Simple procedure for assessing diffuse subarachnoid hemorrhage successfully created using filament perforation method in mice
Abstract The murine model of subarachnoid hemorrhage (SAH) is a valuable experimental tool for investigating molecular and cellular mechanisms, and the endovascular filament perforation technique can be used to simulate prominent pathophysiological features observed after human SAH; however, current...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-02-01
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| Series: | Animal Models and Experimental Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ame2.12372 |
| _version_ | 1797246460863971328 |
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| author | Tatsushi Mutoh Ryota Tochinai Hiroaki Aono Masayoshi Kuwahara Yasuyuki Taki Tatsuya Ishikawa |
| author_facet | Tatsushi Mutoh Ryota Tochinai Hiroaki Aono Masayoshi Kuwahara Yasuyuki Taki Tatsuya Ishikawa |
| author_sort | Tatsushi Mutoh |
| collection | DOAJ |
| description | Abstract The murine model of subarachnoid hemorrhage (SAH) is a valuable experimental tool for investigating molecular and cellular mechanisms, and the endovascular filament perforation technique can be used to simulate prominent pathophysiological features observed after human SAH; however, current validation methods for assessing an appropriate SAH model are limited. Here, we introduce a simple procedure for selecting a mouse model of diffuse SAH. SAH was induced in 24 mice using a standard filament perforation method. After confirming survival at 24 h, SAH was scored 0–1 based on T2*‐weighted images on whole‐brain magnetic resonance imaging (MRI) and visual surveillance of the cisterna magna (CM) through the dura mater. The CM‐based SAH grading correlated well with a reference parameter defined by extracted brain (r2 = 0.53, p < 0.0001). The receiver operating characteristic curve revealed a sensitivity of 85% and a specificity of 91% for detecting diffuse SAH, with a similar area under the curve (0.89 ± 0.06 [standard error of the mean]) as the MRI‐based grading (0.72 ± 0.10, p = 0.12). Our data suggest that confirming an SAH clot in the CM is a valuable way to select a clinically relevant diffuse SAH model that can be used in future experimental studies. |
| first_indexed | 2024-04-24T19:43:09Z |
| format | Article |
| id | doaj.art-857a464e6a194a0b8235b3509412e693 |
| institution | Directory Open Access Journal |
| issn | 2576-2095 |
| language | English |
| last_indexed | 2024-04-24T19:43:09Z |
| publishDate | 2024-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Animal Models and Experimental Medicine |
| spelling | doaj.art-857a464e6a194a0b8235b3509412e6932024-03-25T10:18:10ZengWileyAnimal Models and Experimental Medicine2576-20952024-02-0171778110.1002/ame2.12372Simple procedure for assessing diffuse subarachnoid hemorrhage successfully created using filament perforation method in miceTatsushi Mutoh0Ryota Tochinai1Hiroaki Aono2Masayoshi Kuwahara3Yasuyuki Taki4Tatsuya Ishikawa5Department of Surgical Neurology, Research Institute for Brain and Blood Vessels Akita Cerebrospinal and Cardiovascular Center Akita JapanDepartment of Surgical Neurology, Research Institute for Brain and Blood Vessels Akita Cerebrospinal and Cardiovascular Center Akita JapanDepartment of Surgical Neurology, Research Institute for Brain and Blood Vessels Akita Cerebrospinal and Cardiovascular Center Akita JapanDepartment of Veterinary Pathophysiology and Animal Health, Graduate School of Agriculture and Life Sciences The University of Tokyo Tokyo JapanDepartment of Aging Research and Geriatric Medicine, Institute of Development, Aging and Cancer Tohoku University Sendai JapanDepartment of Surgical Neurology, Research Institute for Brain and Blood Vessels Akita Cerebrospinal and Cardiovascular Center Akita JapanAbstract The murine model of subarachnoid hemorrhage (SAH) is a valuable experimental tool for investigating molecular and cellular mechanisms, and the endovascular filament perforation technique can be used to simulate prominent pathophysiological features observed after human SAH; however, current validation methods for assessing an appropriate SAH model are limited. Here, we introduce a simple procedure for selecting a mouse model of diffuse SAH. SAH was induced in 24 mice using a standard filament perforation method. After confirming survival at 24 h, SAH was scored 0–1 based on T2*‐weighted images on whole‐brain magnetic resonance imaging (MRI) and visual surveillance of the cisterna magna (CM) through the dura mater. The CM‐based SAH grading correlated well with a reference parameter defined by extracted brain (r2 = 0.53, p < 0.0001). The receiver operating characteristic curve revealed a sensitivity of 85% and a specificity of 91% for detecting diffuse SAH, with a similar area under the curve (0.89 ± 0.06 [standard error of the mean]) as the MRI‐based grading (0.72 ± 0.10, p = 0.12). Our data suggest that confirming an SAH clot in the CM is a valuable way to select a clinically relevant diffuse SAH model that can be used in future experimental studies.https://doi.org/10.1002/ame2.12372cisterna magnaclot distributionfilament perforationmouse modelsubarachnoid hemorrhage |
| spellingShingle | Tatsushi Mutoh Ryota Tochinai Hiroaki Aono Masayoshi Kuwahara Yasuyuki Taki Tatsuya Ishikawa Simple procedure for assessing diffuse subarachnoid hemorrhage successfully created using filament perforation method in mice Animal Models and Experimental Medicine cisterna magna clot distribution filament perforation mouse model subarachnoid hemorrhage |
| title | Simple procedure for assessing diffuse subarachnoid hemorrhage successfully created using filament perforation method in mice |
| title_full | Simple procedure for assessing diffuse subarachnoid hemorrhage successfully created using filament perforation method in mice |
| title_fullStr | Simple procedure for assessing diffuse subarachnoid hemorrhage successfully created using filament perforation method in mice |
| title_full_unstemmed | Simple procedure for assessing diffuse subarachnoid hemorrhage successfully created using filament perforation method in mice |
| title_short | Simple procedure for assessing diffuse subarachnoid hemorrhage successfully created using filament perforation method in mice |
| title_sort | simple procedure for assessing diffuse subarachnoid hemorrhage successfully created using filament perforation method in mice |
| topic | cisterna magna clot distribution filament perforation mouse model subarachnoid hemorrhage |
| url | https://doi.org/10.1002/ame2.12372 |
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