Hybrid Nanomaterials of Magnetic Iron Nanoparticles and Graphene Oxide as Matrices for the Immobilization of β-Glucosidase: Synthesis, Characterization, and Biocatalytic Properties
Hybrid nanostructures of magnetic iron nanoparticles and graphene oxide were synthesized and used as nanosupports for the covalent immobilization of β-glucosidase. This study revealed that the immobilization efficiency depends on the structure and the surface chemistry of nanostructures employed. Th...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-04-01
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| Series: | Frontiers in Materials |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmats.2018.00025/full |
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| author | Georgios Orfanakis Michaela Patila Alexandra V. Catzikonstantinou Kyriaki-Marina Lyra Antonios Kouloumpis Konstantinos Spyrou Petros Katapodis Alkiviadis Paipetis Petra Rudolf Dimitrios Gournis Haralambos Stamatis |
| author_facet | Georgios Orfanakis Michaela Patila Alexandra V. Catzikonstantinou Kyriaki-Marina Lyra Antonios Kouloumpis Konstantinos Spyrou Petros Katapodis Alkiviadis Paipetis Petra Rudolf Dimitrios Gournis Haralambos Stamatis |
| author_sort | Georgios Orfanakis |
| collection | DOAJ |
| description | Hybrid nanostructures of magnetic iron nanoparticles and graphene oxide were synthesized and used as nanosupports for the covalent immobilization of β-glucosidase. This study revealed that the immobilization efficiency depends on the structure and the surface chemistry of nanostructures employed. The hybrid nanostructure-based biocatalysts formed exhibited a two to four-fold higher thermostability as compared to the free enzyme, as well as an enhanced performance at higher temperatures (up to 70°C) and in a wider pH range. Moreover, these biocatalysts retained a significant part of their bioactivity (up to 40%) after 12 repeated reaction cycles. |
| first_indexed | 2024-12-21T23:40:39Z |
| format | Article |
| id | doaj.art-85698c68bdf647b687069ea3f1977c14 |
| institution | Directory Open Access Journal |
| issn | 2296-8016 |
| language | English |
| last_indexed | 2024-12-21T23:40:39Z |
| publishDate | 2018-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Materials |
| spelling | doaj.art-85698c68bdf647b687069ea3f1977c142022-12-21T18:46:15ZengFrontiers Media S.A.Frontiers in Materials2296-80162018-04-01510.3389/fmats.2018.00025342573Hybrid Nanomaterials of Magnetic Iron Nanoparticles and Graphene Oxide as Matrices for the Immobilization of β-Glucosidase: Synthesis, Characterization, and Biocatalytic PropertiesGeorgios Orfanakis0Michaela Patila1Alexandra V. Catzikonstantinou2Kyriaki-Marina Lyra3Antonios Kouloumpis4Konstantinos Spyrou5Petros Katapodis6Alkiviadis Paipetis7Petra Rudolf8Dimitrios Gournis9Haralambos Stamatis10Biotechnology Laboratory, Department of Biological Applications and Technologies, University of Ioannina, Ioannina, GreeceBiotechnology Laboratory, Department of Biological Applications and Technologies, University of Ioannina, Ioannina, GreeceBiotechnology Laboratory, Department of Biological Applications and Technologies, University of Ioannina, Ioannina, GreeceDepartment of Material Science and Engineering, University of Ioannina, Ioannina, GreeceDepartment of Material Science and Engineering, University of Ioannina, Ioannina, GreeceDepartment of Material Science and Engineering, University of Ioannina, Ioannina, GreeceBiotechnology Laboratory, Department of Biological Applications and Technologies, University of Ioannina, Ioannina, GreeceDepartment of Material Science and Engineering, University of Ioannina, Ioannina, GreeceFaculty of Science and Engineering, Zernike Institute for Advanced Materials, University of Groningen, Groningen, NetherlandsDepartment of Material Science and Engineering, University of Ioannina, Ioannina, GreeceBiotechnology Laboratory, Department of Biological Applications and Technologies, University of Ioannina, Ioannina, GreeceHybrid nanostructures of magnetic iron nanoparticles and graphene oxide were synthesized and used as nanosupports for the covalent immobilization of β-glucosidase. This study revealed that the immobilization efficiency depends on the structure and the surface chemistry of nanostructures employed. The hybrid nanostructure-based biocatalysts formed exhibited a two to four-fold higher thermostability as compared to the free enzyme, as well as an enhanced performance at higher temperatures (up to 70°C) and in a wider pH range. Moreover, these biocatalysts retained a significant part of their bioactivity (up to 40%) after 12 repeated reaction cycles.http://journal.frontiersin.org/article/10.3389/fmats.2018.00025/fullbioconjugateimmobilizationgraphenemagnetic nanoparticlesnanoscaffold |
| spellingShingle | Georgios Orfanakis Michaela Patila Alexandra V. Catzikonstantinou Kyriaki-Marina Lyra Antonios Kouloumpis Konstantinos Spyrou Petros Katapodis Alkiviadis Paipetis Petra Rudolf Dimitrios Gournis Haralambos Stamatis Hybrid Nanomaterials of Magnetic Iron Nanoparticles and Graphene Oxide as Matrices for the Immobilization of β-Glucosidase: Synthesis, Characterization, and Biocatalytic Properties Frontiers in Materials bioconjugate immobilization graphene magnetic nanoparticles nanoscaffold |
| title | Hybrid Nanomaterials of Magnetic Iron Nanoparticles and Graphene Oxide as Matrices for the Immobilization of β-Glucosidase: Synthesis, Characterization, and Biocatalytic Properties |
| title_full | Hybrid Nanomaterials of Magnetic Iron Nanoparticles and Graphene Oxide as Matrices for the Immobilization of β-Glucosidase: Synthesis, Characterization, and Biocatalytic Properties |
| title_fullStr | Hybrid Nanomaterials of Magnetic Iron Nanoparticles and Graphene Oxide as Matrices for the Immobilization of β-Glucosidase: Synthesis, Characterization, and Biocatalytic Properties |
| title_full_unstemmed | Hybrid Nanomaterials of Magnetic Iron Nanoparticles and Graphene Oxide as Matrices for the Immobilization of β-Glucosidase: Synthesis, Characterization, and Biocatalytic Properties |
| title_short | Hybrid Nanomaterials of Magnetic Iron Nanoparticles and Graphene Oxide as Matrices for the Immobilization of β-Glucosidase: Synthesis, Characterization, and Biocatalytic Properties |
| title_sort | hybrid nanomaterials of magnetic iron nanoparticles and graphene oxide as matrices for the immobilization of β glucosidase synthesis characterization and biocatalytic properties |
| topic | bioconjugate immobilization graphene magnetic nanoparticles nanoscaffold |
| url | http://journal.frontiersin.org/article/10.3389/fmats.2018.00025/full |
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