The hominoid-specific gene TBC1D3 promotes generation of basal neural progenitors and induces cortical folding in mice
Cortical expansion and folding are often linked to the evolution of higher intelligence, but molecular and cellular mechanisms underlying cortical folding remain poorly understood. The hominoid-specific gene TBC1D3 undergoes segmental duplications during hominoid evolution, but its role in brain dev...
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eLife Sciences Publications Ltd
2016-08-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/18197 |
| _version_ | 1811202568983937024 |
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| author | Xiang-Chun Ju Qiong-Qiong Hou Ai-Li Sheng Kong-Yan Wu Yang Zhou Ying Jin Tieqiao Wen Zhengang Yang Xiaoqun Wang Zhen-Ge Luo |
| author_facet | Xiang-Chun Ju Qiong-Qiong Hou Ai-Li Sheng Kong-Yan Wu Yang Zhou Ying Jin Tieqiao Wen Zhengang Yang Xiaoqun Wang Zhen-Ge Luo |
| author_sort | Xiang-Chun Ju |
| collection | DOAJ |
| description | Cortical expansion and folding are often linked to the evolution of higher intelligence, but molecular and cellular mechanisms underlying cortical folding remain poorly understood. The hominoid-specific gene TBC1D3 undergoes segmental duplications during hominoid evolution, but its role in brain development has not been explored. Here, we found that expression of TBC1D3 in ventricular cortical progenitors of mice via in utero electroporation caused delamination of ventricular radial glia cells (vRGs) and promoted generation of self-renewing basal progenitors with typical morphology of outer radial glia (oRG), which are most abundant in primates. Furthermore, down-regulation of TBC1D3 in cultured human brain slices decreased generation of oRGs. Interestingly, localized oRG proliferation resulting from either in utero electroporation or transgenic expression of TBC1D3, was often found to underlie cortical regions exhibiting folding. Thus, we have identified a hominoid gene that is required for oRG generation in regulating the cortical expansion and folding. |
| first_indexed | 2024-04-12T02:40:51Z |
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| id | doaj.art-85954edf19c042f9a1c4a7d4e1509792 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:40:51Z |
| publishDate | 2016-08-01 |
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| record_format | Article |
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| spelling | doaj.art-85954edf19c042f9a1c4a7d4e15097922022-12-22T03:51:18ZengeLife Sciences Publications LtdeLife2050-084X2016-08-01510.7554/eLife.18197The hominoid-specific gene TBC1D3 promotes generation of basal neural progenitors and induces cortical folding in miceXiang-Chun Ju0Qiong-Qiong Hou1Ai-Li Sheng2Kong-Yan Wu3Yang Zhou4Ying Jin5Tieqiao Wen6Zhengang Yang7Xiaoqun Wang8Zhen-Ge Luo9https://orcid.org/0000-0001-5037-0542Institute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China; State Key Laboratory of Neuroscience, Chinese Academy of Sciences, Shanghai, China; Chinese Academy of Sciences University, Beijing, ChinaInstitute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China; State Key Laboratory of Neuroscience, Chinese Academy of Sciences, Shanghai, China; Chinese Academy of Sciences University, Beijing, ChinaInstitute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China; State Key Laboratory of Neuroscience, Chinese Academy of Sciences, Shanghai, ChinaInstitute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China; State Key Laboratory of Neuroscience, Chinese Academy of Sciences, Shanghai, ChinaThe Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaThe Institute of Health Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, ChinaSchool of Life Sciences, Shanghai University, Shanghai, ChinaInstitutes of Brain Science, State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai, ChinaCAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai, China; Institute of Biophysics, Chinese Academy of Sciences, Beijing, ChinaInstitute of Neuroscience, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China; State Key Laboratory of Neuroscience, Chinese Academy of Sciences, Shanghai, China; Chinese Academy of Sciences University, Beijing, China; CAS Center for Excellence in Brain Science and Intelligence Technology, Shanghai, China; ShanghaiTech University, Shanghai, ChinaCortical expansion and folding are often linked to the evolution of higher intelligence, but molecular and cellular mechanisms underlying cortical folding remain poorly understood. The hominoid-specific gene TBC1D3 undergoes segmental duplications during hominoid evolution, but its role in brain development has not been explored. Here, we found that expression of TBC1D3 in ventricular cortical progenitors of mice via in utero electroporation caused delamination of ventricular radial glia cells (vRGs) and promoted generation of self-renewing basal progenitors with typical morphology of outer radial glia (oRG), which are most abundant in primates. Furthermore, down-regulation of TBC1D3 in cultured human brain slices decreased generation of oRGs. Interestingly, localized oRG proliferation resulting from either in utero electroporation or transgenic expression of TBC1D3, was often found to underlie cortical regions exhibiting folding. Thus, we have identified a hominoid gene that is required for oRG generation in regulating the cortical expansion and folding.https://elifesciences.org/articles/18197neural progenitorscortical foldinghominoid-specific gene |
| spellingShingle | Xiang-Chun Ju Qiong-Qiong Hou Ai-Li Sheng Kong-Yan Wu Yang Zhou Ying Jin Tieqiao Wen Zhengang Yang Xiaoqun Wang Zhen-Ge Luo The hominoid-specific gene TBC1D3 promotes generation of basal neural progenitors and induces cortical folding in mice eLife neural progenitors cortical folding hominoid-specific gene |
| title | The hominoid-specific gene TBC1D3 promotes generation of basal neural progenitors and induces cortical folding in mice |
| title_full | The hominoid-specific gene TBC1D3 promotes generation of basal neural progenitors and induces cortical folding in mice |
| title_fullStr | The hominoid-specific gene TBC1D3 promotes generation of basal neural progenitors and induces cortical folding in mice |
| title_full_unstemmed | The hominoid-specific gene TBC1D3 promotes generation of basal neural progenitors and induces cortical folding in mice |
| title_short | The hominoid-specific gene TBC1D3 promotes generation of basal neural progenitors and induces cortical folding in mice |
| title_sort | hominoid specific gene tbc1d3 promotes generation of basal neural progenitors and induces cortical folding in mice |
| topic | neural progenitors cortical folding hominoid-specific gene |
| url | https://elifesciences.org/articles/18197 |
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