The potential immunomodulatory role of the gut microbiota in the pathogenesis of asthma: an in vitro study
Abstract The aim of this study was to investigate the influence of Bacteroides vulgatus (BV), Clostridium perfringens (CP), Parabacteroides distasonis (PD) and Ruminococcus albus (RA) lysates on secretion of selected cytokines by PBMC, MDM and HT-29 cells, as well as to determine the potential mecha...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-47003-0 |
| _version_ | 1797559981659127808 |
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| author | Paulina Kleniewska Paulina Natalia Kopa-Stojak Arkadiusz Hoffmann Rafał Pawliczak |
| author_facet | Paulina Kleniewska Paulina Natalia Kopa-Stojak Arkadiusz Hoffmann Rafał Pawliczak |
| author_sort | Paulina Kleniewska |
| collection | DOAJ |
| description | Abstract The aim of this study was to investigate the influence of Bacteroides vulgatus (BV), Clostridium perfringens (CP), Parabacteroides distasonis (PD) and Ruminococcus albus (RA) lysates on secretion of selected cytokines by PBMC, MDM and HT-29 cells, as well as to determine the potential mechanisms of their action in the development of asthma. Enzyme-linked immunosorbent assays were used to analyze the effect of BV, CP, PD and RA lysates on the secretion of IL-1β, IL-6, IL-10 and TNF-α by human PBMC, MDM and HT-29 cells. BV and CP lysates significantly lowered IL-1β secretion by MDM vs. control (p < 0.05 and p < 0.001 respectively) but only at a dose of 400 µg lysate. The secretions of IL-6 by PBMC and MDM were elevated significantly above control values (p < 0.05) after administration of CP and PD lysates. BV, CP and PD lysates (100 µg) significantly increased IL-10 secretion by PBMC vs. control (p < 0.05). CP, PD and RA lysates (400 µg) significantly increased IL-10 secretion by MDM vs. control (p < 0.001). BV lysate (400 µg) also significantly increased IL-10 secretion by MDM as compared to control (p < 0.05). In PBMC and MDM, the production levels of the anti-inflammatory cytokine were increased by all the bacterial lysates used in a dose-dependent manner. |
| first_indexed | 2024-03-10T17:53:55Z |
| format | Article |
| id | doaj.art-85578cc8120844d48ecb8806938e71fd |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-10T17:53:55Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-85578cc8120844d48ecb8806938e71fd2023-11-20T09:16:21ZengNature PortfolioScientific Reports2045-23222023-11-011311810.1038/s41598-023-47003-0The potential immunomodulatory role of the gut microbiota in the pathogenesis of asthma: an in vitro studyPaulina Kleniewska0Paulina Natalia Kopa-Stojak1Arkadiusz Hoffmann2Rafał Pawliczak3Department of Immunopathology, Faculty of Medicine, Medical University of LodzDepartment of Immunopathology, Faculty of Medicine, Medical University of LodzDepartment of Immunopathology, Faculty of Medicine, Medical University of LodzDepartment of Immunopathology, Faculty of Medicine, Medical University of LodzAbstract The aim of this study was to investigate the influence of Bacteroides vulgatus (BV), Clostridium perfringens (CP), Parabacteroides distasonis (PD) and Ruminococcus albus (RA) lysates on secretion of selected cytokines by PBMC, MDM and HT-29 cells, as well as to determine the potential mechanisms of their action in the development of asthma. Enzyme-linked immunosorbent assays were used to analyze the effect of BV, CP, PD and RA lysates on the secretion of IL-1β, IL-6, IL-10 and TNF-α by human PBMC, MDM and HT-29 cells. BV and CP lysates significantly lowered IL-1β secretion by MDM vs. control (p < 0.05 and p < 0.001 respectively) but only at a dose of 400 µg lysate. The secretions of IL-6 by PBMC and MDM were elevated significantly above control values (p < 0.05) after administration of CP and PD lysates. BV, CP and PD lysates (100 µg) significantly increased IL-10 secretion by PBMC vs. control (p < 0.05). CP, PD and RA lysates (400 µg) significantly increased IL-10 secretion by MDM vs. control (p < 0.001). BV lysate (400 µg) also significantly increased IL-10 secretion by MDM as compared to control (p < 0.05). In PBMC and MDM, the production levels of the anti-inflammatory cytokine were increased by all the bacterial lysates used in a dose-dependent manner.https://doi.org/10.1038/s41598-023-47003-0 |
| spellingShingle | Paulina Kleniewska Paulina Natalia Kopa-Stojak Arkadiusz Hoffmann Rafał Pawliczak The potential immunomodulatory role of the gut microbiota in the pathogenesis of asthma: an in vitro study Scientific Reports |
| title | The potential immunomodulatory role of the gut microbiota in the pathogenesis of asthma: an in vitro study |
| title_full | The potential immunomodulatory role of the gut microbiota in the pathogenesis of asthma: an in vitro study |
| title_fullStr | The potential immunomodulatory role of the gut microbiota in the pathogenesis of asthma: an in vitro study |
| title_full_unstemmed | The potential immunomodulatory role of the gut microbiota in the pathogenesis of asthma: an in vitro study |
| title_short | The potential immunomodulatory role of the gut microbiota in the pathogenesis of asthma: an in vitro study |
| title_sort | potential immunomodulatory role of the gut microbiota in the pathogenesis of asthma an in vitro study |
| url | https://doi.org/10.1038/s41598-023-47003-0 |
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