Immunoassay standardization for the detection of immunoglobulin A (IgA) against Porphyromonas gingivalis antigens in saliva of individuals with and without leprosy
Abstract Leprosy reactions are immune processes that cause neural damage in individuals with leprosy. As periodontitis is an infectious disease related to its development, specific antibodies to periodontal pathogens must be evaluated to better understand the humoral mechanisms underlying this relat...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2021-11-01
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| Series: | AMB Express |
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| Online Access: | https://doi.org/10.1186/s13568-021-01312-7 |
| _version_ | 1818927880422293504 |
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| author | Mariana Costa Calheira Soraya Castro Trindade Michelle Miranda Lopes Falcão Luciana Sales Conceição Barbosa Gislene Regina Batista Carvalho Paulo Roberto Lima Machado Isaac Suzart Gomes-Filho Elisangela de Jesus Campos Paulo Cirino de Carvalho-Filho Márcia Tosta Xavier Antonio Pedro Fróes de Farias José Tadeu Raynal Rocha Filho Johelle de Santana Passos-Soares |
| author_facet | Mariana Costa Calheira Soraya Castro Trindade Michelle Miranda Lopes Falcão Luciana Sales Conceição Barbosa Gislene Regina Batista Carvalho Paulo Roberto Lima Machado Isaac Suzart Gomes-Filho Elisangela de Jesus Campos Paulo Cirino de Carvalho-Filho Márcia Tosta Xavier Antonio Pedro Fróes de Farias José Tadeu Raynal Rocha Filho Johelle de Santana Passos-Soares |
| author_sort | Mariana Costa Calheira |
| collection | DOAJ |
| description | Abstract Leprosy reactions are immune processes that cause neural damage in individuals with leprosy. As periodontitis is an infectious disease related to its development, specific antibodies to periodontal pathogens must be evaluated to better understand the humoral mechanisms underlying this relationship. Therefore, the objective of this study was to standardize an immunoassay to measure IgA specific to P. gingivalis antigens in the saliva of individuals with leprosy. An ELISA checkerboard titration was performed. A validation test involving 53 individuals with leprosy, 24 with and 19 without periodontitis, was conducted and a ROC curve constructed to calculate sensitivity and specificity. The coefficient of the optical densities was 2.21 and 2.66 for P. gingivalis crude extract and the recombinant protein HmuY, respectively. Sensitivity and specificity for the P. gingivalis crude extract were 66.7% and 73.7%, respectively, and for HmuY, were 62.5% and 52.6%, respectively. Specific recognition of P. gingivalis occurred predominantly in individuals with periodontitis, which validates the use of this test for studying periodontitis in individuals with leprosy. Trial registration CAEE 64476117.3.0000.0049, 21/07/2017, retrospectively registered |
| first_indexed | 2024-12-20T03:20:02Z |
| format | Article |
| id | doaj.art-85711476866b4d71b73f5978c9051736 |
| institution | Directory Open Access Journal |
| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-20T03:20:02Z |
| publishDate | 2021-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj.art-85711476866b4d71b73f5978c90517362022-12-21T19:55:15ZengSpringerOpenAMB Express2191-08552021-11-011111710.1186/s13568-021-01312-7Immunoassay standardization for the detection of immunoglobulin A (IgA) against Porphyromonas gingivalis antigens in saliva of individuals with and without leprosyMariana Costa Calheira0Soraya Castro Trindade1Michelle Miranda Lopes Falcão2Luciana Sales Conceição Barbosa3Gislene Regina Batista Carvalho4Paulo Roberto Lima Machado5Isaac Suzart Gomes-Filho6Elisangela de Jesus Campos7Paulo Cirino de Carvalho-Filho8Márcia Tosta Xavier9Antonio Pedro Fróes de Farias10José Tadeu Raynal Rocha Filho11Johelle de Santana Passos-Soares12Immunology Department, Federal University of BahiaImmunology Department, Federal University of BahiaImmunology Department, Federal