Antimicrobial Resistance Profiles of <i>Escherichia coli</i> from Diarrheic Weaned Piglets after the Ban on Antibiotic Growth Promoters in Feed
This study aimed to survey the antimicrobial resistance profiles of 690 pathogenic <i>Escherichia coli</i> isolates obtained from Korean pigs with symptoms of enteric colibacillosis between 2007 and 2017, while assessing the change in antimicrobial resistance profiles before and after th...
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| Format: | Article |
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MDPI AG
2020-10-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/9/11/755 |
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| author | Do Kyung-Hyo Byun Jae-Won Lee Wan-Kyu |
| author_facet | Do Kyung-Hyo Byun Jae-Won Lee Wan-Kyu |
| author_sort | Do Kyung-Hyo |
| collection | DOAJ |
| description | This study aimed to survey the antimicrobial resistance profiles of 690 pathogenic <i>Escherichia coli</i> isolates obtained from Korean pigs with symptoms of enteric colibacillosis between 2007 and 2017, while assessing the change in antimicrobial resistance profiles before and after the ban on antibiotic growth promoters (AGPs). Following the Clinical and Laboratory Standards Institute guidelines, the antimicrobial resistance phenotype was analyzed through the disk diffusion method, and the genotype was analyzed by the polymerase chain reaction. After the ban on AGPs, resistance to gentamicin (from 68.8% to 39.0%), neomycin (from 84.9% to 57.8%), ciprofloxacin (from 49.5% to 39.6%), norfloxacin (from 46.8% to 37.3%), and amoxicillin/clavulanic acid (from 40.8% to 23.5%) decreased compared to before the ban. However, resistance to cephalothin (from 51.4% to 66.5%), cefepime (from 0.0% to 2.4%), and colistin (from 7.3% to 11.0%) had increased. We confirmed a high percentage of multidrug resistance before (95.0%) and after (96.6%) the ban on AGPs. The <i>AmpC</i> gene was the most prevalent from 2007 to 2017 (60.0%), followed by the <i>blaTEM</i> gene (55.5%). The <i>blaTEM</i> was prevalent before (2007–2011, 69.3%) and after (2012–2017, 49.2%) the ban on AGPs. These results provide data that can be used for the prevention and treatment of enteric colibacillosis. |
| first_indexed | 2024-03-10T15:14:02Z |
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| id | doaj.art-859a25b5571d45788f818bc981c9e418 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T15:14:02Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-859a25b5571d45788f818bc981c9e4182023-11-20T19:06:48ZengMDPI AGAntibiotics2079-63822020-10-0191175510.3390/antibiotics9110755Antimicrobial Resistance Profiles of <i>Escherichia coli</i> from Diarrheic Weaned Piglets after the Ban on Antibiotic Growth Promoters in FeedDo Kyung-Hyo0Byun Jae-Won1Lee Wan-Kyu2Department of Veterinary Bacteriology and Infectious Diseases, College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, KoreaAnimal Disease Diagnostic Division, Animal and Plant Quarantine Agency, Gimcheon 39660, KoreaDepartment of Veterinary Bacteriology and Infectious Diseases, College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, KoreaThis study aimed to survey the antimicrobial resistance profiles of 690 pathogenic <i>Escherichia coli</i> isolates obtained from Korean pigs with symptoms of enteric colibacillosis between 2007 and 2017, while assessing the change in antimicrobial resistance profiles before and after the ban on antibiotic growth promoters (AGPs). Following the Clinical and Laboratory Standards Institute guidelines, the antimicrobial resistance phenotype was analyzed through the disk diffusion method, and the genotype was analyzed by the polymerase chain reaction. After the ban on AGPs, resistance to gentamicin (from 68.8% to 39.0%), neomycin (from 84.9% to 57.8%), ciprofloxacin (from 49.5% to 39.6%), norfloxacin (from 46.8% to 37.3%), and amoxicillin/clavulanic acid (from 40.8% to 23.5%) decreased compared to before the ban. However, resistance to cephalothin (from 51.4% to 66.5%), cefepime (from 0.0% to 2.4%), and colistin (from 7.3% to 11.0%) had increased. We confirmed a high percentage of multidrug resistance before (95.0%) and after (96.6%) the ban on AGPs. The <i>AmpC</i> gene was the most prevalent from 2007 to 2017 (60.0%), followed by the <i>blaTEM</i> gene (55.5%). The <i>blaTEM</i> was prevalent before (2007–2011, 69.3%) and after (2012–2017, 49.2%) the ban on AGPs. These results provide data that can be used for the prevention and treatment of enteric colibacillosis.https://www.mdpi.com/2079-6382/9/11/755<i>Escherichia coli</i>antimicrobial resistanceswineweaned pigletantibiotic growth promoters |
| spellingShingle | Do Kyung-Hyo Byun Jae-Won Lee Wan-Kyu Antimicrobial Resistance Profiles of <i>Escherichia coli</i> from Diarrheic Weaned Piglets after the Ban on Antibiotic Growth Promoters in Feed Antibiotics <i>Escherichia coli</i> antimicrobial resistance swine weaned piglet antibiotic growth promoters |
| title | Antimicrobial Resistance Profiles of <i>Escherichia coli</i> from Diarrheic Weaned Piglets after the Ban on Antibiotic Growth Promoters in Feed |
| title_full | Antimicrobial Resistance Profiles of <i>Escherichia coli</i> from Diarrheic Weaned Piglets after the Ban on Antibiotic Growth Promoters in Feed |
| title_fullStr | Antimicrobial Resistance Profiles of <i>Escherichia coli</i> from Diarrheic Weaned Piglets after the Ban on Antibiotic Growth Promoters in Feed |
| title_full_unstemmed | Antimicrobial Resistance Profiles of <i>Escherichia coli</i> from Diarrheic Weaned Piglets after the Ban on Antibiotic Growth Promoters in Feed |
| title_short | Antimicrobial Resistance Profiles of <i>Escherichia coli</i> from Diarrheic Weaned Piglets after the Ban on Antibiotic Growth Promoters in Feed |
| title_sort | antimicrobial resistance profiles of i escherichia coli i from diarrheic weaned piglets after the ban on antibiotic growth promoters in feed |
| topic | <i>Escherichia coli</i> antimicrobial resistance swine weaned piglet antibiotic growth promoters |
| url | https://www.mdpi.com/2079-6382/9/11/755 |
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