Association between leptin gene polymorphism and growth traits in Bali cattle
Leptin (LEP) gene produces hormone leptin which is secreted by adipose tissue, and plays an im-portant role in energy balance, regulating feed intake, regulating endocrine function, and immune functions. This study aims to identify molecular markers of LEP gene and its association with growth traits...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Diponegoro University, Faculty of Animal and Agricultural Sciences
2023-03-01
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| Series: | Journal of the Indonesian Tropical Animal Agriculture |
| Subjects: | |
| Online Access: | https://ejournal.undip.ac.id/index.php/jitaa/article/view/46386 |
| _version_ | 1797868083724943360 |
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| author | T. Kurlyana T. Hartatik S. Sumadi |
| author_facet | T. Kurlyana T. Hartatik S. Sumadi |
| author_sort | T. Kurlyana |
| collection | DOAJ |
| description | Leptin (LEP) gene produces hormone leptin which is secreted by adipose tissue, and plays an im-portant role in energy balance, regulating feed intake, regulating endocrine function, and immune functions. This study aims to identify molecular markers of LEP gene and its association with growth traits based on SNP in Bali cattle. The blood samples were collected from 16 male and 30 female Bali cattle. The growth data were recorded from 2018 to 2020, consist of body weight and body size. Am-plification of leptin gene with polymerase chain reaction (PCR) using pair of primers, Lep-tin_3Forward: 5’- AGCTTGGAAACATGGTGGTC-3’ and Leptin_3Reverse: 5’- CATGATGCTCCCTGGATTCT-3’ with DNA target 898 bp. The SNPs were identified by the direct sequencing technique. Genotypes of the SNPs were identified using sequencing method. Association of LEP genotypes with growth traits was performed using oneway ANOVA. Three DNA polymor-phisms of the LEP gene were found, namely g.2913C/T, g.3260T/C, and g.3549G/A. SNP g.2913C/T was significantly associated (P<0,05) with weaning shoulder height (WSH), weaning body length (WBL), weaning chest circumference (WCC), yearling shoulder height (YSH), yearling body length (YBL), and yearling chest circumference (YCC). Meanwhile, SNPs g.3260T/C and g.3549G/A were not associated with the body weight and body size in Bali cattle. In conclusion, the SNP g.2913C/T can be used as molecular marker for body size in weaning and yearling of Bali Cattle. |
| first_indexed | 2024-04-09T23:51:33Z |
| format | Article |
| id | doaj.art-8580f6aa4ca8428e9e7f1bd3b59e3c91 |
| institution | Directory Open Access Journal |
| issn | 2087-8273 2460-6278 |
| language | English |
| last_indexed | 2024-04-09T23:51:33Z |
| publishDate | 2023-03-01 |
| publisher | Diponegoro University, Faculty of Animal and Agricultural Sciences |
| record_format | Article |
| series | Journal of the Indonesian Tropical Animal Agriculture |
| spelling | doaj.art-8580f6aa4ca8428e9e7f1bd3b59e3c912023-03-17T09:05:30ZengDiponegoro University, Faculty of Animal and Agricultural SciencesJournal of the Indonesian Tropical Animal Agriculture2087-82732460-62782023-03-014811910.14710/jitaa.48.1.1-921717Association between leptin gene polymorphism and growth traits in Bali cattleT. Kurlyana0T. Hartatik1S. Sumadi2Graduate Student at Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, IndonesiaDepartment of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, Indonesia2Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Gadjah Mada, IndonesiaLeptin (LEP) gene produces hormone leptin which is secreted by adipose tissue, and plays an im-portant role in energy balance, regulating feed intake, regulating endocrine function, and immune functions. This study aims to identify molecular markers of LEP gene and its association with growth traits based on SNP in Bali cattle. The blood samples were collected from 16 male and 30 female Bali cattle. The growth data were recorded from 2018 to 2020, consist of body weight and body size. Am-plification of leptin gene with polymerase chain reaction (PCR) using pair of primers, Lep-tin_3Forward: 5’- AGCTTGGAAACATGGTGGTC-3’ and Leptin_3Reverse: 5’- CATGATGCTCCCTGGATTCT-3’ with DNA target 898 bp. The SNPs were identified by the direct sequencing technique. Genotypes of the SNPs were identified using sequencing method. Association of LEP genotypes with growth traits was performed using oneway ANOVA. Three DNA polymor-phisms of the LEP gene were found, namely g.2913C/T, g.3260T/C, and g.3549G/A. SNP g.2913C/T was significantly associated (P<0,05) with weaning shoulder height (WSH), weaning body length (WBL), weaning chest circumference (WCC), yearling shoulder height (YSH), yearling body length (YBL), and yearling chest circumference (YCC). Meanwhile, SNPs g.3260T/C and g.3549G/A were not associated with the body weight and body size in Bali cattle. In conclusion, the SNP g.2913C/T can be used as molecular marker for body size in weaning and yearling of Bali Cattle.https://ejournal.undip.ac.id/index.php/jitaa/article/view/46386bali cattleleptin genegrowth traitsmolecular markerssnp |
| spellingShingle | T. Kurlyana T. Hartatik S. Sumadi Association between leptin gene polymorphism and growth traits in Bali cattle Journal of the Indonesian Tropical Animal Agriculture bali cattle leptin gene growth traits molecular markers snp |
| title | Association between leptin gene polymorphism and growth traits in Bali cattle |
| title_full | Association between leptin gene polymorphism and growth traits in Bali cattle |
| title_fullStr | Association between leptin gene polymorphism and growth traits in Bali cattle |
| title_full_unstemmed | Association between leptin gene polymorphism and growth traits in Bali cattle |
| title_short | Association between leptin gene polymorphism and growth traits in Bali cattle |
| title_sort | association between leptin gene polymorphism and growth traits in bali cattle |
| topic | bali cattle leptin gene growth traits molecular markers snp |
| url | https://ejournal.undip.ac.id/index.php/jitaa/article/view/46386 |
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