Comparative Evaluation of heat-inactivated ABO isoagglutinin titers with solid-phase red cell adhesion titers
Background and Objectives: Measurement of actual concentration of IgG requires methods like heat inactivation (HI) of serum. This study was aimed at comparing of heat-treated ABO titers performed by conventional test tube technique (CTT) and column agglutination technique (CAT) with hemagglutination...
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| Format: | Article |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Global Journal of Transfusion Medicine |
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| Online Access: | http://www.gjtmonline.com/article.asp?issn=2468-8398;year=2022;volume=7;issue=1;spage=54;epage=59;aulast=Ranjan |
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| author | Shweta Ranjan Prashant Pandey Divya Setya Supriya Kumari |
| author_facet | Shweta Ranjan Prashant Pandey Divya Setya Supriya Kumari |
| author_sort | Shweta Ranjan |
| collection | DOAJ |
| description | Background and Objectives: Measurement of actual concentration of IgG requires methods like heat inactivation (HI) of serum. This study was aimed at comparing of heat-treated ABO titers performed by conventional test tube technique (CTT) and column agglutination technique (CAT) with hemagglutination (HA)/solid-phase red cell adherence (SPRCA). Methods: This was a prospective, observational study conducted between October 2019 and March 2020. All consecutive A, B, and O group donors who gave consent for participation in the project were included. All samples were tested by CTT and CAT before and after HI (post DTT treatment performed by CTT [pCTT] and post DTT treatment performed by CAT [pCAT]) and with HA/SPRCA. Results: A total of 300 donors were included: 100 each with A, B, and O blood groups. For group O, IgG titers were higher than IgM titers among group O, whereas that among group A and group B, IgM titer was found to be higher than IgG titer by both HA/SPRCA and pCTT. The titer values among all the three blood groups were highest when the test was performed by pCAT, though the results of pCTT and SPRCA, pCTT, and pCAT were significant in group O donors. For groups A and B, results of pCTT and pCAT, pCAT, and HA/SPRCA were found to be statistically significant. Conclusion: Results obtained by HA/SPRCA were closer to pCTT, with respect to time consumption, expertise required, and lack of inter-observer variation. Titers obtained by pCAT were higher in comparison to pCTT obviously due to its relative higher sensitivity. Hence, these methods cannot be used interchangeably. |
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| id | doaj.art-8595b4bfc3e049f8b8c24e8516638e10 |
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| issn | 2468-8398 2455-8893 |
| language | English |
| last_indexed | 2024-04-12T13:16:38Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Global Journal of Transfusion Medicine |
| spelling | doaj.art-8595b4bfc3e049f8b8c24e8516638e102022-12-22T03:31:40ZengWolters Kluwer Medknow PublicationsGlobal Journal of Transfusion Medicine2468-83982455-88932022-01-0171545910.4103/gjtm.gjtm_65_21Comparative Evaluation of heat-inactivated ABO isoagglutinin titers with solid-phase red cell adhesion titersShweta RanjanPrashant PandeyDivya SetyaSupriya KumariBackground and Objectives: Measurement of actual concentration of IgG requires methods like heat inactivation (HI) of serum. This study was aimed at comparing of heat-treated ABO titers performed by conventional test tube technique (CTT) and column agglutination technique (CAT) with hemagglutination (HA)/solid-phase red cell adherence (SPRCA). Methods: This was a prospective, observational study conducted between October 2019 and March 2020. All consecutive A, B, and O group donors who gave consent for participation in the project were included. All samples were tested by CTT and CAT before and after HI (post DTT treatment performed by CTT [pCTT] and post DTT treatment performed by CAT [pCAT]) and with HA/SPRCA. Results: A total of 300 donors were included: 100 each with A, B, and O blood groups. For group O, IgG titers were higher than IgM titers among group O, whereas that among group A and group B, IgM titer was found to be higher than IgG titer by both HA/SPRCA and pCTT. The titer values among all the three blood groups were highest when the test was performed by pCAT, though the results of pCTT and SPRCA, pCTT, and pCAT were significant in group O donors. For groups A and B, results of pCTT and pCAT, pCAT, and HA/SPRCA were found to be statistically significant. Conclusion: Results obtained by HA/SPRCA were closer to pCTT, with respect to time consumption, expertise required, and lack of inter-observer variation. Titers obtained by pCAT were higher in comparison to pCTT obviously due to its relative higher sensitivity. Hence, these methods cannot be used interchangeably.http://www.gjtmonline.com/article.asp?issn=2468-8398;year=2022;volume=7;issue=1;spage=54;epage=59;aulast=Ranjancolumn agglutination technologycomparison of conventional tube techniquehemagglutination/solid-phase red cell adherenceheat inactivationisoagglutinin titer values |
| spellingShingle | Shweta Ranjan Prashant Pandey Divya Setya Supriya Kumari Comparative Evaluation of heat-inactivated ABO isoagglutinin titers with solid-phase red cell adhesion titers Global Journal of Transfusion Medicine column agglutination technology comparison of conventional tube technique hemagglutination/solid-phase red cell adherence heat inactivation isoagglutinin titer values |
| title | Comparative Evaluation of heat-inactivated ABO isoagglutinin titers with solid-phase red cell adhesion titers |
| title_full | Comparative Evaluation of heat-inactivated ABO isoagglutinin titers with solid-phase red cell adhesion titers |
| title_fullStr | Comparative Evaluation of heat-inactivated ABO isoagglutinin titers with solid-phase red cell adhesion titers |
| title_full_unstemmed | Comparative Evaluation of heat-inactivated ABO isoagglutinin titers with solid-phase red cell adhesion titers |
| title_short | Comparative Evaluation of heat-inactivated ABO isoagglutinin titers with solid-phase red cell adhesion titers |
| title_sort | comparative evaluation of heat inactivated abo isoagglutinin titers with solid phase red cell adhesion titers |
| topic | column agglutination technology comparison of conventional tube technique hemagglutination/solid-phase red cell adherence heat inactivation isoagglutinin titer values |
| url | http://www.gjtmonline.com/article.asp?issn=2468-8398;year=2022;volume=7;issue=1;spage=54;epage=59;aulast=Ranjan |
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