In situ activity-based protein profiling of serine hydrolases in E. coli

In situ activity-based protein profiling of serine hydrolases in E. coli

A fluorophosphonate based alkyne activity probe was used for the selective labeling of active serine hydrolases in intact Escherichia coli cells. A biotin-azide tag was subsequently attached to the alkyne functionality of the probe with copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. C...

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Bibliographic Details
Main Authors: Dmitry Shamshurin, Oleg V. Krokhin, David Levin, Richard Sparling, John A. Wilkins
Format: Article
Language:English
Published: Elsevier 2014-09-01
Series:EuPA Open Proteomics
Subjects:
Serine hydrolase
E. coli
ABPP
In situ
Fluorophosphonate probe
Online Access:http://www.sciencedirect.com/science/article/pii/S2212968514000324

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