Polydopamine Linking Substrate for AMPs: Characterisation and Stability on Ti6Al4V
Infections are common complications in joint replacement surgeries. Eradicated infections can lead to implant failure. In this paper, analogues of the peptide KR-12 derived from the human cathelicidin LL-37 were designed, synthesised, and characterised. The designed antimicrobial peptides (AMPs) wer...
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MDPI AG
2020-08-01
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| Series: | Materials |
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| Online Access: | https://www.mdpi.com/1996-1944/13/17/3714 |
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| author | Zuzanna Trzcińska Marc Bruggeman Hanieh Ijakipour Nikolas J. Hodges James Bowen Artemis Stamboulis |
| author_facet | Zuzanna Trzcińska Marc Bruggeman Hanieh Ijakipour Nikolas J. Hodges James Bowen Artemis Stamboulis |
| author_sort | Zuzanna Trzcińska |
| collection | DOAJ |
| description | Infections are common complications in joint replacement surgeries. Eradicated infections can lead to implant failure. In this paper, analogues of the peptide KR-12 derived from the human cathelicidin LL-37 were designed, synthesised, and characterised. The designed antimicrobial peptides (AMPs) were attached to the surface of a titanium alloy, Ti6Al4V, by conjugation to a polydopamine linking substrate. The topography of the polydopamine coating was evaluated by electron microscopy and coating thickness measurements were performed with ellipsometry and Atomic Force Microscopy (AFM). The subsequently attached peptide stability was investigated with release profile studies in simulated body fluid, using both fluorescence imaging and High-Performance Liquid Chromatography (HPLC). Finally, the hydrophobicity of the coating was characterised by water contact angle measurements. The designed AMPs were shown to provide long-term bonding to the polydopamine-coated Ti6Al4V surfaces. |
| first_indexed | 2024-03-10T16:59:03Z |
| format | Article |
| id | doaj.art-8583907bba3c42bf999125aaa36d1f37 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-10T16:59:03Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-8583907bba3c42bf999125aaa36d1f372023-11-20T11:01:25ZengMDPI AGMaterials1996-19442020-08-011317371410.3390/ma13173714Polydopamine Linking Substrate for AMPs: Characterisation and Stability on Ti6Al4VZuzanna Trzcińska0Marc Bruggeman1Hanieh Ijakipour2Nikolas J. Hodges3James Bowen4Artemis Stamboulis5School of Metallurgy and Materials, University of Birmingham, Edgbaston, Birmingham B15 2TT, UKSchool of Metallurgy and Materials, University of Birmingham, Edgbaston, Birmingham B15 2TT, UKSchool of Metallurgy and Materials, University of Birmingham, Edgbaston, Birmingham B15 2TT, UKSchool of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UKSchool of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham B15 2TT, UKSchool of Metallurgy and Materials, University of Birmingham, Edgbaston, Birmingham B15 2TT, UKInfections are common complications in joint replacement surgeries. Eradicated infections can lead to implant failure. In this paper, analogues of the peptide KR-12 derived from the human cathelicidin LL-37 were designed, synthesised, and characterised. The designed antimicrobial peptides (AMPs) were attached to the surface of a titanium alloy, Ti6Al4V, by conjugation to a polydopamine linking substrate. The topography of the polydopamine coating was evaluated by electron microscopy and coating thickness measurements were performed with ellipsometry and Atomic Force Microscopy (AFM). The subsequently attached peptide stability was investigated with release profile studies in simulated body fluid, using both fluorescence imaging and High-Performance Liquid Chromatography (HPLC). Finally, the hydrophobicity of the coating was characterised by water contact angle measurements. The designed AMPs were shown to provide long-term bonding to the polydopamine-coated Ti6Al4V surfaces.https://www.mdpi.com/1996-1944/13/17/3714Ti6Al4Vpolydopamineantimicrobial peptidescathelicidinKR-12 |
| spellingShingle | Zuzanna Trzcińska Marc Bruggeman Hanieh Ijakipour Nikolas J. Hodges James Bowen Artemis Stamboulis Polydopamine Linking Substrate for AMPs: Characterisation and Stability on Ti6Al4V Materials Ti6Al4V polydopamine antimicrobial peptides cathelicidin KR-12 |
| title | Polydopamine Linking Substrate for AMPs: Characterisation and Stability on Ti6Al4V |
| title_full | Polydopamine Linking Substrate for AMPs: Characterisation and Stability on Ti6Al4V |
| title_fullStr | Polydopamine Linking Substrate for AMPs: Characterisation and Stability on Ti6Al4V |
| title_full_unstemmed | Polydopamine Linking Substrate for AMPs: Characterisation and Stability on Ti6Al4V |
| title_short | Polydopamine Linking Substrate for AMPs: Characterisation and Stability on Ti6Al4V |
| title_sort | polydopamine linking substrate for amps characterisation and stability on ti6al4v |
| topic | Ti6Al4V polydopamine antimicrobial peptides cathelicidin KR-12 |
| url | https://www.mdpi.com/1996-1944/13/17/3714 |
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