A rapid method for DNA Isolation from blood, dried blood spots and rapid diagnosis test
Background & objectives: Malaria is a parasitic disease spread by Plasmodium parasite. Microscopy, lateral flow devices such as the Rapid Diagnostic Test (RDT), molecular methods such as Polymerase Chain Reaction (PCR), isothermal methods such as Loop-mediated isothermal amplification (LAMP), an...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Journal of Vector Borne Diseases |
| Subjects: | |
| Online Access: | http://www.jvbd.org/article.asp?issn=0972-9062;year=2023;volume=60;issue=4;spage=449;epage=452;aulast=Sharma |
| _version_ | 1827300208118071296 |
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| author | Supriya Sharma Aparajita Sen Naseem Ahmed Deendayal Swarnkar Himmat Singh |
| author_facet | Supriya Sharma Aparajita Sen Naseem Ahmed Deendayal Swarnkar Himmat Singh |
| author_sort | Supriya Sharma |
| collection | DOAJ |
| description | Background & objectives: Malaria is a parasitic disease spread by Plasmodium parasite. Microscopy, lateral flow devices such as the Rapid Diagnostic Test (RDT), molecular methods such as Polymerase Chain Reaction (PCR), isothermal methods such as Loop-mediated isothermal amplification (LAMP), and other diagnostic methods are available for malaria. On the other hand, the accuracy of molecular diagnosis is dependent on genomic DNA isolation. A quick method for isolating and subjectively determining the presence of genomic DNA from blood, dried blood spot (DBS), and rapid diagnostic test (RDT), was identified.
Methods: We have developed a protocol for isolating DNA from blood, DBS, and RDTs using the HUDSON Buffer (TCEP and EDTA). Isolated genomic DNA was seen with SYBR Safe DNA stain (1X) under a UV transilluminator without running in 0.8 percent gel electrophoresis or using a spectrophotometer.
Results: The technique for DNA isolation was accurate for the presence of malaria parasite genomic DNA from positive samples confirmed by microscopy with a sensitivity of 76% and specificity of 78.67% and RDT with a sensitivity of 88% and specificity of 66%. The requirements were minimal, and the process took 30 minutes for a hundred sample processing.
Interpretation & conclusion: Finding a fast and reliable method of separating nucleic acids from many samples is crucial. This approach extracts intact genomic DNA in under ten minutes, making it ideal for large-scale investigations. |
| first_indexed | 2024-04-24T15:49:20Z |
| format | Article |
| id | doaj.art-856e3d24b417400e998be19dc01fa0bd |
| institution | Directory Open Access Journal |
| issn | 0972-9062 |
| language | English |
| last_indexed | 2024-04-24T15:49:20Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Vector Borne Diseases |
| spelling | doaj.art-856e3d24b417400e998be19dc01fa0bd2024-04-01T12:56:03ZengWolters Kluwer Medknow PublicationsJournal of Vector Borne Diseases0972-90622023-01-0160444945210.4103/0972-9062.383646A rapid method for DNA Isolation from blood, dried blood spots and rapid diagnosis testSupriya SharmaAparajita SenNaseem AhmedDeendayal SwarnkarHimmat SinghBackground & objectives: Malaria is a parasitic disease spread by Plasmodium parasite. Microscopy, lateral flow devices such as the Rapid Diagnostic Test (RDT), molecular methods such as Polymerase Chain Reaction (PCR), isothermal methods such as Loop-mediated isothermal amplification (LAMP), and other diagnostic methods are available for malaria. On the other hand, the accuracy of molecular diagnosis is dependent on genomic DNA isolation. A quick method for isolating and subjectively determining the presence of genomic DNA from blood, dried blood spot (DBS), and rapid diagnostic test (RDT), was identified. Methods: We have developed a protocol for isolating DNA from blood, DBS, and RDTs using the HUDSON Buffer (TCEP and EDTA). Isolated genomic DNA was seen with SYBR Safe DNA stain (1X) under a UV transilluminator without running in 0.8 percent gel electrophoresis or using a spectrophotometer. Results: The technique for DNA isolation was accurate for the presence of malaria parasite genomic DNA from positive samples confirmed by microscopy with a sensitivity of 76% and specificity of 78.67% and RDT with a sensitivity of 88% and specificity of 66%. The requirements were minimal, and the process took 30 minutes for a hundred sample processing. Interpretation & conclusion: Finding a fast and reliable method of separating nucleic acids from many samples is crucial. This approach extracts intact genomic DNA in under ten minutes, making it ideal for large-scale investigations.http://www.jvbd.org/article.asp?issn=0972-9062;year=2023;volume=60;issue=4;spage=449;epage=452;aulast=Sharmarapid; dna isolation; blood; dried blood spot (dbs); rapid diagnostic kits (rdt) |
| spellingShingle | Supriya Sharma Aparajita Sen Naseem Ahmed Deendayal Swarnkar Himmat Singh A rapid method for DNA Isolation from blood, dried blood spots and rapid diagnosis test Journal of Vector Borne Diseases rapid; dna isolation; blood; dried blood spot (dbs); rapid diagnostic kits (rdt) |
| title | A rapid method for DNA Isolation from blood, dried blood spots and rapid diagnosis test |
| title_full | A rapid method for DNA Isolation from blood, dried blood spots and rapid diagnosis test |
| title_fullStr | A rapid method for DNA Isolation from blood, dried blood spots and rapid diagnosis test |
| title_full_unstemmed | A rapid method for DNA Isolation from blood, dried blood spots and rapid diagnosis test |
| title_short | A rapid method for DNA Isolation from blood, dried blood spots and rapid diagnosis test |
| title_sort | rapid method for dna isolation from blood dried blood spots and rapid diagnosis test |
| topic | rapid; dna isolation; blood; dried blood spot (dbs); rapid diagnostic kits (rdt) |
| url | http://www.jvbd.org/article.asp?issn=0972-9062;year=2023;volume=60;issue=4;spage=449;epage=452;aulast=Sharma |
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