Unusual Long-Distance Movement Strategies of Potato mop-top virus RNAs in Nicotiana benthamiana
The Potato mop-top virus (PMTV) genome encodes replicase, movement, and capsid proteins on three different RNA species that are encapsidated within tubular rod-shaped particles. Previously, we showed that the protein produced on translational readthrough (RT) of the coat protein (CP) gene, CP-RT, is...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2009-04-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-4-0381 |
| _version_ | 1811277629942136832 |
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| author | Lesley Torrance Nina I. Lukhovitskaya Mikhail V. Schepetilnikov Graham H. Cowan Angelika Ziegler Eugene I. Savenkov |
| author_facet | Lesley Torrance Nina I. Lukhovitskaya Mikhail V. Schepetilnikov Graham H. Cowan Angelika Ziegler Eugene I. Savenkov |
| author_sort | Lesley Torrance |
| collection | DOAJ |
| description | The Potato mop-top virus (PMTV) genome encodes replicase, movement, and capsid proteins on three different RNA species that are encapsidated within tubular rod-shaped particles. Previously, we showed that the protein produced on translational readthrough (RT) of the coat protein (CP) gene, CP-RT, is associated with one extremity of the virus particles, and that the two RNAs encoding replicase and movement proteins can move long distance in the absence of the third RNA (RNA-CP) that encodes the capsid proteins, CP and CP-RT. Here, we examined the roles of the CP and CP-RT proteins on RNA movement using infectious clones carrying mutations in the CP and CP-RT coding domains. The results showed that, in infections established with mutant RNA-CP expressing CP together with truncated CP-RT, systemic movement of the mutant RNA-CP was inhibited but not the movement of the other two RNAs. Furthermore, RNA-CP long-distance movement was inhibited in a mutant clone expressing only CP in the absence of the CP-RT polypeptide. CP-RT was not necessary for particle assembly because virions were observed in leaf extracts infected with the CP-RT deletion mutants. RNA-CP moved long distance when protein expression was suppressed completely or when CP expression was suppressed so that only CP-RT or truncated CP-RT was expressed. CP-RT but not CP interacted with the movement protein TGB1 in the yeast two-hybrid system. CP-RT and TGB1 were detected by enzyme-linked immunosorbent assay in virus particles and the long-distance movement of RNA-CP was correlated with expression of CP-RT that interacted with TGB1; mutant RNA-CP expressing truncated CP-RT proteins that did not interact with TGB1 formed virions but did not move to upper noninoculated leaves. The results indicate that PMTV RNA-CP can move long distance in two distinct forms: either as a viral ribonucleoprotein complex or as particles that are most likely associated with CP-RT and TGB1. |
| first_indexed | 2024-04-13T00:20:17Z |
| format | Article |
| id | doaj.art-8557f1a57a6146c0ad87e03f6dcf03aa |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T00:20:17Z |
| publishDate | 2009-04-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-8557f1a57a6146c0ad87e03f6dcf03aa2022-12-22T03:10:48ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062009-04-0122438139010.1094/MPMI-22-4-0381Unusual Long-Distance Movement Strategies of Potato mop-top virus RNAs in Nicotiana benthamianaLesley TorranceNina I. LukhovitskayaMikhail V. SchepetilnikovGraham H. CowanAngelika ZieglerEugene I. SavenkovThe Potato mop-top virus (PMTV) genome encodes replicase, movement, and capsid proteins on three different RNA species that are encapsidated within tubular rod-shaped particles. Previously, we showed that the protein produced on translational readthrough (RT) of the coat protein (CP) gene, CP-RT, is associated with one extremity of the virus particles, and that the two RNAs encoding replicase and movement proteins can move long distance in the absence of the third RNA (RNA-CP) that encodes the capsid proteins, CP and CP-RT. Here, we examined the roles of the CP and CP-RT proteins on RNA movement using infectious clones carrying mutations in the CP and CP-RT coding domains. The results showed that, in infections established with mutant RNA-CP expressing CP together with truncated CP-RT, systemic movement of the mutant RNA-CP was inhibited but not the movement of the other two RNAs. Furthermore, RNA-CP long-distance movement was inhibited in a mutant clone expressing only CP in the absence of the CP-RT polypeptide. CP-RT was not necessary for particle assembly because virions were observed in leaf extracts infected with the CP-RT deletion mutants. RNA-CP moved long distance when protein expression was suppressed completely or when CP expression was suppressed so that only CP-RT or truncated CP-RT was expressed. CP-RT but not CP interacted with the movement protein TGB1 in the yeast two-hybrid system. CP-RT and TGB1 were detected by enzyme-linked immunosorbent assay in virus particles and the long-distance movement of RNA-CP was correlated with expression of CP-RT that interacted with TGB1; mutant RNA-CP expressing truncated CP-RT proteins that did not interact with TGB1 formed virions but did not move to upper noninoculated leaves. The results indicate that PMTV RNA-CP can move long distance in two distinct forms: either as a viral ribonucleoprotein complex or as particles that are most likely associated with CP-RT and TGB1.https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-4-0381 |
| spellingShingle | Lesley Torrance Nina I. Lukhovitskaya Mikhail V. Schepetilnikov Graham H. Cowan Angelika Ziegler Eugene I. Savenkov Unusual Long-Distance Movement Strategies of Potato mop-top virus RNAs in Nicotiana benthamiana Molecular Plant-Microbe Interactions |
| title | Unusual Long-Distance Movement Strategies of Potato mop-top virus RNAs in Nicotiana benthamiana |
| title_full | Unusual Long-Distance Movement Strategies of Potato mop-top virus RNAs in Nicotiana benthamiana |
| title_fullStr | Unusual Long-Distance Movement Strategies of Potato mop-top virus RNAs in Nicotiana benthamiana |
| title_full_unstemmed | Unusual Long-Distance Movement Strategies of Potato mop-top virus RNAs in Nicotiana benthamiana |
| title_short | Unusual Long-Distance Movement Strategies of Potato mop-top virus RNAs in Nicotiana benthamiana |
| title_sort | unusual long distance movement strategies of potato mop top virus rnas in nicotiana benthamiana |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-22-4-0381 |
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