Identification of a CE-SDS shoulder peak as disulfide-linked fragments from common CH2 cleavages in IgGs and IgG-like bispecific antibodies

Identification of a CE-SDS shoulder peak as disulfide-linked fragments from common CH2 cleavages in IgGs and IgG-like bispecific antibodies

Fragmentation is a well-characterized degradation pathway of therapeutic antibodies and is usually monitored by capillary electrophoresis–sodium dodecyl sulfate (CE-SDS). Although fragments due to cleavage in CH2 domains linked by intrachain disulfide bonds are common and can be detected by reduced...

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Bibliographic Details
Main Authors: Mingyan Cao, Yang Jiao, Conner Parthemore, Samuel Korman, Jiao Ma, Alan Hunter, Greg Kilby, Xiaoyu Chen
Format: Article
Language:English
Published: Taylor & Francis Group 2021-01-01
Series:mAbs
Subjects:
IgG-like bispecific antibodies
monoclonal antibody
fragments
CE-SDS
hydrophobic interaction chromatography
intrachain disulfide bond
Online Access:https://www.tandfonline.com/doi/10.1080/19420862.2021.1981806

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