Microcrater-Arrayed Chemiluminescence Cell Chip to Boost Anti-Cancer Drug Administration in Zebrafish Tumor Xenograft Model
Purpose: The aim of this study was to develop a rapid and automatic drug screening platform using microcrater-arrayed (µCA) cell chips. Methods: The µCA chip was fabricated using a laser direct writing technique. The fabrication time required for one 9 × 9 microarray wax chip was as quick as 1 min....
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/11/1/4 |
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| author | Ching-Te Kuo Yu-Sheng Lai Siang-Rong Lu Hsinyu Lee Hsiu-Hao Chang |
| author_facet | Ching-Te Kuo Yu-Sheng Lai Siang-Rong Lu Hsinyu Lee Hsiu-Hao Chang |
| author_sort | Ching-Te Kuo |
| collection | DOAJ |
| description | Purpose: The aim of this study was to develop a rapid and automatic drug screening platform using microcrater-arrayed (µCA) cell chips. Methods: The µCA chip was fabricated using a laser direct writing technique. The fabrication time required for one 9 × 9 microarray wax chip was as quick as 1 min. On a nanodroplet handling platform, the chip was pre-coated with anti-cancer drugs, including cyclophosphamide, cisplatin, doxorubicin, oncovin, etoposide, and 5-fluorouracil, and their associated mixtures. Cell droplets containing 100 SK-N-DZ or MCF-7 cells were then loaded onto the chip. Cell viability was examined directly through a chemiluminescence assay on the chip using the CellTiter-Glo assay. Results: The time needed for the drug screening assay was demonstrated to be less than 30 s for a total of 81 tests. The prediction of optimal drug synergy from the µCA chip was found by matching it to that of the zebrafish MCF-7 tumor xenograft model, instead of the conventional 96-well plate assay. In addition, the critical reagent volume and cell number for each µCA chip test were 200 nL and 100 cells, respectively, which were significantly lower than 100 µL and 4000 cells, which were achieved using the 96-well assay. Conclusion: Our study for the µCA chip platform could improve the high-throughput drug synergy screening targeting the applications of tumor cell biology. |
| first_indexed | 2024-03-10T01:54:04Z |
| format | Article |
| id | doaj.art-8576c4f28e6e46b7a57f01704fbd3a4d |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T01:54:04Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-8576c4f28e6e46b7a57f01704fbd3a4d2023-11-23T12:59:33ZengMDPI AGBiology2079-77372021-12-01111410.3390/biology11010004Microcrater-Arrayed Chemiluminescence Cell Chip to Boost Anti-Cancer Drug Administration in Zebrafish Tumor Xenograft ModelChing-Te Kuo0Yu-Sheng Lai1Siang-Rong Lu2Hsinyu Lee3Hsiu-Hao Chang4Department of Mechanical and Electro-Mechanical Engineering, National Sun Yat-sen University, Kaohsiung 80424, TaiwanDepartment of Life Science, National Taiwan University, Taipei 10617, TaiwanDepartment of Life Science, National Taiwan University, Taipei 10617, TaiwanDepartment of Life Science, National Taiwan University, Taipei 10617, TaiwanDepartment of Pediatrics, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei 10617, TaiwanPurpose: The aim of this study was to develop a rapid and automatic drug screening platform using microcrater-arrayed (µCA) cell chips. Methods: The µCA chip was fabricated using a laser direct writing technique. The fabrication time required for one 9 × 9 microarray wax chip was as quick as 1 min. On a nanodroplet handling platform, the chip was pre-coated with anti-cancer drugs, including cyclophosphamide, cisplatin, doxorubicin, oncovin, etoposide, and 5-fluorouracil, and their associated mixtures. Cell droplets containing 100 SK-N-DZ or MCF-7 cells were then loaded onto the chip. Cell viability was examined directly through a chemiluminescence assay on the chip using the CellTiter-Glo assay. Results: The time needed for the drug screening assay was demonstrated to be less than 30 s for a total of 81 tests. The prediction of optimal drug synergy from the µCA chip was found by matching it to that of the zebrafish MCF-7 tumor xenograft model, instead of the conventional 96-well plate assay. In addition, the critical reagent volume and cell number for each µCA chip test were 200 nL and 100 cells, respectively, which were significantly lower than 100 µL and 4000 cells, which were achieved using the 96-well assay. Conclusion: Our study for the µCA chip platform could improve the high-throughput drug synergy screening targeting the applications of tumor cell biology.https://www.mdpi.com/2079-7737/11/1/4drug screeningzebrafish xenograftmicroarray chipchemiluminescence |
| spellingShingle | Ching-Te Kuo Yu-Sheng Lai Siang-Rong Lu Hsinyu Lee Hsiu-Hao Chang Microcrater-Arrayed Chemiluminescence Cell Chip to Boost Anti-Cancer Drug Administration in Zebrafish Tumor Xenograft Model Biology drug screening zebrafish xenograft microarray chip chemiluminescence |
| title | Microcrater-Arrayed Chemiluminescence Cell Chip to Boost Anti-Cancer Drug Administration in Zebrafish Tumor Xenograft Model |
| title_full | Microcrater-Arrayed Chemiluminescence Cell Chip to Boost Anti-Cancer Drug Administration in Zebrafish Tumor Xenograft Model |
| title_fullStr | Microcrater-Arrayed Chemiluminescence Cell Chip to Boost Anti-Cancer Drug Administration in Zebrafish Tumor Xenograft Model |
| title_full_unstemmed | Microcrater-Arrayed Chemiluminescence Cell Chip to Boost Anti-Cancer Drug Administration in Zebrafish Tumor Xenograft Model |
| title_short | Microcrater-Arrayed Chemiluminescence Cell Chip to Boost Anti-Cancer Drug Administration in Zebrafish Tumor Xenograft Model |
| title_sort | microcrater arrayed chemiluminescence cell chip to boost anti cancer drug administration in zebrafish tumor xenograft model |
| topic | drug screening zebrafish xenograft microarray chip chemiluminescence |
| url | https://www.mdpi.com/2079-7737/11/1/4 |
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