Perception of Biocontrol Potential of <i>Bacillus inaquosorum</i> KR2-7 against Tomato Fusarium Wilt through Merging Genome Mining with Chemical Analysis
Tomato Fusarium wilt, caused by <i>Fusarium oxysporum</i> f. sp. <i>lycopersici</i> (<i>Fol</i>), is a destructive disease that threatens the agricultural production of tomatoes. In the present study, the biocontrol potential of strain KR2-7 against <i>Fol&l...
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MDPI AG
2022-01-01
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| author | Maedeh Kamali Dianjing Guo Shahram Naeimi Jafar Ahmadi |
| author_facet | Maedeh Kamali Dianjing Guo Shahram Naeimi Jafar Ahmadi |
| author_sort | Maedeh Kamali |
| collection | DOAJ |
| description | Tomato Fusarium wilt, caused by <i>Fusarium oxysporum</i> f. sp. <i>lycopersici</i> (<i>Fol</i>), is a destructive disease that threatens the agricultural production of tomatoes. In the present study, the biocontrol potential of strain KR2-7 against <i>Fol</i> was investigated through integrated genome mining and chemical analysis. Strain KR2-7 was identified as <i>B. inaquosorum</i> based on phylogenetic analysis. Through the genome mining of strain KR2-7, we identified nine antifungal and antibacterial compound biosynthetic gene clusters (BGCs) including fengycin, surfactin and Bacillomycin F, bacillaene, macrolactin, sporulation killing factor (skf), subtilosin A, bacilysin, and bacillibactin. The corresponding compounds were confirmed through MALDI-TOF-MS chemical analysis. The gene/gene clusters involved in plant colonization, plant growth promotion, and induced systemic resistance were also identified in the KR2-7 genome, and their related secondary metabolites were detected. In light of these results, the biocontrol potential of strain KR2-7 against tomato Fusarium wilt was identified. This study highlights the potential to use strain KR2-7 as a plant-growth promotion agent. |
| first_indexed | 2024-03-10T01:53:02Z |
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| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T01:53:02Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-857c035543824b229a2bb81755eb10e32023-11-23T13:01:51ZengMDPI AGBiology2079-77372022-01-0111113710.3390/biology11010137Perception of Biocontrol Potential of <i>Bacillus inaquosorum</i> KR2-7 against Tomato Fusarium Wilt through Merging Genome Mining with Chemical AnalysisMaedeh Kamali0Dianjing Guo1Shahram Naeimi2Jafar Ahmadi3College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Hong Kong, ChinaState Key Laboratory of Agrobiotechnology and School of Life Sciences, Chinese University of Hong Kong, Hong Kong, ChinaDepartment of Biological Control Research, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran 19858-13111, IranDepartment of Genetics and Plant Breeding, Imam Khomeini International University, Qazvin 34149-16818, IranTomato Fusarium wilt, caused by <i>Fusarium oxysporum</i> f. sp. <i>lycopersici</i> (<i>Fol</i>), is a destructive disease that threatens the agricultural production of tomatoes. In the present study, the biocontrol potential of strain KR2-7 against <i>Fol</i> was investigated through integrated genome mining and chemical analysis. Strain KR2-7 was identified as <i>B. inaquosorum</i> based on phylogenetic analysis. Through the genome mining of strain KR2-7, we identified nine antifungal and antibacterial compound biosynthetic gene clusters (BGCs) including fengycin, surfactin and Bacillomycin F, bacillaene, macrolactin, sporulation killing factor (skf), subtilosin A, bacilysin, and bacillibactin. The corresponding compounds were confirmed through MALDI-TOF-MS chemical analysis. The gene/gene clusters involved in plant colonization, plant growth promotion, and induced systemic resistance were also identified in the KR2-7 genome, and their related secondary metabolites were detected. In light of these results, the biocontrol potential of strain KR2-7 against tomato Fusarium wilt was identified. This study highlights the potential to use strain KR2-7 as a plant-growth promotion agent.https://www.mdpi.com/2079-7737/11/1/137Fusarium wiltbiocontrol<i>B. inaquosorum</i> KR2-7genome mininggene clustersMALDI-TOF-MS |
| spellingShingle | Maedeh Kamali Dianjing Guo Shahram Naeimi Jafar Ahmadi Perception of Biocontrol Potential of <i>Bacillus inaquosorum</i> KR2-7 against Tomato Fusarium Wilt through Merging Genome Mining with Chemical Analysis Biology Fusarium wilt biocontrol <i>B. inaquosorum</i> KR2-7 genome mining gene clusters MALDI-TOF-MS |
| title | Perception of Biocontrol Potential of <i>Bacillus inaquosorum</i> KR2-7 against Tomato Fusarium Wilt through Merging Genome Mining with Chemical Analysis |
| title_full | Perception of Biocontrol Potential of <i>Bacillus inaquosorum</i> KR2-7 against Tomato Fusarium Wilt through Merging Genome Mining with Chemical Analysis |
| title_fullStr | Perception of Biocontrol Potential of <i>Bacillus inaquosorum</i> KR2-7 against Tomato Fusarium Wilt through Merging Genome Mining with Chemical Analysis |
| title_full_unstemmed | Perception of Biocontrol Potential of <i>Bacillus inaquosorum</i> KR2-7 against Tomato Fusarium Wilt through Merging Genome Mining with Chemical Analysis |
| title_short | Perception of Biocontrol Potential of <i>Bacillus inaquosorum</i> KR2-7 against Tomato Fusarium Wilt through Merging Genome Mining with Chemical Analysis |
| title_sort | perception of biocontrol potential of i bacillus inaquosorum i kr2 7 against tomato fusarium wilt through merging genome mining with chemical analysis |
| topic | Fusarium wilt biocontrol <i>B. inaquosorum</i> KR2-7 genome mining gene clusters MALDI-TOF-MS |
| url | https://www.mdpi.com/2079-7737/11/1/137 |
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