University of BahiaImmunology Department, Federal University of BahiaImmunology Department, Federal University of BahiaImmunology Service, Professor Edgar Santos University Hospital, Federal University of BahiaHealth Department, Feira de Santana State UniversityOral Biochemistry Laboratory, Health Sciences Institute, Federal University of BahiaDental School, Bahiana School of Medicine and Public HealthDental School, Bahiana School of Medicine and Public HealthImmunology and Molecular Biology Laboratory, Federal University of BahiaImmunology and Molecular Biology Laboratory, Federal University of BahiaImmunology Department, Federal University of BahiaAbstract Leprosy reactions are immune processes that cause neural damage in individuals with leprosy. As periodontitis is an infectious disease related to its development, specific antibodies to periodontal pathogens must be evaluated to better understand the humoral mechanisms underlying this relationship. Therefore, the objective of this study was to standardize an immunoassay to measure IgA specific to P. gingivalis antigens in the saliva of individuals with leprosy. An ELISA checkerboard titration was performed. A validation test involving 53 individuals with leprosy, 24 with and 19 without periodontitis, was conducted and a ROC curve constructed to calculate sensitivity and specificity. The coefficient of the optical densities was 2.21 and 2.66 for P. gingivalis crude extract and the recombinant protein HmuY, respectively. Sensitivity and specificity for the P. gingivalis crude extract were 66.7% and 73.7%, respectively, and for HmuY, were 62.5% and 52.6%, respectively. Specific recognition of P. gingivalis occurred predominantly in individuals with periodontitis, which validates the use of this test for studying periodontitis in individuals with leprosy. Trial registration CAEE 64476117.3.0000.0049, 21/07/2017, retrospectively registeredhttps://doi.org/10.1186/s13568-021-01312-7LeprosyPeriodontitisSalivaImmunoassay protocolsEnzyme-linked immunosorbent assayP. gingivalis |
| spellingShingle | Mariana Costa Calheira Soraya Castro Trindade Michelle Miranda Lopes Falcão Luciana Sales Conceição Barbosa Gislene Regina Batista Carvalho Paulo Roberto Lima Machado Isaac Suzart Gomes-Filho Elisangela de Jesus Campos Paulo Cirino de Carvalho-Filho Márcia Tosta Xavier Antonio Pedro Fróes de Farias José Tadeu Raynal Rocha Filho Johelle de Santana Passos-Soares Immunoassay standardization for the detection of immunoglobulin A (IgA) against Porphyromonas gingivalis antigens in saliva of individuals with and without leprosy AMB Express Leprosy Periodontitis Saliva Immunoassay protocols Enzyme-linked immunosorbent assay P. gingivalis |
| title | Immunoassay standardization for the detection of immunoglobulin A (IgA) against Porphyromonas gingivalis antigens in saliva of individuals with and without leprosy |
| title_full | Immunoassay standardization for the detection of immunoglobulin A (IgA) against Porphyromonas gingivalis antigens in saliva of individuals with and without leprosy |
| title_fullStr | Immunoassay standardization for the detection of immunoglobulin A (IgA) against Porphyromonas gingivalis antigens in saliva of individuals with and without leprosy |
| title_full_unstemmed | Immunoassay standardization for the detection of immunoglobulin A (IgA) against Porphyromonas gingivalis antigens in saliva of individuals with and without leprosy |
| title_short | Immunoassay standardization for the detection of immunoglobulin A (IgA) against Porphyromonas gingivalis antigens in saliva of individuals with and without leprosy |
| title_sort | immunoassay standardization for the detection of immunoglobulin a iga against porphyromonas gingivalis antigens in saliva of individuals with and without leprosy |
| topic | Leprosy Periodontitis Saliva Immunoassay protocols Enzyme-linked immunosorbent assay P. gingivalis |
| url | https://doi.org/10.1186/s13568-021-01312-7 |
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