Metabolic Engineering Strategies for Improved Lipid Production and Cellular Physiological Responses in Yeast <i>Saccharomyces cerevisiae</i>
Microbial lipids have been a hot topic in the field of metabolic engineering and synthetic biology due to their increased market and important applications in biofuels, oleochemicals, cosmetics, etc. This review first compares the popular hosts for lipid production and explains the four modules for...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/5/427 |
| _version_ | 1797498742409003008 |
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| author | Wei Jiang Chao Li Yanjun Li Huadong Peng |
| author_facet | Wei Jiang Chao Li Yanjun Li Huadong Peng |
| author_sort | Wei Jiang |
| collection | DOAJ |
| description | Microbial lipids have been a hot topic in the field of metabolic engineering and synthetic biology due to their increased market and important applications in biofuels, oleochemicals, cosmetics, etc. This review first compares the popular hosts for lipid production and explains the four modules for lipid synthesis in yeast, including the fatty acid biosynthesis module, lipid accumulation module, lipid sequestration module, and fatty acid modification module. This is followed by a summary of metabolic engineering strategies that could be used for enhancing each module for lipid production. In addition, the efforts being invested in improving the production of value-added fatty acids in engineered yeast, such as cyclopropane fatty acid, ricinoleic acid, gamma linoleic acid, EPA, and DHA, are included. A discussion is further made on the potential relationships between lipid pathway engineering and consequential changes in cellular physiological properties, such as cell membrane integrity, intracellular reactive oxygen species level, and mitochondrial membrane potential. Finally, with the rapid development of synthetic biology tools, such as CRISPR genome editing tools and machine learning models, this review proposes some future trends that could be employed to engineer yeast with enhanced intracellular lipid production while not compromising much of its cellular health. |
| first_indexed | 2024-03-10T03:37:42Z |
| format | Article |
| id | doaj.art-8580a0a8d6324be5a3fcad5bfdf76e00 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T03:37:42Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
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| series | Journal of Fungi |
| spelling | doaj.art-8580a0a8d6324be5a3fcad5bfdf76e002023-11-23T11:40:54ZengMDPI AGJournal of Fungi2309-608X2022-04-018542710.3390/jof8050427Metabolic Engineering Strategies for Improved Lipid Production and Cellular Physiological Responses in Yeast <i>Saccharomyces cerevisiae</i>Wei Jiang0Chao Li1Yanjun Li2Huadong Peng3Department of Chemical and Biological Engineering, Monash University, Clayton, VIC 3800, AustraliaState Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200234, ChinaCollege of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, ChinaDepartment of Chemical and Biological Engineering, Monash University, Clayton, VIC 3800, AustraliaMicrobial lipids have been a hot topic in the field of metabolic engineering and synthetic biology due to their increased market and important applications in biofuels, oleochemicals, cosmetics, etc. This review first compares the popular hosts for lipid production and explains the four modules for lipid synthesis in yeast, including the fatty acid biosynthesis module, lipid accumulation module, lipid sequestration module, and fatty acid modification module. This is followed by a summary of metabolic engineering strategies that could be used for enhancing each module for lipid production. In addition, the efforts being invested in improving the production of value-added fatty acids in engineered yeast, such as cyclopropane fatty acid, ricinoleic acid, gamma linoleic acid, EPA, and DHA, are included. A discussion is further made on the potential relationships between lipid pathway engineering and consequential changes in cellular physiological properties, such as cell membrane integrity, intracellular reactive oxygen species level, and mitochondrial membrane potential. Finally, with the rapid development of synthetic biology tools, such as CRISPR genome editing tools and machine learning models, this review proposes some future trends that could be employed to engineer yeast with enhanced intracellular lipid production while not compromising much of its cellular health.https://www.mdpi.com/2309-608X/8/5/427metabolic engineeringsynthetic biologyyeasttriacylglycerolcellular physiologyfatty acid |
| spellingShingle | Wei Jiang Chao Li Yanjun Li Huadong Peng Metabolic Engineering Strategies for Improved Lipid Production and Cellular Physiological Responses in Yeast <i>Saccharomyces cerevisiae</i> Journal of Fungi metabolic engineering synthetic biology yeast triacylglycerol cellular physiology fatty acid |
| title | Metabolic Engineering Strategies for Improved Lipid Production and Cellular Physiological Responses in Yeast <i>Saccharomyces cerevisiae</i> |
| title_full | Metabolic Engineering Strategies for Improved Lipid Production and Cellular Physiological Responses in Yeast <i>Saccharomyces cerevisiae</i> |
| title_fullStr | Metabolic Engineering Strategies for Improved Lipid Production and Cellular Physiological Responses in Yeast <i>Saccharomyces cerevisiae</i> |
| title_full_unstemmed | Metabolic Engineering Strategies for Improved Lipid Production and Cellular Physiological Responses in Yeast <i>Saccharomyces cerevisiae</i> |
| title_short | Metabolic Engineering Strategies for Improved Lipid Production and Cellular Physiological Responses in Yeast <i>Saccharomyces cerevisiae</i> |
| title_sort | metabolic engineering strategies for improved lipid production and cellular physiological responses in yeast i saccharomyces cerevisiae i |
| topic | metabolic engineering synthetic biology yeast triacylglycerol cellular physiology fatty acid |
| url | https://www.mdpi.com/2309-608X/8/5/427 |
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