The Biocontrol of Plant Pathogenic Fungi by Selected Lactic Acid Bacteria: From Laboratory to Field Study
Plant diseases caused by pathogenic fungi generate large losses in crops and pose a threat to human and animal health. Since the European Green Deal put a strong emphasis on the need to reduce the use of chemical plant protection, interest in biological control has been growing. The present study ai...
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| Format: | Article |
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MDPI AG
2023-12-01
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| Series: | Agriculture |
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| Online Access: | https://www.mdpi.com/2077-0472/14/1/61 |
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| author | Romuald Gwiazdowski Krzysztof Kubiak Krzysztof Juś Katarzyna Marchwińska Daniela Gwiazdowska |
| author_facet | Romuald Gwiazdowski Krzysztof Kubiak Krzysztof Juś Katarzyna Marchwińska Daniela Gwiazdowska |
| author_sort | Romuald Gwiazdowski |
| collection | DOAJ |
| description | Plant diseases caused by pathogenic fungi generate large losses in crops and pose a threat to human and animal health. Since the European Green Deal put a strong emphasis on the need to reduce the use of chemical plant protection, interest in biological control has been growing. The present study aimed to investigate the efficacy of lactic acid bacteria (LAB) isolated from silages in the control of pathogenic fungi through in vitro, mini-plot, and field experiments. The tested LAB showed antifungal activity in vitro towards strains from the <i>Fusarium</i>, <i>Alternaria</i>, <i>Rhizoctonia</i>, <i>Colletotrichum,</i> and <i>Sclerotinia</i> genera; however, only five strains reached an activity ≥ 400 AU/mL towards all pathogenic fungi. The selected strains demonstrated high efficacy in reducing disease symptoms in plants in the mini-plot and field experiments. In the mini-plot experiment, stem smut of rye and wheat common bunt were reduced in the range 34.5–94.7% and 24.8–99.6%, respectively. In the field experiments, the efficacy of LAB in the control of rye and wheat disease differed and reached over 90% in some trials. The effectiveness of LAB in the control of seedling blight did not exceed 70%. A significant increase in yield (from 42.86 to 195.65%) was observed mainly in wheat cultivation. The increase in rye yield was observed only in chosen trials. No phytotoxicity was observed. The results indicate the potential possibilities of using LAB as a biocontrol agent. |
| first_indexed | 2024-03-08T09:59:56Z |
| format | Article |
| id | doaj.art-858bb183b2464864909288a2c19982fb |
| institution | Directory Open Access Journal |
| issn | 2077-0472 |
| language | English |
| last_indexed | 2024-03-08T09:59:56Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agriculture |
| spelling | doaj.art-858bb183b2464864909288a2c19982fb2024-01-29T13:39:30ZengMDPI AGAgriculture2077-04722023-12-011416110.3390/agriculture14010061The Biocontrol of Plant Pathogenic Fungi by Selected Lactic Acid Bacteria: From Laboratory to Field StudyRomuald Gwiazdowski0Krzysztof Kubiak1Krzysztof Juś2Katarzyna Marchwińska3Daniela Gwiazdowska4Research Centre for Registration of Agrochemicals, Institute of Plant Protection—National Research Institute, Władysława Węgorka 20, 60-318 Poznań, PolandResearch Centre for Registration of Agrochemicals, Institute of Plant Protection—National Research Institute, Władysława Węgorka 20, 60-318 Poznań, PolandDepartment of Natural Science and Quality Assurance, Institute of Quality Science, Poznań University of Economics and Business, Niepodległości 10, 61-875 Poznań, PolandDepartment of Natural Science and Quality Assurance, Institute of Quality Science, Poznań University of Economics and Business, Niepodległości 10, 61-875 Poznań, PolandDepartment of Natural Science and Quality Assurance, Institute of Quality Science, Poznań University of Economics and Business, Niepodległości 10, 61-875 Poznań, PolandPlant diseases caused by pathogenic fungi generate large losses in crops and pose a threat to human and animal health. Since the European Green Deal put a strong emphasis on the need to reduce the use of chemical plant protection, interest in biological control has been growing. The present study aimed to investigate the efficacy of lactic acid bacteria (LAB) isolated from silages in the control of pathogenic fungi through in vitro, mini-plot, and field experiments. The tested LAB showed antifungal activity in vitro towards strains from the <i>Fusarium</i>, <i>Alternaria</i>, <i>Rhizoctonia</i>, <i>Colletotrichum,</i> and <i>Sclerotinia</i> genera; however, only five strains reached an activity ≥ 400 AU/mL towards all pathogenic fungi. The selected strains demonstrated high efficacy in reducing disease symptoms in plants in the mini-plot and field experiments. In the mini-plot experiment, stem smut of rye and wheat common bunt were reduced in the range 34.5–94.7% and 24.8–99.6%, respectively. In the field experiments, the efficacy of LAB in the control of rye and wheat disease differed and reached over 90% in some trials. The effectiveness of LAB in the control of seedling blight did not exceed 70%. A significant increase in yield (from 42.86 to 195.65%) was observed mainly in wheat cultivation. The increase in rye yield was observed only in chosen trials. No phytotoxicity was observed. The results indicate the potential possibilities of using LAB as a biocontrol agent.https://www.mdpi.com/2077-0472/14/1/61biological agentsbiological controlintegrated pest managementlactic acid bacteriasustainable agriculture |
| spellingShingle | Romuald Gwiazdowski Krzysztof Kubiak Krzysztof Juś Katarzyna Marchwińska Daniela Gwiazdowska The Biocontrol of Plant Pathogenic Fungi by Selected Lactic Acid Bacteria: From Laboratory to Field Study Agriculture biological agents biological control integrated pest management lactic acid bacteria sustainable agriculture |
| title | The Biocontrol of Plant Pathogenic Fungi by Selected Lactic Acid Bacteria: From Laboratory to Field Study |
| title_full | The Biocontrol of Plant Pathogenic Fungi by Selected Lactic Acid Bacteria: From Laboratory to Field Study |
| title_fullStr | The Biocontrol of Plant Pathogenic Fungi by Selected Lactic Acid Bacteria: From Laboratory to Field Study |
| title_full_unstemmed | The Biocontrol of Plant Pathogenic Fungi by Selected Lactic Acid Bacteria: From Laboratory to Field Study |
| title_short | The Biocontrol of Plant Pathogenic Fungi by Selected Lactic Acid Bacteria: From Laboratory to Field Study |
| title_sort | biocontrol of plant pathogenic fungi by selected lactic acid bacteria from laboratory to field study |
| topic | biological agents biological control integrated pest management lactic acid bacteria sustainable agriculture |
| url | https://www.mdpi.com/2077-0472/14/1/61 |
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