Genome-wide survey of sucrose non-fermenting 1-related protein kinase 2 in Rosaceae and expression analysis of PbrSnRK2 in response to ABA stress
Abstract Background The members of the sucrose non-fermenting 1-related protein kinase 2 (SnRK2) family are specific serine/threonine protein kinases in plants that play important roles in stress signal transduction and adaptation. Because of their positive regulatory roles in response to adverse co...
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| Format: | Article |
| Language: | English |
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BMC
2020-11-01
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| Series: | BMC Genomics |
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| Online Access: | http://link.springer.com/article/10.1186/s12864-020-07201-w |
| _version_ | 1819161742502002688 |
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| author | Guodong Chen Jizhong Wang Xin Qiao Cong Jin Weike Duan Xiaochuan Sun Juyou Wu |
| author_facet | Guodong Chen Jizhong Wang Xin Qiao Cong Jin Weike Duan Xiaochuan Sun Juyou Wu |
| author_sort | Guodong Chen |
| collection | DOAJ |
| description | Abstract Background The members of the sucrose non-fermenting 1-related protein kinase 2 (SnRK2) family are specific serine/threonine protein kinases in plants that play important roles in stress signal transduction and adaptation. Because of their positive regulatory roles in response to adverse conditions, the genes encoding thes proteins are considered potential candidates for breeding of plants for disease resistance and genetic improvement. However, there is far less information about this kinase family, and the function of these genes has not been explored in Rosaceae. Results A genome-wide survey and analysis of the genes encoding members of the SnRK2 family were performed in pear (Pyrus bretschneideri) and seven other Rosaceae species. A total of 71 SnRK2 genes were identified from the eight Rosaceae species and classified into three subgroups based on phylogenetic analysis and structural characteristics. Purifying selection played a crucial role in the evolution of SnRK2 genes, and whole-genome duplication and dispersed duplication were the primary forces underlying the characteristics of the SnRK2 gene family in Rosaceae. Transcriptome data and qRT-PCR assay results revealed that the distribution of PbrSnRK2s was very extensive, including across the roots, leaves, pollen, styles, and flowers, although most of them were mainly expressed in leaves. In addition, under stress conditions, the transcript levels of some of the genes were upregulated in leaves in response to ABA treatment. Conclusions This study provides useful information and a theoretical introduction for the study of the evolution, expression, and functions of the SnRK2 gene family in plants. |
| first_indexed | 2024-12-22T17:17:11Z |
| format | Article |
| id | doaj.art-85911052f94c43cf88def72e2c9b53b3 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-22T17:17:11Z |
| publishDate | 2020-11-01 |
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| series | BMC Genomics |
| spelling | doaj.art-85911052f94c43cf88def72e2c9b53b32022-12-21T18:18:55ZengBMCBMC Genomics1471-21642020-11-0121111710.1186/s12864-020-07201-wGenome-wide survey of sucrose non-fermenting 1-related protein kinase 2 in Rosaceae and expression analysis of PbrSnRK2 in response to ABA stressGuodong Chen0Jizhong Wang1Xin Qiao2Cong Jin3Weike Duan4Xiaochuan Sun5Juyou Wu6College of Life Science and Food Engineering, Huaiyin Institute of TechnologyCollege of Life Science and Food Engineering, Huaiyin Institute of TechnologyCenter of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural UniversityCollege of Life Science and Food Engineering, Huaiyin Institute of TechnologyCollege of Life Science and Food Engineering, Huaiyin Institute of TechnologyCollege of Life Science and Food Engineering, Huaiyin Institute of TechnologyCenter of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural UniversityAbstract Background The members of the sucrose non-fermenting 1-related protein kinase 2 (SnRK2) family are specific serine/threonine protein kinases in plants that play important roles in stress signal transduction and adaptation. Because of their positive regulatory roles in response to adverse conditions, the genes encoding thes proteins are considered potential candidates for breeding of plants for disease resistance and genetic improvement. However, there is far less information about this kinase family, and the function of these genes has not been explored in Rosaceae. Results A genome-wide survey and analysis of the genes encoding members of the SnRK2 family were performed in pear (Pyrus bretschneideri) and seven other Rosaceae species. A total of 71 SnRK2 genes were identified from the eight Rosaceae species and classified into three subgroups based on phylogenetic analysis and structural characteristics. Purifying selection played a crucial role in the evolution of SnRK2 genes, and whole-genome duplication and dispersed duplication were the primary forces underlying the characteristics of the SnRK2 gene family in Rosaceae. Transcriptome data and qRT-PCR assay results revealed that the distribution of PbrSnRK2s was very extensive, including across the roots, leaves, pollen, styles, and flowers, although most of them were mainly expressed in leaves. In addition, under stress conditions, the transcript levels of some of the genes were upregulated in leaves in response to ABA treatment. Conclusions This study provides useful information and a theoretical introduction for the study of the evolution, expression, and functions of the SnRK2 gene family in plants.http://link.springer.com/article/10.1186/s12864-020-07201-wSnRK2PearExpression analysisABAAbiotic stress |
| spellingShingle | Guodong Chen Jizhong Wang Xin Qiao Cong Jin Weike Duan Xiaochuan Sun Juyou Wu Genome-wide survey of sucrose non-fermenting 1-related protein kinase 2 in Rosaceae and expression analysis of PbrSnRK2 in response to ABA stress BMC Genomics SnRK2 Pear Expression analysis ABA Abiotic stress |
| title | Genome-wide survey of sucrose non-fermenting 1-related protein kinase 2 in Rosaceae and expression analysis of PbrSnRK2 in response to ABA stress |
| title_full | Genome-wide survey of sucrose non-fermenting 1-related protein kinase 2 in Rosaceae and expression analysis of PbrSnRK2 in response to ABA stress |
| title_fullStr | Genome-wide survey of sucrose non-fermenting 1-related protein kinase 2 in Rosaceae and expression analysis of PbrSnRK2 in response to ABA stress |
| title_full_unstemmed | Genome-wide survey of sucrose non-fermenting 1-related protein kinase 2 in Rosaceae and expression analysis of PbrSnRK2 in response to ABA stress |
| title_short | Genome-wide survey of sucrose non-fermenting 1-related protein kinase 2 in Rosaceae and expression analysis of PbrSnRK2 in response to ABA stress |
| title_sort | genome wide survey of sucrose non fermenting 1 related protein kinase 2 in rosaceae and expression analysis of pbrsnrk2 in response to aba stress |
| topic | SnRK2 Pear Expression analysis ABA Abiotic stress |
| url | http://link.springer.com/article/10.1186/s12864-020-07201-w |
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