Let-7 family regulates HaCaT cell proliferation and apoptosis via the ΔNp63/PI3K/AKT pathway
We evaluated the expression profiles of differentially expressed miRNAs (DEmiRNAs) involved in human fetal skin development via high-throughput sequencing to explore the expression difference and the regulatory role of miRNA in different stages of fetal skin development. Analysis of expression profi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2024-03-01
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| Series: | Open Medicine |
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| Online Access: | https://doi.org/10.1515/med-2024-0925 |
| _version_ | 1797229664891043840 |
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| author | Li Min Ding Yi Tuersong Tayier Chen Long Zhang Mei-Lin Li Tian Feng Shu-Mei Guo Qiong |
| author_facet | Li Min Ding Yi Tuersong Tayier Chen Long Zhang Mei-Lin Li Tian Feng Shu-Mei Guo Qiong |
| author_sort | Li Min |
| collection | DOAJ |
| description | We evaluated the expression profiles of differentially expressed miRNAs (DEmiRNAs) involved in human fetal skin development via high-throughput sequencing to explore the expression difference and the regulatory role of miRNA in different stages of fetal skin development. Analysis of expression profiles of miRNAs involved collecting embryo samples via high-throughput sequencing, then bioinformatics analyses were performed to validate DEmiRNAs. A total of 363 miRNAs were differentially expressed during the early and mid-pregnancy of development, and upregulated DEmiRNAs were mainly concentrated in the let-7 family. The transfection of let-7b-5p slowed down HaCaT cell proliferation and promoted apoptosis, as evidenced by the cell counting kit-8 assay, quantitative real-time polymerase chain reaction, and flow cytometry. The double luciferin reporter assay also confirmed let-7b-5p and ΔNp63 downregulation through the combination with the 3ʹ-untranslated region of ΔNp63. Moreover, treatment with a let-7b-5p inhibitor upregulated ΔNp63 and activated the phosphoinositide 3-kinase (PI3K)-protein kinase B (AKT) signaling pathway. The let-7b-5p caused a converse effect on HaCaT cells because of Np63 upregulation. Let-7b-5p regulates skin development by targeting ΔNp63 via PI3K-AKT signaling, contributing to future studies on skin development and clinical scar-free healing. |
| first_indexed | 2024-04-24T15:16:12Z |
| format | Article |
| id | doaj.art-8568b391cf6e4f5baca1176814bdaef1 |
| institution | Directory Open Access Journal |
| issn | 2391-5463 |
| language | English |
| last_indexed | 2024-04-24T15:16:12Z |
| publishDate | 2024-03-01 |
| publisher | De Gruyter |
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| series | Open Medicine |
| spelling | doaj.art-8568b391cf6e4f5baca1176814bdaef12024-04-02T09:20:26ZengDe GruyterOpen Medicine2391-54632024-03-01191e10412610.1515/med-2024-0925Let-7 family regulates HaCaT cell proliferation and apoptosis via the ΔNp63/PI3K/AKT pathwayLi Min0Ding Yi1Tuersong Tayier2Chen Long3Zhang Mei-Lin4Li Tian5Feng Shu-Mei6Guo Qiong7Department of Histology and Embryology, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi, 830000, Xinjiang, ChinaDepartment of Histology and Embryology, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi, 830000, Xinjiang, ChinaDepartment of Pharmacy, The Second Affiliated Hospital of Xinjiang Medical University, Urumqi, 830000, Xinjiang, ChinaFunctional Center, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi, 830000, Xinjiang, ChinaXinjiang Urumqi City Center Blood Station, Urumqi, 830000, Xinjiang, ChinaDepartment of Histology and Embryology, School of Basic Medical Sciences, Xinjiang Medical University, Urumqi, 830000, Xinjiang, ChinaDepartment of Histology and Embryology, School of Basic Medical Sciences, Xinjiang Medical University, No. 567 Suntech North Road, Shuimogou District, Urumqi, 830000, Xinjiang, ChinaDepartment of Histology and Embryology, School of Basic Medical Sciences, Xinjiang Medical University, No. 567 Suntech North Road, Shuimogou District, Urumqi, 830000, Xinjiang, ChinaWe evaluated the expression profiles of differentially expressed miRNAs (DEmiRNAs) involved in human fetal skin development via high-throughput sequencing to explore the expression difference and the regulatory role of miRNA in different stages of fetal skin development. Analysis of expression profiles of miRNAs involved collecting embryo samples via high-throughput sequencing, then bioinformatics analyses were performed to validate DEmiRNAs. A total of 363 miRNAs were differentially expressed during the early and mid-pregnancy of development, and upregulated DEmiRNAs were mainly concentrated in the let-7 family. The transfection of let-7b-5p slowed down HaCaT cell proliferation and promoted apoptosis, as evidenced by the cell counting kit-8 assay, quantitative real-time polymerase chain reaction, and flow cytometry. The double luciferin reporter assay also confirmed let-7b-5p and ΔNp63 downregulation through the combination with the 3ʹ-untranslated region of ΔNp63. Moreover, treatment with a let-7b-5p inhibitor upregulated ΔNp63 and activated the phosphoinositide 3-kinase (PI3K)-protein kinase B (AKT) signaling pathway. The let-7b-5p caused a converse effect on HaCaT cells because of Np63 upregulation. Let-7b-5p regulates skin development by targeting ΔNp63 via PI3K-AKT signaling, contributing to future studies on skin development and clinical scar-free healing.https://doi.org/10.1515/med-2024-0925fetal skinlet-7 familyδnp63scar-free skin healingphosphoinositide 3-kinase-protein kinase b signaling |
| spellingShingle | Li Min Ding Yi Tuersong Tayier Chen Long Zhang Mei-Lin Li Tian Feng Shu-Mei Guo Qiong Let-7 family regulates HaCaT cell proliferation and apoptosis via the ΔNp63/PI3K/AKT pathway Open Medicine fetal skin let-7 family δnp63 scar-free skin healing phosphoinositide 3-kinase-protein kinase b signaling |
| title | Let-7 family regulates HaCaT cell proliferation and apoptosis via the ΔNp63/PI3K/AKT pathway |
| title_full | Let-7 family regulates HaCaT cell proliferation and apoptosis via the ΔNp63/PI3K/AKT pathway |
| title_fullStr | Let-7 family regulates HaCaT cell proliferation and apoptosis via the ΔNp63/PI3K/AKT pathway |
| title_full_unstemmed | Let-7 family regulates HaCaT cell proliferation and apoptosis via the ΔNp63/PI3K/AKT pathway |
| title_short | Let-7 family regulates HaCaT cell proliferation and apoptosis via the ΔNp63/PI3K/AKT pathway |
| title_sort | let 7 family regulates hacat cell proliferation and apoptosis via the δnp63 pi3k akt pathway |
| topic | fetal skin let-7 family δnp63 scar-free skin healing phosphoinositide 3-kinase-protein kinase b signaling |
| url | https://doi.org/10.1515/med-2024-0925 |
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