Codelivery of DOX and siRNA by folate-biotin-quaternized starch nanoparticles for promoting synergistic suppression of human lung cancer cells
In this paper, the self-assembled folate-biotin-quaternized starch nanoparticles (FBqS NPs) were used as carrier system of doxorubicin (DOX) and siRNAIGF1R for the codelivery of both into human lung adenocarcinoma cell lines (A549 cells) in vitro. The cytotoxicity, targeted ligand competition, cell...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Drug Delivery |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/10717544.2019.1606363 |
| _version_ | 1828869228278579200 |
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| author | Liangping Li Suoju He Lizhen Yu Ezzat H Elshazly Hui Wang Kuanmin Chen Song Zhang Lixia Ke Renmin Gong |
| author_facet | Liangping Li Suoju He Lizhen Yu Ezzat H Elshazly Hui Wang Kuanmin Chen Song Zhang Lixia Ke Renmin Gong |
| author_sort | Liangping Li |
| collection | DOAJ |
| description | In this paper, the self-assembled folate-biotin-quaternized starch nanoparticles (FBqS NPs) were used as carrier system of doxorubicin (DOX) and siRNAIGF1R for the codelivery of both into human lung adenocarcinoma cell lines (A549 cells) in vitro. The cytotoxicity, targeted ligand competition, cell proliferation inhibition, cellular uptake, endocytosis mechanism and target protein suppression of drug-loaded FBqS NPs were evaluated in detail. Compared with several other drug formulations under same condition, siRNAIGF1R/DOX/FBqS NPs exhibited the greatest cytotoxicity to A549 cells and the cytotoxicity was competitively inhibited by free folate in dose-dependent manner. The A549 cells treated by siRNAIGF1R/DOX/FBqS NPs showed the lowest cell proliferation capacity. The energy-dependent clathrin- and caveolae-mediated endocytosis might be the primary cellular uptake mechanism of drug-loaded FBqS NPs. The expression of IGF1R protein in A549 cells treated by siRNAIGF1R/FBqS NPs declined dramatically. So the FBqS NPs were expected as the co-carrier system of chemotherapeutants and siRNAs for future clinical application. |
| first_indexed | 2024-12-13T05:46:36Z |
| format | Article |
| id | doaj.art-8597730635c943b0bba1ba50402932c3 |
| institution | Directory Open Access Journal |
| issn | 1071-7544 1521-0464 |
| language | English |
| last_indexed | 2024-12-13T05:46:36Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Drug Delivery |
| spelling | doaj.art-8597730635c943b0bba1ba50402932c32022-12-21T23:57:39ZengTaylor & Francis GroupDrug Delivery1071-75441521-04642019-01-0126149950810.1080/10717544.2019.16063631606363Codelivery of DOX and siRNA by folate-biotin-quaternized starch nanoparticles for promoting synergistic suppression of human lung cancer cellsLiangping Li0Suoju He1Lizhen Yu2Ezzat H Elshazly3Hui Wang4Kuanmin Chen5Song Zhang6Lixia Ke7Renmin Gong8College of Life Science, Anhui Normal UniversityCollege of Life Science, Anhui Normal UniversityCollege of Life Science, Anhui Normal UniversityCollege of Life Science, Anhui Normal UniversityCollege of Life Science, Anhui Normal UniversityCollege of Life Science, Anhui Normal UniversityCollege of Life Science, Anhui Normal UniversityCollege of Life Science, Anhui Normal UniversityCollege of Life Science, Anhui Normal UniversityIn this paper, the self-assembled folate-biotin-quaternized starch nanoparticles (FBqS NPs) were used as carrier system of doxorubicin (DOX) and siRNAIGF1R for the codelivery of both into human lung adenocarcinoma cell lines (A549 cells) in vitro. The cytotoxicity, targeted ligand competition, cell proliferation inhibition, cellular uptake, endocytosis mechanism and target protein suppression of drug-loaded FBqS NPs were evaluated in detail. Compared with several other drug formulations under same condition, siRNAIGF1R/DOX/FBqS NPs exhibited the greatest cytotoxicity to A549 cells and the cytotoxicity was competitively inhibited by free folate in dose-dependent manner. The A549 cells treated by siRNAIGF1R/DOX/FBqS NPs showed the lowest cell proliferation capacity. The energy-dependent clathrin- and caveolae-mediated endocytosis might be the primary cellular uptake mechanism of drug-loaded FBqS NPs. The expression of IGF1R protein in A549 cells treated by siRNAIGF1R/FBqS NPs declined dramatically. So the FBqS NPs were expected as the co-carrier system of chemotherapeutants and siRNAs for future clinical application.http://dx.doi.org/10.1080/10717544.2019.1606363folate-biotin-quaternized starch nanoparticledoxorubicinsirnacodeliveryhuman lung cancer cell lines |
| spellingShingle | Liangping Li Suoju He Lizhen Yu Ezzat H Elshazly Hui Wang Kuanmin Chen Song Zhang Lixia Ke Renmin Gong Codelivery of DOX and siRNA by folate-biotin-quaternized starch nanoparticles for promoting synergistic suppression of human lung cancer cells Drug Delivery folate-biotin-quaternized starch nanoparticle doxorubicin sirna codelivery human lung cancer cell lines |
| title | Codelivery of DOX and siRNA by folate-biotin-quaternized starch nanoparticles for promoting synergistic suppression of human lung cancer cells |
| title_full | Codelivery of DOX and siRNA by folate-biotin-quaternized starch nanoparticles for promoting synergistic suppression of human lung cancer cells |
| title_fullStr | Codelivery of DOX and siRNA by folate-biotin-quaternized starch nanoparticles for promoting synergistic suppression of human lung cancer cells |
| title_full_unstemmed | Codelivery of DOX and siRNA by folate-biotin-quaternized starch nanoparticles for promoting synergistic suppression of human lung cancer cells |
| title_short | Codelivery of DOX and siRNA by folate-biotin-quaternized starch nanoparticles for promoting synergistic suppression of human lung cancer cells |
| title_sort | codelivery of dox and sirna by folate biotin quaternized starch nanoparticles for promoting synergistic suppression of human lung cancer cells |
| topic | folate-biotin-quaternized starch nanoparticle doxorubicin sirna codelivery human lung cancer cell lines |
| url | http://dx.doi.org/10.1080/10717544.2019.1606363 |
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