Gubenyiliu II Inhibits Breast Tumor Growth and Metastasis Associated with Decreased Heparanase Expression and Phosphorylation of ERK and AKT Pathways
Gubenyiliu II (GYII), a Traditional Chinese Medicine (TCM) formula used in our hospital, has shown beneficial effects in cancer patients. In this study, we investigated the molecular mechanisms underlying the beneficial effects of GYII on murine breast cancer models. GYII showed significant inhibito...
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MDPI AG
2017-05-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/22/5/787 |
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| author | Yi Zhang Gan-Lin Zhang Xu Sun Ke-Xin Cao Ya-Wen Shang Mu-Xin Gong Cong Ma Nan Nan Jin-Ping Li Ming-Wei Yu Guo-Wang Yang Xiao-Min Wang |
| author_facet | Yi Zhang Gan-Lin Zhang Xu Sun Ke-Xin Cao Ya-Wen Shang Mu-Xin Gong Cong Ma Nan Nan Jin-Ping Li Ming-Wei Yu Guo-Wang Yang Xiao-Min Wang |
| author_sort | Yi Zhang |
| collection | DOAJ |
| description | Gubenyiliu II (GYII), a Traditional Chinese Medicine (TCM) formula used in our hospital, has shown beneficial effects in cancer patients. In this study, we investigated the molecular mechanisms underlying the beneficial effects of GYII on murine breast cancer models. GYII showed significant inhibitory effects on tumor growth and metastasis in the murine breast cancer model. Additionally, GYII suppressed the proliferation of 4T1 and MCF-7 cells in a dose-dependent manner. A better inhibitory effect on 4T1 cell proliferation and migration was found in the decomposed recipes (DR) of GYII. Moreover, heparanase expression and the degree of angiogenesis were reduced in tumor tissues. Western blot analysis showed decreased expression of heparanase and growth factors in the cells treated with GYII and its decomposed recipes (DR2 and DR3), and thereby a reduction in the phosphorylation of extracellular signal-regulated kinase (ERK) and serine-threonine kinase (AKT). These results suggest that GYII exerts anti-tumor growth and anti-metastatic effects in the murine breast cancer model. The anti-tumor activity of GYII and its decomposed recipes is, at least partly, associated with decreased heparanase and growth factor expression, which subsequently suppressed the activation of the ERK and AKT pathways. |
| first_indexed | 2024-04-12T13:11:53Z |
| format | Article |
| id | doaj.art-858eea67a41846ec89097bce49688461 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-12T13:11:53Z |
| publishDate | 2017-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-858eea67a41846ec89097bce496884612022-12-22T03:31:49ZengMDPI AGMolecules1420-30492017-05-0122578710.3390/molecules22050787molecules22050787Gubenyiliu II Inhibits Breast Tumor Growth and Metastasis Associated with Decreased Heparanase Expression and Phosphorylation of ERK and AKT PathwaysYi Zhang0Gan-Lin Zhang1Xu Sun2Ke-Xin Cao3Ya-Wen Shang4Mu-Xin Gong5Cong Ma6Nan Nan7Jin-Ping Li8Ming-Wei Yu9Guo-Wang Yang10Xiao-Min Wang11Department of Oncology, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, ChinaDepartment of Oncology, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, ChinaDepartment of Oncology, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, ChinaDepartment of Oncology, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, ChinaSchool of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, ChinaSchool of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, ChinaDepartment of Oncology, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, ChinaDepartment of Oncology, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, ChinaDepartment of Oncology, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, ChinaDepartment of Oncology, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, ChinaDepartment of Oncology, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, ChinaDepartment of Oncology, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, ChinaGubenyiliu II (GYII), a Traditional Chinese Medicine (TCM) formula used in our hospital, has shown beneficial effects in cancer patients. In this study, we investigated the molecular mechanisms underlying the beneficial effects of GYII on murine breast cancer models. GYII showed significant inhibitory effects on tumor growth and metastasis in the murine breast cancer model. Additionally, GYII suppressed the proliferation of 4T1 and MCF-7 cells in a dose-dependent manner. A better inhibitory effect on 4T1 cell proliferation and migration was found in the decomposed recipes (DR) of GYII. Moreover, heparanase expression and the degree of angiogenesis were reduced in tumor tissues. Western blot analysis showed decreased expression of heparanase and growth factors in the cells treated with GYII and its decomposed recipes (DR2 and DR3), and thereby a reduction in the phosphorylation of extracellular signal-regulated kinase (ERK) and serine-threonine kinase (AKT). These results suggest that GYII exerts anti-tumor growth and anti-metastatic effects in the murine breast cancer model. The anti-tumor activity of GYII and its decomposed recipes is, at least partly, associated with decreased heparanase and growth factor expression, which subsequently suppressed the activation of the ERK and AKT pathways.http://www.mdpi.com/1420-3049/22/5/787Gubenyiliu IIbreast tumorheparanasegrowth factorsERK and AKT pathways |
| spellingShingle | Yi Zhang Gan-Lin Zhang Xu Sun Ke-Xin Cao Ya-Wen Shang Mu-Xin Gong Cong Ma Nan Nan Jin-Ping Li Ming-Wei Yu Guo-Wang Yang Xiao-Min Wang Gubenyiliu II Inhibits Breast Tumor Growth and Metastasis Associated with Decreased Heparanase Expression and Phosphorylation of ERK and AKT Pathways Molecules Gubenyiliu II breast tumor heparanase growth factors ERK and AKT pathways |
| title | Gubenyiliu II Inhibits Breast Tumor Growth and Metastasis Associated with Decreased Heparanase Expression and Phosphorylation of ERK and AKT Pathways |
| title_full | Gubenyiliu II Inhibits Breast Tumor Growth and Metastasis Associated with Decreased Heparanase Expression and Phosphorylation of ERK and AKT Pathways |
| title_fullStr | Gubenyiliu II Inhibits Breast Tumor Growth and Metastasis Associated with Decreased Heparanase Expression and Phosphorylation of ERK and AKT Pathways |
| title_full_unstemmed | Gubenyiliu II Inhibits Breast Tumor Growth and Metastasis Associated with Decreased Heparanase Expression and Phosphorylation of ERK and AKT Pathways |
| title_short | Gubenyiliu II Inhibits Breast Tumor Growth and Metastasis Associated with Decreased Heparanase Expression and Phosphorylation of ERK and AKT Pathways |
| title_sort | gubenyiliu ii inhibits breast tumor growth and metastasis associated with decreased heparanase expression and phosphorylation of erk and akt pathways |
| topic | Gubenyiliu II breast tumor heparanase growth factors ERK and AKT pathways |
| url | http://www.mdpi.com/1420-3049/22/5/787 |
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