Effects of rosiglitazone/PHBV drug delivery system on postoperative fibrosis in rabbit glaucoma filtration surgery model
The aim of this study is to investigate the effects and toxicities of poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV)-loading rosiglitazone on preventing scar formation after glaucoma filtration surgery (GFS) in the rabbit model. Rosiglitazone/PHBV drug delivery system was prepared via e...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Drug Delivery |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/10717544.2019.1648590 |
| _version_ | 1828894644268695552 |
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| author | Feng Zhang Ke Liu Zheng Pan Mengdan Cao Dengming Zhou Hairong Liu Yuting Huang Xuanchu Duan |
| author_facet | Feng Zhang Ke Liu Zheng Pan Mengdan Cao Dengming Zhou Hairong Liu Yuting Huang Xuanchu Duan |
| author_sort | Feng Zhang |
| collection | DOAJ |
| description | The aim of this study is to investigate the effects and toxicities of poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV)-loading rosiglitazone on preventing scar formation after glaucoma filtration surgery (GFS) in the rabbit model. Rosiglitazone/PHBV drug delivery system was prepared via electrospinning. Release behavior of RSG/PHBV membrane was evaluated by high-performance liquid chromatography. The different concentration membranes were implanted under the conjunctiva of the rabbit’s eyes (RSG/PHBV groups). Also, MMC-soaked sponges were placed under the conjunctiva of the eyes (positive group) for 3 min. Intraocular pressures and bleb features were then assessed for 4 weeks postoperative. Bleb sections were stained with HE, Masson’s trichrome and α smooth muscle action (αSMA) immunohistochemistry. The protein expression of collagen I, αSMA, and connective tissue growth factor in the bleb area were then quantified. The following results were observed: (1) the concentration of rosiglitazone would not affect the morphology of RSG/PHBV membrane. (2) RSG/PHBV membrane would effective and safety prevent the formation of fibrosis after GFS in the rabbit model. Implantation of RSG/PHBV membrane prevents scar formation after GFS. What’s more, it ameliorated toxicity to conjunctiva and cornea compared with the placement of MMC. The RSG/PHBV membrane would be a more effectivity and safer strategy than MMC. |
| first_indexed | 2024-12-13T14:15:15Z |
| format | Article |
| id | doaj.art-856290a302c348bdbc89864672ac9336 |
| institution | Directory Open Access Journal |
| issn | 1071-7544 1521-0464 |
| language | English |
| last_indexed | 2024-12-13T14:15:15Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Drug Delivery |
| spelling | doaj.art-856290a302c348bdbc89864672ac93362022-12-21T23:42:21ZengTaylor & Francis GroupDrug Delivery1071-75441521-04642019-01-0126181281910.1080/10717544.2019.16485901648590Effects of rosiglitazone/PHBV drug delivery system on postoperative fibrosis in rabbit glaucoma filtration surgery modelFeng Zhang0Ke Liu1Zheng Pan2Mengdan Cao3Dengming Zhou4Hairong Liu5Yuting Huang6Xuanchu Duan7The Second Xiangya Hospital, Central South UniversityThe Second Xiangya Hospital, Central South UniversityThe Second Xiangya Hospital, Central South UniversityThe Second Xiangya Hospital, Central South UniversityThe Second Xiangya Hospital, Central South UniversityHunan UniversityHunan UniversityThe Second Xiangya Hospital, Central South UniversityThe aim of this study is to investigate the effects and toxicities of poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV)-loading rosiglitazone on preventing scar formation after glaucoma filtration surgery (GFS) in the rabbit model. Rosiglitazone/PHBV drug delivery system was prepared via electrospinning. Release behavior of RSG/PHBV membrane was evaluated by high-performance liquid chromatography. The different concentration membranes were implanted under the conjunctiva of the rabbit’s eyes (RSG/PHBV groups). Also, MMC-soaked sponges were placed under the conjunctiva of the eyes (positive group) for 3 min. Intraocular pressures and bleb features were then assessed for 4 weeks postoperative. Bleb sections were stained with HE, Masson’s trichrome and α smooth muscle action (αSMA) immunohistochemistry. The protein expression of collagen I, αSMA, and connective tissue growth factor in the bleb area were then quantified. The following results were observed: (1) the concentration of rosiglitazone would not affect the morphology of RSG/PHBV membrane. (2) RSG/PHBV membrane would effective and safety prevent the formation of fibrosis after GFS in the rabbit model. Implantation of RSG/PHBV membrane prevents scar formation after GFS. What’s more, it ameliorated toxicity to conjunctiva and cornea compared with the placement of MMC. The RSG/PHBV membrane would be a more effectivity and safer strategy than MMC.http://dx.doi.org/10.1080/10717544.2019.1648590drug delivery systemphbvrosiglitazoneglaucoma filtration surgery |
| spellingShingle | Feng Zhang Ke Liu Zheng Pan Mengdan Cao Dengming Zhou Hairong Liu Yuting Huang Xuanchu Duan Effects of rosiglitazone/PHBV drug delivery system on postoperative fibrosis in rabbit glaucoma filtration surgery model Drug Delivery drug delivery system phbv rosiglitazone glaucoma filtration surgery |
| title | Effects of rosiglitazone/PHBV drug delivery system on postoperative fibrosis in rabbit glaucoma filtration surgery model |
| title_full | Effects of rosiglitazone/PHBV drug delivery system on postoperative fibrosis in rabbit glaucoma filtration surgery model |
| title_fullStr | Effects of rosiglitazone/PHBV drug delivery system on postoperative fibrosis in rabbit glaucoma filtration surgery model |
| title_full_unstemmed | Effects of rosiglitazone/PHBV drug delivery system on postoperative fibrosis in rabbit glaucoma filtration surgery model |
| title_short | Effects of rosiglitazone/PHBV drug delivery system on postoperative fibrosis in rabbit glaucoma filtration surgery model |
| title_sort | effects of rosiglitazone phbv drug delivery system on postoperative fibrosis in rabbit glaucoma filtration surgery model |
| topic | drug delivery system phbv rosiglitazone glaucoma filtration surgery |
| url | http://dx.doi.org/10.1080/10717544.2019.1648590 |
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