Vestibular Compensation after Vestibular Dysfunction Induced by Arsanilic Acid in Mice
When vestibular function is lost, vestibular compensation works for the reacquisition of body balance. For the study of vestibular dysfunction and vestibular compensation, surgical or chemical labyrinthectomy has been performed in various animal species. In the present study, we performed chemical l...
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MDPI AG
2019-11-01
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| Series: | Brain Sciences |
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| Online Access: | https://www.mdpi.com/2076-3425/9/11/329 |
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| author | Taeko Ito Kouko Tatsumi Yasumitsu Takimoto Tadashi Nishimura Takao Imai Toshiaki Yamanaka Noriaki Takeda Akio Wanaka Tadashi Kitahara |
| author_facet | Taeko Ito Kouko Tatsumi Yasumitsu Takimoto Tadashi Nishimura Takao Imai Toshiaki Yamanaka Noriaki Takeda Akio Wanaka Tadashi Kitahara |
| author_sort | Taeko Ito |
| collection | DOAJ |
| description | When vestibular function is lost, vestibular compensation works for the reacquisition of body balance. For the study of vestibular dysfunction and vestibular compensation, surgical or chemical labyrinthectomy has been performed in various animal species. In the present study, we performed chemical labyrinthectomy using arsanilic acid in mice and investigated the time course of vestibular compensation through behavioral observations and histological studies. The surgical procedures required only paracentesis and storage of 50 µL of <i>p</i>-arsanilic acid sodium salt solution in the tympanic cavity for 5 min. From behavioral observations, vestibular functions were worst at 2 days and recovered by 7 days after surgery. Spontaneous nystagmus appeared at 1 day after surgery with arsanilic acid and disappeared by 2 days. Histological studies revealed specific damage to the vestibular endorgans. In the ipsilateral spinal vestibular nucleus, the medial vestibular nucleus, and the contralateral prepositus hypoglossal nucleus, a substantial number of c-Fos-immunoreactive cells appeared by 1 day after surgery with arsanilic acid, with a maximum increase in number by 2 days and complete disappearance by 7 days. Taken together, these findings indicate that chemical labyrinthectomy with arsanilic acid and the subsequent observation of vestibular compensation is a useful strategy for elucidation of the molecular mechanisms underlying vestibular pathophysiologies. |
| first_indexed | 2024-04-12T04:12:07Z |
| format | Article |
| id | doaj.art-85749d64f65a410b831e4e81bb50b515 |
| institution | Directory Open Access Journal |
| issn | 2076-3425 |
| language | English |
| last_indexed | 2024-04-12T04:12:07Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
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| series | Brain Sciences |
| spelling | doaj.art-85749d64f65a410b831e4e81bb50b5152022-12-22T03:48:29ZengMDPI AGBrain Sciences2076-34252019-11-0191132910.3390/brainsci9110329brainsci9110329Vestibular Compensation after Vestibular Dysfunction Induced by Arsanilic Acid in MiceTaeko Ito0Kouko Tatsumi1Yasumitsu Takimoto2Tadashi Nishimura3Takao Imai4Toshiaki Yamanaka5Noriaki Takeda6Akio Wanaka7Tadashi Kitahara8Department of Otolaryngology-Head and Neck Surgery, Nara Medical University, Kashihara 634-8522, JapanDepartment of Anatomy and Neuroscience, Nara Medical University, Kashihara 634-8521, JapanDepartment of Otolaryngology-Head and Neck Surgery, Osaka University Graduate School of Medicine, Suita 565-0871, JapanDepartment of Otolaryngology-Head and Neck Surgery, Nara Medical University, Kashihara 634-8522, JapanDepartment of Otolaryngology-Head and Neck Surgery, Osaka University Graduate School of Medicine, Suita 565-0871, JapanDepartment of Otolaryngology-Head and Neck Surgery, Nara Medical University, Kashihara 634-8522, JapanDepartment of Otolaryngology, University of Tokushima School of Medicine, Tokushima 770-8503, JapanDepartment of Anatomy and Neuroscience, Nara Medical University, Kashihara 634-8521, JapanDepartment of Otolaryngology-Head and Neck Surgery, Nara Medical University, Kashihara 634-8522, JapanWhen vestibular function is lost, vestibular compensation works for the reacquisition of body balance. For the study of vestibular dysfunction and vestibular compensation, surgical or chemical labyrinthectomy has been performed in various animal species. In the present study, we performed chemical labyrinthectomy using arsanilic acid in mice and investigated the time course of vestibular compensation through behavioral observations and histological studies. The surgical procedures required only paracentesis and storage of 50 µL of <i>p</i>-arsanilic acid sodium salt solution in the tympanic cavity for 5 min. From behavioral observations, vestibular functions were worst at 2 days and recovered by 7 days after surgery. Spontaneous nystagmus appeared at 1 day after surgery with arsanilic acid and disappeared by 2 days. Histological studies revealed specific damage to the vestibular endorgans. In the ipsilateral spinal vestibular nucleus, the medial vestibular nucleus, and the contralateral prepositus hypoglossal nucleus, a substantial number of c-Fos-immunoreactive cells appeared by 1 day after surgery with arsanilic acid, with a maximum increase in number by 2 days and complete disappearance by 7 days. Taken together, these findings indicate that chemical labyrinthectomy with arsanilic acid and the subsequent observation of vestibular compensation is a useful strategy for elucidation of the molecular mechanisms underlying vestibular pathophysiologies.https://www.mdpi.com/2076-3425/9/11/329mouselabyrinthectomy<i>p</i>-arsanilic acidc-fosarczif268vestibular nucleushead deviationnystagmusvestibular compensation |
| spellingShingle | Taeko Ito Kouko Tatsumi Yasumitsu Takimoto Tadashi Nishimura Takao Imai Toshiaki Yamanaka Noriaki Takeda Akio Wanaka Tadashi Kitahara Vestibular Compensation after Vestibular Dysfunction Induced by Arsanilic Acid in Mice Brain Sciences mouse labyrinthectomy <i>p</i>-arsanilic acid c-fos arc zif268 vestibular nucleus head deviation nystagmus vestibular compensation |
| title | Vestibular Compensation after Vestibular Dysfunction Induced by Arsanilic Acid in Mice |
| title_full | Vestibular Compensation after Vestibular Dysfunction Induced by Arsanilic Acid in Mice |
| title_fullStr | Vestibular Compensation after Vestibular Dysfunction Induced by Arsanilic Acid in Mice |
| title_full_unstemmed | Vestibular Compensation after Vestibular Dysfunction Induced by Arsanilic Acid in Mice |
| title_short | Vestibular Compensation after Vestibular Dysfunction Induced by Arsanilic Acid in Mice |
| title_sort | vestibular compensation after vestibular dysfunction induced by arsanilic acid in mice |
| topic | mouse labyrinthectomy <i>p</i>-arsanilic acid c-fos arc zif268 vestibular nucleus head deviation nystagmus vestibular compensation |
| url | https://www.mdpi.com/2076-3425/9/11/329 |
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