Controlled and Local Delivery of Antibiotics by 3D Core/Shell Printed Hydrogel Scaffolds to Treat Soft Tissue Infections
Soft tissue infections in open fractures or burns are major cause for high morbidity in trauma patients. Sustained, long-term and localized delivery of antimicrobial agents is needed for early eradication of these infections. Traditional (topical or systemic) antibiotic delivery methods are associat...
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MDPI AG
2021-12-01
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Series: | Pharmaceutics |
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Online Access: | https://www.mdpi.com/1999-4923/13/12/2151 |
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author | Ashwini Rahul Akkineni Janina Spangenberg Michael Geissler Saskia Reichelt Hubert Buechner Anja Lode Michael Gelinsky |
author_facet | Ashwini Rahul Akkineni Janina Spangenberg Michael Geissler Saskia Reichelt Hubert Buechner Anja Lode Michael Gelinsky |
author_sort | Ashwini Rahul Akkineni |
collection | DOAJ |
description | Soft tissue infections in open fractures or burns are major cause for high morbidity in trauma patients. Sustained, long-term and localized delivery of antimicrobial agents is needed for early eradication of these infections. Traditional (topical or systemic) antibiotic delivery methods are associated with a variety of problems, including their long-term unavailability and possible low local concentration. Novel approaches for antibiotic delivery via wound coverage/healing scaffolds are constantly being developed. Many of these approaches are associated with burst release and thus seldom maintain long-term inhibitory concentrations. Using 3D core/shell extrusion printing, scaffolds consisting of antibiotic depot (in the core composed of low concentrated biomaterial ink 3% alginate) surrounded by a denser biomaterial ink (shell) were fabricated. Denser biomaterial ink (composed of alginate and methylcellulose or alginate, methylcellulose and Laponite) retained scaffold shape and modulated antibiotic release kinetics. Release of antibiotics was observed over seven days, indicating sustained release characteristics and maintenance of potency. Inclusion of Laponite in shell, significantly reduced burst release of antibiotics. Additionally, the effect of shell thickness on release kinetics was demonstrated. Amalgamation of such a modular delivery system with other biofabrication methods could potentially open new strategies to simultaneously treat soft tissue infections and aid wound regeneration. |
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format | Article |
id | doaj.art-85582ec800ee49ce91e5d22fbdce6cd8 |
institution | Directory Open Access Journal |
issn | 1999-4923 |
language | English |
last_indexed | 2024-03-10T03:19:34Z |
publishDate | 2021-12-01 |
publisher | MDPI AG |
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series | Pharmaceutics |
spelling | doaj.art-85582ec800ee49ce91e5d22fbdce6cd82023-11-23T10:06:35ZengMDPI AGPharmaceutics1999-49232021-12-011312215110.3390/pharmaceutics13122151Controlled and Local Delivery of Antibiotics by 3D Core/Shell Printed Hydrogel Scaffolds to Treat Soft Tissue InfectionsAshwini Rahul Akkineni0Janina Spangenberg1Michael Geissler2Saskia Reichelt3Hubert Buechner4Anja Lode5Michael Gelinsky6Centre for Translational Bone, Joint and Soft Tissue Research, University Hospital Carl Gustav Carus, Faculty of Medicine of Technische Universität Dresden, 01307 Dresden, GermanyCentre for Translational Bone, Joint and Soft Tissue Research, University Hospital Carl Gustav Carus, Faculty of Medicine of Technische Universität Dresden, 01307 Dresden, GermanyCentre for Translational Bone, Joint and Soft Tissue Research, University Hospital Carl Gustav Carus, Faculty of Medicine of Technische Universität Dresden, 01307 Dresden, GermanyInstitute of Natural Materials Technology, Technische Universität Dresden, 01069 Dresden, GermanyHeraeus Medical GmbH, 61273 Wehrheim, GermanyCentre for Translational Bone, Joint and Soft Tissue Research, University Hospital Carl Gustav Carus, Faculty of Medicine of Technische Universität Dresden, 01307 Dresden, GermanyCentre for Translational Bone, Joint and Soft Tissue Research, University Hospital Carl Gustav Carus, Faculty of Medicine of Technische Universität Dresden, 01307 Dresden, GermanySoft tissue infections in open fractures or burns are major cause for high morbidity in trauma patients. Sustained, long-term and localized delivery of antimicrobial agents is needed for early eradication of these infections. Traditional (topical or systemic) antibiotic delivery methods are associated with a variety of problems, including their long-term unavailability and possible low local concentration. Novel approaches for antibiotic delivery via wound coverage/healing scaffolds are constantly being developed. Many of these approaches are associated with burst release and thus seldom maintain long-term inhibitory concentrations. Using 3D core/shell extrusion printing, scaffolds consisting of antibiotic depot (in the core composed of low concentrated biomaterial ink 3% alginate) surrounded by a denser biomaterial ink (shell) were fabricated. Denser biomaterial ink (composed of alginate and methylcellulose or alginate, methylcellulose and Laponite) retained scaffold shape and modulated antibiotic release kinetics. Release of antibiotics was observed over seven days, indicating sustained release characteristics and maintenance of potency. Inclusion of Laponite in shell, significantly reduced burst release of antibiotics. Additionally, the effect of shell thickness on release kinetics was demonstrated. Amalgamation of such a modular delivery system with other biofabrication methods could potentially open new strategies to simultaneously treat soft tissue infections and aid wound regeneration.https://www.mdpi.com/1999-4923/13/12/21513D core/shell printingdrug deliveryantibioticshydrogelssoft tissue infection |
spellingShingle | Ashwini Rahul Akkineni Janina Spangenberg Michael Geissler Saskia Reichelt Hubert Buechner Anja Lode Michael Gelinsky Controlled and Local Delivery of Antibiotics by 3D Core/Shell Printed Hydrogel Scaffolds to Treat Soft Tissue Infections Pharmaceutics 3D core/shell printing drug delivery antibiotics hydrogels soft tissue infection |
title | Controlled and Local Delivery of Antibiotics by 3D Core/Shell Printed Hydrogel Scaffolds to Treat Soft Tissue Infections |
title_full | Controlled and Local Delivery of Antibiotics by 3D Core/Shell Printed Hydrogel Scaffolds to Treat Soft Tissue Infections |
title_fullStr | Controlled and Local Delivery of Antibiotics by 3D Core/Shell Printed Hydrogel Scaffolds to Treat Soft Tissue Infections |
title_full_unstemmed | Controlled and Local Delivery of Antibiotics by 3D Core/Shell Printed Hydrogel Scaffolds to Treat Soft Tissue Infections |
title_short | Controlled and Local Delivery of Antibiotics by 3D Core/Shell Printed Hydrogel Scaffolds to Treat Soft Tissue Infections |
title_sort | controlled and local delivery of antibiotics by 3d core shell printed hydrogel scaffolds to treat soft tissue infections |
topic | 3D core/shell printing drug delivery antibiotics hydrogels soft tissue infection |
url | https://www.mdpi.com/1999-4923/13/12/2151 |
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