New methods of microbiological identification using MALDI-TOF
Rapid diagnosis of pathogens is decisive to guarantee adequate therapy in infections: culture methods are precise and sensitive, but rather slow. New resources are available to enable faster diagnosis, and the most promising is MALDI-TOF technology: mass spectrometry applied to microbiological diagn...
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| Format: | Article |
| Language: | English |
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Instituto Israelita de Ensino e Pesquisa Albert Einstein
2012-03-01
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| Series: | Einstein (São Paulo) |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082012000100026&tlng=en |
| _version_ | 1818743805325606912 |
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| author | Jacyr Pasternak |
| author_facet | Jacyr Pasternak |
| author_sort | Jacyr Pasternak |
| collection | DOAJ |
| description | Rapid diagnosis of pathogens is decisive to guarantee adequate therapy in infections: culture methods are precise and sensitive, but rather slow. New resources are available to enable faster diagnosis, and the most promising is MALDI-TOF technology: mass spectrometry applied to microbiological diagnosis. Times as fast as 10 to 15 minutes to etiological diagnosis are possible after a positive blood culture result. We hope to have this technology in our laboratory, ANVISA permitting and improving their very slow rate of doing things... MALDI-TOF is basically putting a sample of culture or an enriched suspension of the probable pathogen over a small spot with a matrix and vaporizing it with a laser pulse: the products are aspired into a chamber, ionized and reach detectors at variable times: the detectors show time of arrival and quantity of the product, and each pathogen has its characteristic spectrum analyzed by a software. |
| first_indexed | 2024-12-18T02:34:15Z |
| format | Article |
| id | doaj.art-8555f719bf044054b43de82c3b76d452 |
| institution | Directory Open Access Journal |
| issn | 2317-6385 |
| language | English |
| last_indexed | 2024-12-18T02:34:15Z |
| publishDate | 2012-03-01 |
| publisher | Instituto Israelita de Ensino e Pesquisa Albert Einstein |
| record_format | Article |
| series | Einstein (São Paulo) |
| spelling | doaj.art-8555f719bf044054b43de82c3b76d4522022-12-21T21:23:49ZengInstituto Israelita de Ensino e Pesquisa Albert EinsteinEinstein (São Paulo)2317-63852012-03-01101118119New methods of microbiological identification using MALDI-TOFJacyr Pasternak0Hospital Israelita Albert EinsteinRapid diagnosis of pathogens is decisive to guarantee adequate therapy in infections: culture methods are precise and sensitive, but rather slow. New resources are available to enable faster diagnosis, and the most promising is MALDI-TOF technology: mass spectrometry applied to microbiological diagnosis. Times as fast as 10 to 15 minutes to etiological diagnosis are possible after a positive blood culture result. We hope to have this technology in our laboratory, ANVISA permitting and improving their very slow rate of doing things... MALDI-TOF is basically putting a sample of culture or an enriched suspension of the probable pathogen over a small spot with a matrix and vaporizing it with a laser pulse: the products are aspired into a chamber, ionized and reach detectors at variable times: the detectors show time of arrival and quantity of the product, and each pathogen has its characteristic spectrum analyzed by a software.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082012000100026&tlng=enSpectrometry, mass, matrix-assisted laser desorption-ionization |
| spellingShingle | Jacyr Pasternak New methods of microbiological identification using MALDI-TOF Einstein (São Paulo) Spectrometry, mass, matrix-assisted laser desorption-ionization |
| title | New methods of microbiological identification using MALDI-TOF |
| title_full | New methods of microbiological identification using MALDI-TOF |
| title_fullStr | New methods of microbiological identification using MALDI-TOF |
| title_full_unstemmed | New methods of microbiological identification using MALDI-TOF |
| title_short | New methods of microbiological identification using MALDI-TOF |
| title_sort | new methods of microbiological identification using maldi tof |
| topic | Spectrometry, mass, matrix-assisted laser desorption-ionization |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-45082012000100026&tlng=en |
| work_keys_str_mv | AT jacyrpasternak newmethodsofmicrobiologicalidentificationusingmalditof |