8ca641e4 |
| institution | Directory Open Access Journal |
| issn | 0970-9371 |
| language | English |
| last_indexed | 2024-12-18T15:23:10Z |
| publishDate | 2014-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Cytology |
| spelling | doaj.art-85528f20b2064c7495c8e43f8ca641e42022-12-21T21:03:20ZengWolters Kluwer Medknow PublicationsJournal of Cytology0970-93712014-01-0131312313010.4103/0970-9371.145577Flow cytometric immunophenotyping and cell block immunocytochemistry in the diagnosis of primary Non-Hodgkin′s Lymphoma by fine-needle aspiration: Experience from a tertiary care centerTuhin PaulUpasana GautamArvind RajwanshiAshim DasAmita TrehanPankaj MalhotraRadhika SrinivasanBackground: Accurate diagnosis of Non-Hodgkin′s Lymphoma (NHL) on fine-needle aspiration (FNA) specimen is challenging and requires ancillary testing. Aim: The feasibility of flow cytometric immunophenotyping (FCI) along with cell block immunocytochemistry (CB-ICC) as adjunct techniques in the diagnosis of NHL as per the current World Health Organization (WHO) classification was evaluated. Materials and Methods: All cases of suspected lymphoma underwent FNA, and the sample was triaged for light microscopic evaluation, FCI, and CB-ICC, and each case was classified as per the current WHO classification. Results: A total of 65 cases was analyzed which included 40 B-cell, 21 T-cell, and 4 unclassifiable lymphomas. Of 61 cases, FCI alone was contributory in 74% (45/61) cases whereas CB-ICC alone was contributory in 65.5% (40/61) cases in typing the lymphoma. In 11.4% (7/61) cases, the lymphoma could not be classified by either technique. Thus, in a total of 88.5% (54/61) cases a combination of FCI and CB-ICC from FNA enabled a diagnosis of lymphoma with its subtyping. Conclusion: Flow cytometric immunophenotyping and ICC on CBs are feasible on FNA material and are very useful in a suspected case of NHL especially when a biopsy may not be possible or feasible.http://www.jcytol.org/article.asp?issn=0970-9371;year=2014;volume=31;issue=3;spage=123;epage=130;aulast=PaulCell block; fine-needle aspiration cytology; flow cytometric immunophenotyping; flow cytometry; immunohistochemistry; lymphoma; World Health Organization classification |
| spellingShingle | Tuhin Paul Upasana Gautam Arvind Rajwanshi Ashim Das Amita Trehan Pankaj Malhotra Radhika Srinivasan Flow cytometric immunophenotyping and cell block immunocytochemistry in the diagnosis of primary Non-Hodgkin′s Lymphoma by fine-needle aspiration: Experience from a tertiary care center Journal of Cytology Cell block; fine-needle aspiration cytology; flow cytometric immunophenotyping; flow cytometry; immunohistochemistry; lymphoma; World Health Organization classification |
| title | Flow cytometric immunophenotyping and cell block immunocytochemistry in the diagnosis of primary Non-Hodgkin′s Lymphoma by fine-needle aspiration: Experience from a tertiary care center |
| title_full | Flow cytometric immunophenotyping and cell block immunocytochemistry in the diagnosis of primary Non-Hodgkin′s Lymphoma by fine-needle aspiration: Experience from a tertiary care center |
| title_fullStr | Flow cytometric immunophenotyping and cell block immunocytochemistry in the diagnosis of primary Non-Hodgkin′s Lymphoma by fine-needle aspiration: Experience from a tertiary care center |
| title_full_unstemmed | Flow cytometric immunophenotyping and cell block immunocytochemistry in the diagnosis of primary Non-Hodgkin′s Lymphoma by fine-needle aspiration: Experience from a tertiary care center |
| title_short | Flow cytometric immunophenotyping and cell block immunocytochemistry in the diagnosis of primary Non-Hodgkin′s Lymphoma by fine-needle aspiration: Experience from a tertiary care center |
| title_sort | flow cytometric immunophenotyping and cell block immunocytochemistry in the diagnosis of primary non hodgkin s lymphoma by fine needle aspiration experience from a tertiary care center |
| topic | Cell block; fine-needle aspiration cytology; flow cytometric immunophenotyping; flow cytometry; immunohistochemistry; lymphoma; World Health Organization classification |
| url | http://www.jcytol.org/article.asp?issn=0970-9371;year=2014;volume=31;issue=3;spage=123;epage=130;aulast=Paul |
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