Development and validation of a method for the quantification of fructooligosaccharides in a prebiotic ice cream
Context: Fructooligosaccharides (FOS) are known as oligofructanes, oligosaccharides or oligofructose, which fall within the concept of prebiotics. One of the methods most commonly used in the industry for quantification and quality control nutraceutical substances classification is the method of hig...
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| Format: | Article |
| Language: | English |
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GarVal Editorial Ltda.
2018-02-01
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| Series: | Journal of Pharmacy & Pharmacognosy Research |
| Subjects: | |
| Online Access: | http://jppres.com/jppres/pdf/vol6/jppres17.302_6.2.108.pdf |
| _version_ | 1818567252726775808 |
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| author | Claudia L. González-Aguirre Juan S. Ramírez-Navas |
| author_facet | Claudia L. González-Aguirre Juan S. Ramírez-Navas |
| author_sort | Claudia L. González-Aguirre |
| collection | DOAJ |
| description | Context: Fructooligosaccharides (FOS) are known as oligofructanes, oligosaccharides or oligofructose, which fall within the concept of prebiotics. One of the methods most commonly used in the industry for quantification and quality control nutraceutical substances classification is the method of high performance liquid chromatography (HPLC).
Aims: To develop a procedure for the determination of FOS by HPLC in raw materials and a prebiotic ice cream.
Methods: For the chromatographic separation, an HPLC was used with a refractive index detector (IR). The separation was performed using two columns coupled Sugar-pak I™ using an isocratic procedure with water type 1 at 0.35 mL/min. Kestose (GF2), nistose (GF3) and fructofuranosylnystose (GF4) were used as standards. Robustness was assessed by applying the Youden and Steiner test.
Results: Good linear correlations were obtained (y = 14191.4470 x + 285684.2, r2 = 0.9904) within the concentration range of 8.0-12.0 mg/mL. The FOS recoveries were 99.5% with the intra-day and inter-day relative standard deviation (RSD) less than 0.8%. The robustness test showed that the temperature parameters of the column and flow velocity are critical factors in the method.
Conclusions: This reliable, simple and cost-effective method could be applied to the routine monitoring of FOS (GF2, GF3, and GF4) in raw materials and prebiotic ice creams. |
| first_indexed | 2024-12-14T06:21:03Z |
| format | Article |
| id | doaj.art-8582aa19115140afa498f557569d2416 |
| institution | Directory Open Access Journal |
| issn | 0719-4250 |
| language | English |
| last_indexed | 2024-12-14T06:21:03Z |
| publishDate | 2018-02-01 |
| publisher | GarVal Editorial Ltda. |
| record_format | Article |
| series | Journal of Pharmacy & Pharmacognosy Research |
| spelling | doaj.art-8582aa19115140afa498f557569d24162022-12-21T23:13:50ZengGarVal Editorial Ltda.Journal of Pharmacy & Pharmacognosy Research0719-42502018-02-0162108116Development and validation of a method for the quantification of fructooligosaccharides in a prebiotic ice creamClaudia L. González-Aguirre0Juan S. Ramírez-Navas1Grupo GIEMA, Programa de Química, Universidad Santiago de Cali, Campus Pampalinda, Calle 5 con Carrera 62. Santiago de Cali, Colombia.Grupo GIEMA, Programa de Química, Universidad Santiago de Cali, Campus Pampalinda, Calle 5 con Carrera 62. Santiago de Cali, Colombia. Grupo GIPAB, Escuela de Ingeniería de Alimentos, Universidad del Valle. Ciudad Universitaria Meléndez, Calle 13 # 100-00, Espacio 338. Santiago de Cali, Colombia.Context: Fructooligosaccharides (FOS) are known as oligofructanes, oligosaccharides or oligofructose, which fall within the concept of prebiotics. One of the methods most commonly used in the industry for quantification and quality control nutraceutical substances classification is the method of high performance liquid chromatography (HPLC). Aims: To develop a procedure for the determination of FOS by HPLC in raw materials and a prebiotic ice cream. Methods: For the chromatographic separation, an HPLC was used with a refractive index detector (IR). The separation was performed using two columns coupled Sugar-pak I™ using an isocratic procedure with water type 1 at 0.35 mL/min. Kestose (GF2), nistose (GF3) and fructofuranosylnystose (GF4) were used as standards. Robustness was assessed by applying the Youden and Steiner test. Results: Good linear correlations were obtained (y = 14191.4470 x + 285684.2, r2 = 0.9904) within the concentration range of 8.0-12.0 mg/mL. The FOS recoveries were 99.5% with the intra-day and inter-day relative standard deviation (RSD) less than 0.8%. The robustness test showed that the temperature parameters of the column and flow velocity are critical factors in the method. Conclusions: This reliable, simple and cost-effective method could be applied to the routine monitoring of FOS (GF2, GF3, and GF4) in raw materials and prebiotic ice creams.http://jppres.com/jppres/pdf/vol6/jppres17.302_6.2.108.pdffructofuranosylnystoseice creamkestosenystoseprebioticrefractive index |
| spellingShingle | Claudia L. González-Aguirre Juan S. Ramírez-Navas Development and validation of a method for the quantification of fructooligosaccharides in a prebiotic ice cream Journal of Pharmacy & Pharmacognosy Research fructofuranosylnystose ice cream kestose nystose prebiotic refractive index |
| title | Development and validation of a method for the quantification of fructooligosaccharides in a prebiotic ice cream |
| title_full | Development and validation of a method for the quantification of fructooligosaccharides in a prebiotic ice cream |
| title_fullStr | Development and validation of a method for the quantification of fructooligosaccharides in a prebiotic ice cream |
| title_full_unstemmed | Development and validation of a method for the quantification of fructooligosaccharides in a prebiotic ice cream |
| title_short | Development and validation of a method for the quantification of fructooligosaccharides in a prebiotic ice cream |
| title_sort | development and validation of a method for the quantification of fructooligosaccharides in a prebiotic ice cream |
| topic | fructofuranosylnystose ice cream kestose nystose prebiotic refractive index |
| url | http://jppres.com/jppres/pdf/vol6/jppres17.302_6.2.108.pdf |
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